PI3 Kinase Class III Antibody | Affinity Biosciences

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Product:	PI3 Kinase Class III Antibody
Catalog:	AF7921
Description:	Rabbit polyclonal antibody to PI3 Kinase Class III
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	100kDa; 102kD(Calculated).
Uniprot:	Q8NEB9
RRID:	AB_2844284

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(91%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

PI3 Kinase Class III Antibody detects endogenous levels of total PI3 Kinase Class III.

RRID:

AB_2844284
Cite Format: Affinity Biosciences Cat# AF7921, RRID:AB_2844284.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

hVps34; MGC61518; Phosphatidylinositol 3 kinase catalytic subunit type 3; Phosphatidylinositol 3 kinase class 3; Phosphatidylinositol 3 kinase p100 subunit; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide 3 kinase class 3; Phosphoinositide-3-kinase class 3; PI3 kinase type 3; PI3-kinase type 3; PI3K type 3; Pik3c3; PK3C3_HUMAN; PtdIns 3 kinase type 3; PtdIns-3-kinase type 3; Vps 34; Vps34;

Immunogens

Immunogen:

Uniprot:

Q8NEB9(PK3C3_HUMAN) >>Visit HPA database.

Gene(ID):

PIK3C3(5289)

Expression:

Q8NEB9 PK3C3_HUMAN:

Ubiquitously expressed, with a highest expression in skeletal muscle.

Sequence:

Q8NEB9 PK3C3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGEAEKFHYIYSCDLDINVQLKIGSLEGKREQKSYKAVLEDPMLKFSGLYQETCSDLYVTCQVFAEGKPLALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNAQVALTIWDVYGPGKAVPVGGTTVSLFGKYGMFRQGMHDLKVWPNVEADGSEPTKTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQMSMENLVESKHHKLARSLRSGPSDHDLKPNAATRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFLKCVNWDLPQEAKQALELLGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALKYENFDDIKNGLEPTKKDSQSSVSENVSNSGINSAEIDSSQIITSPLPSVSSPPPASKTKEVPDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQDTQQRDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKAVQRESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPETATLFKSALMPAQLFFKTEDGGKYPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQNFFRKYAPSENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYILGRDPKPLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Pig

Chicken

Xenopus

Zebrafish

Dog

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NEB9 As Substrate

Q8NEB9

Site	PTM Type	Enzyme	Source
S25	Phosphorylation		Uniprot
K29	Acetylation		Uniprot
K29	Ubiquitination		Uniprot
K36	Methylation		Uniprot
K36	Ubiquitination		Uniprot
K96	Ubiquitination		Uniprot
K132	Ubiquitination		Uniprot
K144	Ubiquitination		Uniprot
K158	Ubiquitination		Uniprot
T159	Phosphorylation	P06493 (CDK1) , Q00535 (CDK5)	Uniprot
T163	Phosphorylation		Uniprot
S164	Phosphorylation		Uniprot
S165	Phosphorylation		Uniprot
S168	Phosphorylation		Uniprot
K209	Ubiquitination		Uniprot
K229	Ubiquitination		Uniprot
Y231	Phosphorylation		Uniprot
K238	Ubiquitination		Uniprot
S244	Phosphorylation		Uniprot
S249	Phosphorylation		Uniprot
S261	Phosphorylation		Uniprot
S268	Phosphorylation		Uniprot
K269	Ubiquitination		Uniprot
S276	Phosphorylation		Uniprot
S282	Phosphorylation		Uniprot
K287	Methylation		Uniprot
K287	Ubiquitination		Uniprot
T305	Phosphorylation		Uniprot
Y310	Phosphorylation		Uniprot
Y321	Phosphorylation		Uniprot
Y322	Phosphorylation		Uniprot
T324	Phosphorylation		Uniprot
K328	Ubiquitination		Uniprot
K346	Ubiquitination		Uniprot
Y379	Phosphorylation		Uniprot
Y395	Phosphorylation		Uniprot
K412	Ubiquitination		Uniprot
S448	Phosphorylation		Uniprot
S454	Phosphorylation		Uniprot
S455	Phosphorylation		Uniprot
K463	Ubiquitination		Uniprot
T488	Phosphorylation		Uniprot
K582	Ubiquitination		Uniprot
K600	Ubiquitination		Uniprot
K613	Methylation		Uniprot
T625	Phosphorylation		Uniprot
K630	Ubiquitination		Uniprot
Y631	Phosphorylation		Uniprot
K666	Ubiquitination		Uniprot
T668	Phosphorylation	Q00535 (CDK5)	Uniprot
T677	Phosphorylation		Uniprot
K706	Ubiquitination		Uniprot
K771	Acetylation		Uniprot
K781	Acetylation		Uniprot
K781	Ubiquitination		Uniprot
Y803	Phosphorylation		Uniprot
K840	Sumoylation		Uniprot
K845	Sumoylation		Uniprot
Y884	Phosphorylation		Uniprot

Research Backgrounds

Q8NEB9_PK3C3_HUMAN

Function:

Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity).

Subcellular Location:

Midbody. Late endosome. Cytoplasmic vesicle>Autophagosome.
Note: As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (Probable). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity).

Tissue Specificity:

Ubiquitously expressed, with a highest expression in skeletal muscle.

Subunit Structure:

Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxilliary subunits to form alternative complex forms. Alternative complex forms containing a forth regulatory subunit in a mutually exclusive manner are: the PI3K complex I (PI3KC3-C1) containing ATG14, and the PI3K complex II (PI3KC3-C2) containing UVRAG. PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex. Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits such as RUBCN, SH3GLB1/Bif-1 and AMBRA1. PI3KC3-C1 probably associates with PIK3CB (By similarity). Interacts with RAB7A in the presence of PIK3R4. Interacts with AMBRA1 (By similarity). Interacts with BECN1P1/BECN2. Interacts with SLAMF1. May be a component of a complex composed of RAB5A (in GDP-bound form), DYN2 and PIK3C3 (By similarity). Interacts with NCKAP1L.

Family&Domains:

Belongs to the PI3/PI4-kinase family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). DADLE promotes motor function recovery by inhibiting cytosolic phospholipase A2 mediated lysosomal membrane permeabilization after spinal cord injury. British journal of pharmacology, 2024 (PubMed: 37766498) [IF=7.3]

2). The potential immunotoxicity of fine particulate matter based on SD rat spleen. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2019 (PubMed: 31218585) [IF=5.8]

Application: WB Species: rat Sample: spleen

Fig. 6 |PM2.5 exposure led to the autophagy of spleen. a, b LC3,p62, and VPS34 expressions were determined by Western blot assay and statistical analysis of LC3,p62, and VPS34 expressions by summer PM2.5 treatment in SD Rats.

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PI3 Kinase Class III Antibody - #AF7921