FOXM1 Antibody | Affinity Biosciences

IF/ICC
Cites

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Figure 5 Dysfunction of gene foxm1 impairs the proliferation of HSPCs (A) Mitotic malformation of HSPCs in foxm1 morphants at 33 hpf (AGM) and 72 hpf (CHT), respectively.

Product:	FOXM1 Antibody
Catalog:	AF7860
Description:	Rabbit polyclonal antibody to FOXM1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	84kD, 100-130kD; 84kD(Calculated).
Uniprot:	Q08050
RRID:	AB_2844224

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(90%), Zebrafish(100%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

FOXM1 Antibody detects endogenous levels of total FOXM1.

RRID:

AB_2844224
Cite Format: Affinity Biosciences Cat# AF7860, RRID:AB_2844224.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FKHL16; Forkhead box M1; Forkhead box protein M1; forkhead like 16; Forkhead-related protein FKHL16; FOX M1; Foxm1; FOXM1_HUMAN; FOXM1B; Hepatocyte nuclear factor 3 forkhead homolog 11; HFH-11; HFH11; HNF-3/fork-head homolog 11; HNF3; INS1; M phase phosphoprotein 2; M-phase phosphoprotein 2; MPHOSPH2; MPM-2 reactive phosphoprotein 2; MPP2; PIG29; TGT3; Transcription factor Trident; Trident; WIN; Winged-helix factor from INS-1 cells; Winged-helix factor from INS1 cells;

Immunogens

Immunogen:

Uniprot:

Q08050(FOXM1_HUMAN) >>Visit HPA database.

Gene(ID):

FOXM1(2305)

Expression:

Q08050 FOXM1_HUMAN:

Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis.

Sequence:

Q08050 FOXM1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKTSPRRPLILKRRRLPLPVQNAPSETSEEEPKRSPAQQESNQAEASKEVAESNSCKFPAGIKIINHPTMPNTQVVAIPNNANIHSIITALTAKGKESGSSGPNKFILISCGGAPTQPPGLRPQTQTSYDAKRTEVTLETLGPKPAARDVNLPRPPGALCEQKRETCADGEAAGCTINNSLSNIQWLRKMSSDGLGSRSIKQEMEEKENCHLEQRQVKVEEPSRPSASWQNSVSERPPYSYMAMIQFAINSTERKRMTLKDIYTWIEDHFPYFKHIAKPGWKNSIRHNLSLHDMFVRETSANGKVSFWTIHPSANRYLTLDQVFKPLDPGSPQLPEHLESQQKRPNPELRRNMTIKTELPLGARRKMKPLLPRVSSYLVPIQFPVNQSLVLQPSVKVPLPLAASLMSSELARHSKRVRIAPKVLLAEEGIAPLSSAGPGKEEKLLFGEGFSPLLPVQTIKEEEIQPGEEMPHLARPIKVESPPLEEWPSPAPSFKEESSHSWEDSSQSPTPRPKKSYSGLRSPTRCVSEMLVIQHRERRERSRSRRKQHLLPPCVDEPELLFSEGPSTSRWAAELPFPADSSDPASQLSYSQEVGGPFKTPIKETLPISSTPSKSVLPRTPESWRLTPPAKVGGLDFSPVQTSQGASDPLPDPLGLMDLSTTPLQSAPPLESPQRLLSSEPLDLISVPFGNSSPSDIDVPKPGSPEPQVSGLAANRSLTEGLVLDTMNDSLSKILLDISFPGLDEDPLGPDNINWSQFIPELQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Rabbit

