NCoA2 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of NCoA2 in lysates of HeLa , using NCoA2 Antibody(AF7894).

Product:	NCoA2 Antibody
Catalog:	AF7894
Description:	Rabbit polyclonal antibody to NCoA2
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	175kDa; 159kD(Calculated).
Uniprot:	Q15596
RRID:	AB_2844258

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

NCoA2 Antibody detects endogenous levels of total NCoA2.

RRID:

AB_2844258
Cite Format: Affinity Biosciences Cat# AF7894, RRID:AB_2844258.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

bHLHe75; Class E basic helix-loop-helix protein 75; glucocorticoid receptor interacting protein 1; GRIP 1; GRIP1; hTIF2; MED1; MGC138808; NCoA 2; NCoA-2; Ncoa2; NCOA2_HUMAN; Nuclear receptor coactivator 2; Oncogene FGR; p160; SRC 2; src2; Steroid receptor coactivator 2; TIF 2; TIF2; Transcriptional intermediary factor 2;

Immunogens

Immunogen:

Uniprot:

Q15596(NCOA2_HUMAN) >>Visit HPA database.

Gene(ID):

NCOA2(10499)

Sequence:

Q15596 NCOA2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGMGENTSDPSRAETRKRKECPDQLGPSPKRNTEKRNREQENKYIEELAELIFANFNDIDNFNFKPDKCAILKETVKQIRQIKEQEKAAAANIDEVQKSDVSSTGQGVIDKDALGPMMLEALDGFFFVVNLEGNVVFVSENVTQYLRYNQEELMNKSVYSILHVGDHTEFVKNLLPKSIVNGGSWSGEPPRRNSHTFNCRMLVKPLPDSEEEGHDNQEAHQKYETMQCFAVSQPKSIKEEGEDLQSCLICVARRVPMKERPVLPSSESFTTRQDLQGKITSLDTSTMRAAMKPGWEDLVRRCIQKFHAQHEGESVSYAKRHHHEVLRQGLAFSQIYRFSLSDGTLVAAQTKSKLIRSQTTNEPQLVISLHMLHREQNVCVMNPDLTGQTMGKPLNPISSNSPAHQALCSGNPGQDMTLSSNINFPINGPKEQMGMPMGRFGGSGGMNHVSGMQATTPQGSNYALKMNSPSQSSPGMNPGQPTSMLSPRHRMSPGVAGSPRIPPSQFSPAGSLHSPVGVCSSTGNSHSYTNSSLNALQALSEGHGVSLGSSLASPDLKMGNLQNSPVNMNPPPLSKMGSLDSKDCFGLYGEPSEGTTGQAESSCHPGEQKETNDPNLPPAVSSERADGQSRLHDSKGQTKLLQLLTTKSDQMEPSPLASSLSDTNKDSTGSLPGSGSTHGTSLKEKHKILHRLLQDSSSPVDLAKLTAEATGKDLSQESSSTAPGSEVTIKQEPVSPKKKENALLRYLLDKDDTKDIGLPEITPKLERLDSKTDPASNTKLIAMKTEKEEMSFEPGDQPGSELDNLEEILDDLQNSQLPQLFPDTRPGAPAGSVDKQAIINDLMQLTAENSPVTPVGAQKTALRISQSTFNNPRPGQLGRLLPNQNLPLDITLQSPTGAGPFPPIRNSSPYSVIPQPGMMGNQGMIGNQGNLGNSSTGMIGNSASRPTMPSGEWAPQSSAVRVTCAATTSAMNRPVQGGMIRNPAASIPMRPSSQPGQRQTLQSQVMNIGPSELEMNMGGPQYSQQQAPPNQTAPWPESILPIDQASFASQNRQPFGSSPDDLLCPHPAAESPSDEGALLDQLYLALRNFDGLEEIDRALGIPELVSQSQAVDPEQFSSQDSNIMLEQKAPVFPQQYASQAQMAQGSYSPMQDPNFHTMGQRPSYATLRMQPRPGLRPTGLVQNQPNQLRLQLQHRLQAQQNRQPLMNQISNVSNVNLTLRPGVPTQAPINAQMLAQRQREILNQHLRQRQMHQQQQVQQRTLMMRGQGLNMTPSMVAPSGMPATMSNPRIPQANAQQFPFPPNYGISQQPDPGFTGATTPQSPLMSPRMAHTQSPMMQQSQANPAYQAPSDINGWAQGNMGGNSMFSQQSPPHFGQQANTSMYSNNMNINVSMATNTGGMSSMNQMTGQISMTSVTSVPTSGLSSMGPEQVNDPALRGGNLFPNQLPGMDMIKQEGDTTRKYC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Rabbit

