Product: MEF2C Antibody
Catalog: AF7856
Description: Rabbit polyclonal antibody to MEF2C
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 51.2kDa; 51kD(Calculated).
Uniprot: Q06413
RRID: AB_2844220

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MEF2C Antibody detects endogenous levels of total MEF2C.
RRID:
AB_2844220
Cite Format: Affinity Biosciences Cat# AF7856, RRID:AB_2844220.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C5DELq14.3; DEL5q14.3; MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C); MADS box transcription enhancer factor 2, polypeptide C; MEF2C; MEF2C_HUMAN; Myocyte enhancer factor 2C; Myocyte specific enhancer factor 2C; Myocyte-specific enhancer factor 2C; OTTHUMP00000222409; Similar to MADS box transcription enhancer factor 2 polypeptide C;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q06413 MEF2C_HUMAN:

Expressed in brain and skeletal muscle.

Sequence:
MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSTNKLFQYASTDMDKVLLKYTEYNEPHESRTNSDIVETLRKKGLNGCDSPDPDADDSVGHSPESEDKYRKINEDIDLMISRQRLCAVPPPNFEMPVSIPVSSHNSLVYSNPVSSLGNPNLLPLAHPSLQRNSMSPGVTHRPPSAGNTGGLMGGDLTSGAGTSAGNGYGNPRNSPGLLVSPGNLNKNMQAKSPPPMNLGMNNRKPDLRVLIPPGSKNTMPSVSEDVDLLLNQRINNSQSAQSLATPVVSVATPTLPGQGMGGYPSAISTTYGTEYSLSSADLSSLSGFNTASALHLGSVTGWQQQHLHNMPPSALSQLGACTSTHLSQSSNLSLPSTQSLNIKSEPVSPPRDRTTTPSRYPQHTRHEAGRSPVDSLSSCSSSYDGSDREDHRNEFHSPIGLTRPSPDERESPSVKRMRLSEGWAT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Bovine
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q06413 As Substrate

Site PTM Type Enzyme
T20 Phosphorylation
K23 Ubiquitination
S59 Phosphorylation
K64 Ubiquitination
K68 Ubiquitination
T80 Phosphorylation Q9H1R3 (MYLK2)
S82 Phosphorylation
T87 Phosphorylation
S98 Phosphorylation
S106 Phosphorylation
S110 Phosphorylation
K116 Acetylation
K119 Acetylation
S181 Phosphorylation
S183 Phosphorylation
S192 Phosphorylation P51812 (RPS6KA3)
S222 Phosphorylation
S228 Phosphorylation
K234 Acetylation
K239 Acetylation
S240 Phosphorylation
K252 Acetylation
K264 Acetylation
T293 Phosphorylation Q16539 (MAPK14)
T300 Phosphorylation Q16539 (MAPK14)
S387 Phosphorylation Q13164 (MAPK7) , Q16539 (MAPK14)
T387 Phosphorylation
K391 Sumoylation
S396 Phosphorylation P06493 (CDK1)
S419 Phosphorylation
S445 Phosphorylation
S453 Phosphorylation
S459 Phosphorylation
S461 Phosphorylation

Research Backgrounds

Function:

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoforms that lack the repressor domain are more active than isoform 1.

PTMs:

Phosphorylation on Ser-59 enhances DNA binding activity (By similarity). Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity.

Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity).

Sumoylated on Lys-391 with SUMO2 but not by SUMO1 represses transcriptional activity.

Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.

Subcellular Location:

Nucleus. Cytoplasm>Sarcoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in brain and skeletal muscle.

Subunit Structure:

Forms a complex with class II HDACs in undifferentiating cells. On myogenic differentiation, HDACs are released into the cytoplasm allowing MEF2s to interact with other proteins for activation. Interacts with EP300 in differentiating cells; the interaction acetylates MEF2C leading to increased DNA binding and activation (By similarity). Interacts with HDAC7 and CARM1 (By similarity). Interacts with HDAC4 and HDAC9; the interaction with HDACs represses transcriptional activity. Interacts with LPIN1. Interacts with MYOCD. Interacts with AKAP13 (By similarity). Interacts with FOXK1; the interaction inhibits MEF2C transactivation activity (By similarity). Interacts (via N-terminus) with HABP4; this interaction decreases DNA-binding activity of MEF2C in myocardial cells in response to mechanical stress. Interacts with JPH2; interaction specifically takes place with the Junctophilin-2 N-terminal fragment cleavage product of JPH2 (By similarity). Interacts (via MADS box) with SOX18 (By similarity).

Family&Domains:

The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.

Belongs to the MEF2 family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

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