Phospho-SREBP1 (Ser439) Antibody | Affinity Biosciences

Product:	Phospho-SREBP1 (Ser439) Antibody
Catalog:	AF3283
Description:	Rabbit polyclonal antibody to Phospho-SREBP1 (Ser439)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Sheep, Dog
Mol.Wt.:	122kD, 65kD(cleaved); 122kD(Calculated).
Uniprot:	P36956
RRID:	AB_2834704

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Phospho-SREBP1 (Ser439) Antibody detects endogenous levels of SREBP1 only when phosphorylated at Serine 439.

RRID:

AB_2834704
Cite Format: Affinity Biosciences Cat# AF3283, RRID:AB_2834704.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ADD 1; bHLHd1; Class D basic helix-loop-helix protein 1; D630008H06; Processed sterol regulatory element-binding protein 1; SRBP1_HUMAN; SREBF 1; SREBF1; SREBP 1; SREBP 1c; SREBP-1; SREBP1; Sterol regulatory element binding protein 1; Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1; Sterol regulatory element binding transcription factor 1; Sterol regulatory element-binding transcription factor 1;

Immunogens

Immunogen:

Uniprot:

P36956(SRBP1_HUMAN) >>Visit HPA database.

Gene(ID):

SREBF1(6720)

Expression:

P36956 SRBP1_HUMAN:

Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.

Description:

This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum.

Sequence:

P36956 SRBP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDEPPFSEAALEQALGEPCDLDAALLTDIEDMLQLINNQDSDFPGLFDPPYAGSGAGGTDPASPDTSSPGSLSPPPATLSSSLEAFLSGPQAAPSPLSPPQPAPTPLKMYPSMPAFSPGPGIKEESVPLSILQTPTPQPLPGALLPQSFPAPAPPQFSSTPVLGYPSPPGGFSTGSPPGNTQQPLPGLPLASPPGVPPVSLHTQVQSVVPQQLLTVTAAPTAAPVTTTVTSQIQQVPVLLQPHFIKADSLLLTAMKTDGATVKAAGLSPLVSGTTVQTGPLPTLVSGGTILATVPLVVDAEKLPINRLAAGSKAPASAQSRGEKRTAHNAIEKRYRSSINDKIIELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLSLRTAVHKSKSLKDLVSACGSGGNTDVLMEGVKTEVEDTLTPPPSDAGSPFQSSPLSLGSRGSGSGGSGSDSEPDSPVFEDSKAKPEQRPSLHSRGMLDRSRLALCTLVFLCLSCNPLASLLGARGLPSPSDTTSVYHSPGRNVLGTESRDGPGWAQWLLPPVVWLLNGLLVLVSLVLLFVYGEPVTRPHSGPAVYFWRHRKQADLDLARGDFAQAAQQLWLALRALGRPLPTSHLDLACSLLWNLIRHLLQRLWVGRWLAGRAGGLQQDCALRVDASASARDAALVYHKLHQLHTMGKHTGGHLTATNLALSALNLAECAGDAVSVATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGSVPPAMQWLCHPVGHRFFVDGDWSVLSTPWESLYSLAGNPVDPLAQVTQLFREHLLERALNCVTQPNPSPGSADGDKEFSDALGYLQLLNSCSDAAGAPAYSFSISSSMATTTGVDPVAKWWASLTAVVIHWLRRDEEAAERLCPLVEHLPRVLQESERPLPRAALHSFKAARALLGCAKAESGPASLTICEKASGYLQDSLATTPASSSIDKAVQLFLCDLLLVVRTSLWRQQQPPAPAPAAQGTSSRPQASALELRGFQRDLSSLRRLAQSFRPAMRRVFLHEATARLMAGASPTRTHQLLDRSLRRRAGPGGKGGAVAELEPRPTRREHAEALLLASCYLPPGFLSAPGQRVGMLAEAARTLEKLGDRRLLHDCQQMLMRLGGGTTVTSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Bovine