100

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15596 As Substrate

Q15596

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
S2	Phosphorylation		Uniprot
T8	Phosphorylation		Uniprot
S29	Phosphorylation		Uniprot
K31	Acetylation		Uniprot
T76	Phosphorylation		Uniprot
K78	Acetylation		Uniprot
K99	Ubiquitination		Uniprot
S161	Phosphorylation		Uniprot
S195	Phosphorylation		Uniprot
K205	Ubiquitination		Uniprot
S210	Phosphorylation		Uniprot
K239	Sumoylation		Uniprot
K279	Ubiquitination		Uniprot
K293	Ubiquitination		Uniprot
K306	Ubiquitination		Uniprot
K320	Ubiquitination		Uniprot
T456	Phosphorylation		Uniprot
T457	Phosphorylation		Uniprot
S469	Phosphorylation	P68400 (CSNK2A1) , P50750 (CDK9)	Uniprot
S471	Phosphorylation		Uniprot
S474	Phosphorylation		Uniprot
S487	Phosphorylation	P50750 (CDK9) , P68400 (CSNK2A1)	Uniprot
R489	Methylation		Uniprot
S493	Phosphorylation	P50750 (CDK9) , P68400 (CSNK2A1)	Uniprot
S499	Phosphorylation	P50750 (CDK9) , P68400 (CSNK2A1)	Uniprot
R501	Methylation		Uniprot
S554	Phosphorylation		Uniprot
S565	Phosphorylation		Uniprot
S579	Phosphorylation		Uniprot
K636	Acetylation		Uniprot
K640	Acetylation		Uniprot
K640	Sumoylation		Uniprot
K640	Ubiquitination		Uniprot
S655	Phosphorylation		Uniprot
S660	Phosphorylation		Uniprot
S668	Phosphorylation		Uniprot
T669	Phosphorylation		Uniprot
S671	Phosphorylation		Uniprot
S675	Phosphorylation		Uniprot
T681	Phosphorylation		Uniprot
S682	Phosphorylation		Uniprot
S697	Phosphorylation		Uniprot
S698	Phosphorylation		Uniprot
S699	Phosphorylation		Uniprot
K705	Methylation		Uniprot
K705	Ubiquitination		Uniprot
K713	Acetylation		Uniprot
S716	Phosphorylation		Uniprot
S719	Phosphorylation		Uniprot
S721	Phosphorylation		Uniprot
T722	Phosphorylation		Uniprot
S726	Phosphorylation		Uniprot
T729	Phosphorylation		Uniprot
K731	Sumoylation		Uniprot
S736	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1) , Q16539 (MAPK14)	Uniprot
K738	Acetylation		Uniprot
K739	Acetylation		Uniprot
Y747	Phosphorylation		Uniprot
K765	Sumoylation		Uniprot
S771	Phosphorylation		Uniprot
T773	Phosphorylation		Uniprot
K780	Acetylation		Uniprot
K785	Acetylation		Uniprot
K785	Sumoylation		Uniprot
K785	Ubiquitination		Uniprot
K788	Sumoylation		Uniprot
T847	Phosphorylation		Uniprot
S851	Phosphorylation		Uniprot
T854	Phosphorylation		Uniprot
R874	Methylation		Uniprot
K1129	Sumoylation		Uniprot
R1162	Methylation		Uniprot
R1169	Methylation		Uniprot
R1173	Methylation		Uniprot
R1177	Methylation		Uniprot
R1190	Methylation		Uniprot
R1196	Methylation		Uniprot
R1203	Methylation		Uniprot
R1221	Methylation		Uniprot
R1240	Methylation		Uniprot
R1248	Methylation		Uniprot
R1250	Methylation		Uniprot
R1261	Methylation		Uniprot
R1266	Methylation		Uniprot
K1454	Sumoylation		Uniprot

Research Backgrounds

Q15596_NCOA2_HUMAN

Function:

Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).

Subcellular Location:

Nucleus.

Subunit Structure:

Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts (via C-terminus) with CREBBP. Interacts with ESR1, HIF1A, NCOA1, APEX, NR3C1, NR3C2, CARM1, RARA, and RXRA. Present in a complex containing CARM1 and EP300/P300. Interacts with CASP8AP2 and TTLL5/STAMP. Interacts with PSMB9 and DDX5. Interacts (via LXXLL 1, 2 and 3 motifs) with RORA and RORC (via AF-2 motif). Interacts with RWDD3. Interacts with CLOCK and ARNTL/BMAL1 (By similarity). Interacts with NR4A3; potentiates the activity of the NR4A3 (By similarity). Interacts with NR1H3.

Family&Domains:

Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.

The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.

Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.

Belongs to the SRC/p160 nuclear receptor coactivator family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

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NCoA2 Antibody - #AF7894