Peroxiredoxin 1 Antibody | Affinity Biosciences

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Western blot analysis of Prdx1 in lysates of A431, using Prdx1 Antibody(AF7858).

Product:	Peroxiredoxin 1 Antibody
Catalog:	AF7858
Description:	Rabbit polyclonal antibody to Peroxiredoxin 1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	22kDa; 22kD(Calculated).
Uniprot:	Q06830
RRID:	AB_2844222

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Peroxiredoxin 1 Antibody detects endogenous levels of total Peroxiredoxin 1.

RRID:

AB_2844222
Cite Format: Affinity Biosciences Cat# AF7858, RRID:AB_2844222.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Heme binding 23 kDa protein; MSP23; Natural killer cell-enhancing factor A; NKEF A; NKEF-A; NKEFA; OSF3; Osteoblast specific factor 3; PAG; Paga; PAGB; Peroxiredoxin-1; PRDX1; PRDX1_HUMAN; Proliferation associated gene A; Proliferation-associated gene protein; PRX1; PrxI; TDPX2; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2;

Immunogens

Immunogen:

Uniprot:

Q06830(PRDX1_HUMAN) >>Visit HPA database.

Gene(ID):

PRDX1(5052)

Sequence:

Q06830 PRDX1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSGNAKIGHPAPNFKATAVMPDGQFKDISLSDYKGKYVVFFFYPLDFTFVCPTEIIAFSDRAEEFKKLNCQVIGASVDSHFCHLAWVNTPKKQGGLGPMNIPLVSDPKRTIAQDYGVLKADEGISFRGLFIIDDKGILRQITVNDLPVGRSVDETLRLVQAFQFTDKHGEVCPAGWKPGSDTIKPDVQKSKEYFSKQK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q06830 As Substrate

Q06830

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
K7	Acetylation		Uniprot
K7	Sumoylation		Uniprot
K7	Ubiquitination		Uniprot
K16	Acetylation		Uniprot
K16	Methylation		Uniprot
K16	Sumoylation		Uniprot
K16	Ubiquitination		Uniprot
T18	Phosphorylation	Q13043 (STK4)	Uniprot
K27	Acetylation		Uniprot
K27	Methylation		Uniprot
K27	Ubiquitination		Uniprot
S30	Phosphorylation		Uniprot
S32	Phosphorylation	Q96KB5 (PBK)	Uniprot
Y34	Phosphorylation		Uniprot
K35	Acetylation		Uniprot
K35	Ubiquitination		Uniprot
K37	Acetylation		Uniprot
K37	Ubiquitination		Uniprot
C52	S-Nitrosylation		Uniprot
K67	Acetylation		Uniprot
K67	Sumoylation		Uniprot
K68	Ubiquitination		Uniprot
S77	Phosphorylation		Uniprot
S80	Phosphorylation		Uniprot
T90	Phosphorylation	P24941 (CDK2) , Q00534 (CDK6) , P06493 (CDK1) , P11802 (CDK4) , Q13043 (STK4)	Uniprot
K92	Acetylation		Uniprot
K93	Acetylation		Uniprot
K93	Sumoylation		Uniprot
K93	Ubiquitination		Uniprot
S106	Phosphorylation		Uniprot
K109	Acetylation		Uniprot
K109	Ubiquitination		Uniprot
T111	Phosphorylation		Uniprot
Y116	Phosphorylation		Uniprot
K120	Ubiquitination		Uniprot
S126	Phosphorylation		Uniprot
K136	Acetylation		Uniprot
K136	Ubiquitination		Uniprot
T143	Phosphorylation		Uniprot
R151	Methylation		Uniprot
S152	Phosphorylation		Uniprot
T156	Phosphorylation		Uniprot
R158	Methylation		Uniprot
T166	Phosphorylation		Uniprot
K168	Acetylation		Uniprot
K168	Ubiquitination		Uniprot
C173	S-Nitrosylation		Uniprot
K178	Acetylation		Uniprot
K178	Methylation		Uniprot
K178	Ubiquitination		Uniprot
S181	Phosphorylation		Uniprot
T183	Phosphorylation	Q13043 (STK4)	Uniprot
K185	Acetylation		Uniprot
K185	Sumoylation		Uniprot
K185	Ubiquitination		Uniprot
K190	Ubiquitination		Uniprot
K192	Sumoylation		Uniprot
K192	Ubiquitination		Uniprot
Y194	Phosphorylation	P06239 (LCK)	Uniprot
S196	Phosphorylation		Uniprot
K197	Acetylation		Uniprot
K197	Ubiquitination		Uniprot

Research Backgrounds

Q06830_PRDX1_HUMAN

Function:

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).

PTMs:

Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.

Subcellular Location:

Cytoplasm. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Subunit Structure:

Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer. Interacts with GDPD5; forms a mixed-disulfide with GDPD5 (By similarity). Interacts with SESN1 and SESN2. Interacts with FAM107A.

Family&Domains:

Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

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Peroxiredoxin 1 Antibody - #AF7858