100

Pig

Horse

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q08050 As Substrate

Q08050

Site	PTM Type	Enzyme	Source
S4	Phosphorylation	Q00534 (CDK6) , P11802 (CDK4)	Uniprot
S35	Phosphorylation	P11802 (CDK4) , Q00534 (CDK6)	Uniprot
S41	Phosphorylation		Uniprot
K57	Ubiquitination		Uniprot
K63	Acetylation		Uniprot
K189	Ubiquitination		Uniprot
S191	Phosphorylation		Uniprot
K201	Sumoylation		Uniprot
K218	Sumoylation		Uniprot
S251	Phosphorylation	P06493 (CDK1)	Uniprot
K278	Methylation		Uniprot
K278	Ubiquitination		Uniprot
K282	Methylation		Uniprot
S331	Phosphorylation		Uniprot
T354	Phosphorylation		Uniprot
K356	Sumoylation		Uniprot
T357	Phosphorylation		Uniprot
S376	Phosphorylation	O96017 (CHEK2)	Uniprot
K422	Acetylation		Uniprot
K422	Sumoylation		Uniprot
K422	Ubiquitination		Uniprot
K440	Acetylation		Uniprot
K440	Sumoylation		Uniprot
K440	Ubiquitination		Uniprot
S451	Phosphorylation	Q00534 (CDK6) , P11802 (CDK4)	Uniprot
K460	Sumoylation		Uniprot
K478	Sumoylation		Uniprot
S481	Phosphorylation		Uniprot
S489	Phosphorylation	Q00534 (CDK6) , P11802 (CDK4)	Uniprot
K495	Sumoylation		Uniprot
S501	Phosphorylation		Uniprot
S508	Phosphorylation	Q00534 (CDK6) , P11802 (CDK4)	Uniprot
T510	Phosphorylation	P11802 (CDK4) , Q00534 (CDK6)	Uniprot
Y517	Phosphorylation		Uniprot
S522	Phosphorylation	Q00534 (CDK6) , P11802 (CDK4)	Uniprot
T524	Phosphorylation		Uniprot
T600	Phosphorylation	P11802 (CDK4) , P06493 (CDK1) , Q00534 (CDK6)	Uniprot
K603	Acetylation		Uniprot
K603	Sumoylation		Uniprot
T611	Phosphorylation	P11802 (CDK4) , P06493 (CDK1) , Q00534 (CDK6)	Uniprot
K614	Acetylation		Uniprot
T620	Phosphorylation	Q00534 (CDK6) , P11802 (CDK4) , P06493 (CDK1)	Uniprot
S623	Phosphorylation		Uniprot
T627	Phosphorylation	P11802 (CDK4) , Q00534 (CDK6)	Uniprot
S638	Phosphorylation		Uniprot
S672	Phosphorylation		Uniprot
S692	Phosphorylation		Uniprot
S693	Phosphorylation		Uniprot
S704	Phosphorylation	Q00534 (CDK6) , P11802 (CDK4)	Uniprot
S717	Phosphorylation		Uniprot
T719	Phosphorylation		Uniprot
S730	Phosphorylation	P53350 (PLK1)	Uniprot
S739	Phosphorylation	P53350 (PLK1)	Uniprot

Research Backgrounds

Q08050_FOXM1_HUMAN

Function:

Transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response.

PTMs:

Phosphorylated in M (mitotic) phase. Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair. Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates FOXM1, leading to activation of transcriptional activity and subsequent enhanced expression of key mitotic regulators.

Subcellular Location:

Nucleus.

Tissue Specificity:

Family&Domains:

Within the protein there is a domain which acts as a transcriptional activator. Insertion of a splicing sequence within it inactivates this transcriptional activity, as it is the case for isoform 4.

Research Fields

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

References

1). Copper overload impairs hematopoietic stem and progenitor cell proliferation via prompting HSF1/SP1 aggregation and the subsequently downregulating FOXM1-Cytoskeleton axis. iScience, 2023 (PubMed: 37009226) [IF=5.8]

Application: WB Species: zebrafish Sample: HSPCs

Figure 5 Dysfunction of gene foxm1 impairs the proliferation of HSPCs (A) Mitotic malformation of HSPCs in foxm1 morphants at 33 hpf (AGM) and 72 hpf (CHT), respectively. A1, A5, A9, A13, DAPI staining; A2, A6, A10, A14, anti-α-tubulin staining; A3, A7, A11, A15, anti-GFP staining; A4, A8, A12, A16, merged. At least 10 mitotic HSPCs in more than 10 embryos were observed for each group. A17, A18, calculation the length of spindles in metaphase runx1GFP+ cells at 33 hpf and 72 hpf, respectively. (B) BrdU cell proliferation in embryos at 33 hpf (B2-B9) and 72 hpf (B12-B19), respectively. B1, B11, AGM and CHT domain in embryos, respectively; B10, B20, calculation of HSPC proliferation in embryos from different groups. (C) The protein level of Foxm1 in foxm1 mutants with a 10-bp deletion at 33 hpf. GAPDH was used as an internal control. (D) Phenotypes of foxm1 mutants at 33 hpf and 120 hpf, respectively. (E) Expression of runx1 and cmyb in tuba1a mutants with or without Cu stresses at 33 hpf and 72 hpf. E9, E18, calculation of runx1 and cmyb expression in embryos from different groups. (F) Expression of tuba1a and stmn4 in foxm1 mutants with or without Cu stresses at 33 hpf. F9, F10, calculation of tuba1a and stmn4 expression in embryos from different groups. B2-B9, B12-B19, D1-D4, E1-E8, E10-E17, F1-F8, lateral view, anterior to the left, and dorsal to the up. Data are mean ± SD. t-test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, NS, not significant. Scale bars, 2 μm (A1-A16), 20 μm (B2-B9, B12-B19), 50 μm (E1-E8, F1-F8), 100 μm (D1-D4) and 200 μm (E10-E17).

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FOXM1 Antibody - #AF7860