MEF2D Antibody | Affinity Biosciences

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Western blot analysis of MEF2D in lysates of HepG2 , using MEF2D Antibody(AF7888).

Western blot analysis of extracts from RAW264.

Western blot analysis of extracts from Mouse testis, using MEF2D Antibody at 1/1000 dilution.

Product:	MEF2D Antibody
Catalog:	AF7888
Description:	Rabbit polyclonal antibody to MEF2D
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	56kDa; 56kD(Calculated).
Uniprot:	Q14814
RRID:	AB_2844252

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

MEF2D Antibody detects endogenous levels of total MEF2D.

RRID:

AB_2844252
Cite Format: Affinity Biosciences Cat# AF7888, RRID:AB_2844252.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp686I1536; MADS box transcription factor 2 polypeptide D; Mef2d; MEF2D_HUMAN; Myocyte enhancer factor 2D; Myocyte specific enhancer factor 2, polypeptide D; Myocyte specific enhancer factor 2D; Myocyte-specific enhancer factor 2D;

Immunogens

Immunogen:

Uniprot:

Q14814(MEF2D_HUMAN) >>Visit HPA database.

Gene(ID):

MEF2D(4209)

Sequence:

Q14814 MEF2D_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGRKKIQIQRITDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNHSNKLFQYASTDMDKVLLKYTEYNEPHESRTNADIIETLRKKGFNGCDSPEPDGEDSLEQSPLLEDKYRRASEELDGLFRRYGSTVPAPNFAMPVTVPVSNQSSLQFSNPSGSLVTPSLVTSSLTDPRLLSPQQPALQRNSVSPGLPQRPASAGAMLGGDLNSANGACPSPVGNGYVSARASPGLLPVANGNSLNKVIPAKSPPPPTHSTQLGAPSRKPDLRVITSQAGKGLMHHLTEDHLDLNNAQRLGVSQSTHSLTTPVVSVATPSLLSQGLPFSSMPTAYNTDYQLTSAELSSLPAFSSPGGLSLGNVTAWQQPQQPQQPQQPQPPQQQPPQPQQPQPQQPQQPQQPPQQQSHLVPVSLSNLIPGSPLPHVGAALTVTTHPHISIKSEPVSPSRERSPAPPPPAVFPAARPEPGDGLSSPAGGSYETGDRDDGRGDFGPTLGLLRPAPEPEAEGSAVKRMRLDTWTLK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Zebrafish

100

Rabbit

100

Chicken

Xenopus

Bovine

Sheep

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14814 As Substrate

Q14814

Site	PTM Type	Enzyme	Source
K23	Ubiquitination		Uniprot
K64	Ubiquitination		Uniprot
K68	Ubiquitination		Uniprot
S98	Phosphorylation	P11802 (CDK4)	Uniprot
S106	Phosphorylation		Uniprot
S110	Phosphorylation	P11802 (CDK4)	Uniprot
Y117	Phosphorylation		Uniprot
S121	Phosphorylation	P17612 (PRKACA)	Uniprot
S180	Phosphorylation	Q13164 (MAPK7)	Uniprot
S190	Phosphorylation	P17612 (PRKACA)	Uniprot
S192	Phosphorylation		Uniprot
S201	Phosphorylation		Uniprot
S212	Phosphorylation		Uniprot
S219	Phosphorylation		Uniprot
S227	Phosphorylation		Uniprot
S231	Phosphorylation		Uniprot
S242	Phosphorylation		Uniprot
K245	Acetylation		Uniprot
K250	Ubiquitination		Uniprot
S251	Phosphorylation		Uniprot
T256	Phosphorylation		Uniprot
K267	Methylation		Uniprot
S275	O-Glycosylation		Uniprot
T286	Phosphorylation		Uniprot
K439	Acetylation		Uniprot
K439	Sumoylation		Uniprot
S444	Phosphorylation	Q00535 (CDK5)	Uniprot
S446	Phosphorylation		Uniprot
S450	Phosphorylation		Uniprot
S471	Phosphorylation		Uniprot
S472	Phosphorylation		Uniprot
S508	Phosphorylation		Uniprot
K511	Acetylation		Uniprot
T517	Phosphorylation		Uniprot
K521	Acetylation		Uniprot

Research Backgrounds

Q14814_MEF2D_HUMAN

Function:

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis (By similarity).

PTMs:

Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phosphorylation on Ser-180 can be enhanced by EGF. Phosphorylated and activated by CaMK4.

Acetylated on Lys-439 by CREBBP. Acetylated by EP300. Deacetylated by SIRT1 and HDAC3.

Sumoylated on Lys-439 with SUMO2 but not SUMO1; which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3.

Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation (By similarity).

Subcellular Location:

Nucleus.
Note: Translocated by HDAC4 to nuclear dots.

Subunit Structure:

Interacts with MYOG (By similarity). Forms a complex with class II HDACs in undifferentiating cells. On myogenic differentiation, HDACs are released into the cytoplasm allowing MEF2s to interact with other proteins for activation. Interacts with HDAC4 (in undifferentiating cells); the interaction translocates MEF2D to nuclear dots. Forms a heterodimer with MEF2A. Interacts with MAPK7; the interaction phosphorylates but does not activate MEF2D (By similarity). Interacts with CCAR2 and HDAC3.

Family&Domains:

The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.

Belongs to the MEF2 family.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

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MEF2D Antibody - #AF7888