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P36956 As Substrate

P36956

Site	PTM Type	Enzyme	Source
T59	Phosphorylation		Uniprot
S63	Phosphorylation	Q16539 (MAPK14)	Uniprot
S117	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1) , P45983 (MAPK8)	Uniprot
K123	Sumoylation		Uniprot
K256	Ubiquitination		Uniprot
T275	Phosphorylation		Uniprot
S312	Phosphorylation		Uniprot
K313	Ubiquitination		Uniprot
R321	Methylation		Uniprot
K324	Acetylation		Uniprot
K324	Ubiquitination		Uniprot
K333	Acetylation		Uniprot
S337	Phosphorylation	P57059 (SIK1)	Uniprot
S338	Phosphorylation	P57059 (SIK1) , P17612 (PRKACA)	Uniprot
K342	Ubiquitination		Uniprot
K347	Ubiquitination		Uniprot
K356	Ubiquitination		Uniprot
K359	Ubiquitination		Uniprot
S360	Phosphorylation		Uniprot
K365	Ubiquitination		Uniprot
K379	Ubiquitination		Uniprot
K381	Ubiquitination		Uniprot
S396	Phosphorylation	P54646 (PRKAA2)	Uniprot
K398	Ubiquitination		Uniprot
S402	Phosphorylation	P57059 (SIK1)	Uniprot
K418	Sumoylation		Uniprot
T424	Phosphorylation		Uniprot
T426	Phosphorylation	P49841 (GSK3B) , Q16539 (MAPK14)	Uniprot
S430	Phosphorylation	P49841 (GSK3B)	Uniprot
S434	Phosphorylation	P49841 (GSK3B)	Uniprot
S439	Phosphorylation	P06493 (CDK1)	Uniprot
S448	Phosphorylation		Uniprot
S450	Phosphorylation		Uniprot
S455	Phosphorylation		Uniprot
S457	Phosphorylation		Uniprot
S467	Phosphorylation	P53350 (PLK1)	Uniprot
K470	Ubiquitination		Uniprot
S486	Phosphorylation		Uniprot
Y567	Phosphorylation		Uniprot
K587	Ubiquitination		Uniprot
K675	Ubiquitination		Uniprot
K727	Ubiquitination		Uniprot
K924	Methylation		Uniprot
K924	Ubiquitination		Uniprot
K934	Ubiquitination		Uniprot
K947	Ubiquitination		Uniprot
S1020	Phosphorylation		Uniprot
S1027	Phosphorylation		Uniprot
S1049	Phosphorylation		Uniprot
S1060	Phosphorylation		Uniprot
K1070	Ubiquitination		Uniprot
K1121	Ubiquitination		Uniprot

Research Backgrounds

P36956_SRBP1_HUMAN

Function:

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').

PTMs:

At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.

Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding (By similarity).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein.
Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.

Nucleus.

Nucleus.

Nucleus.

Tissue Specificity:

Subunit Structure:

Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Interacts with CEBPA, the interaction produces a transcriptional synergy (By similarity).

Family&Domains:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the SREBP family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

References

1). LPCAT1 reprogramming cholesterol metabolism promotes the progression of esophageal squamous cell carcinoma. Cell death & disease, 2021 (PubMed: 34518524) [IF=9.0]

Application: WB Species: Human Sample: EC9706 and TE1 cells

Fig. 5 LPCAT1 promote ESCC cholesterol synthesis by EGFR/INSIG-1/SREBP-1 pathway. A The level of cholesterol in EC9706 and TE1 cells transfected with sh-control and sh-LPCAT1 was detected using the cholesterol detection kit. B The expression of SQLE, Insig-1, and MSMO1 in EC9706 and TE1 cells transfected with sh-control and sh-LPCAT1 was detected using western blot. C, D The expression of p-SREBP-1 in the nucleus in EC9706 and TE1 cells transfected with sh-control and sh-LPCAT1 was detected using western blot and immunofluorescence. E The expression of p-EGFR and p-PI3K in EC9706 and TE1 cells transfected with sh-control and sh-LPCAT1 was detected using western blot. F The expression of SQLE, Insig-1, and MSMO1 in EC9706 and TE1 cells treated with NSC228155 or 740Y-P after transfection with sh-LPCAT1 was detected using western blot. G The expression of p-SREBP-1 in the nucleus in EC9706 and TE1 cells treated with NSC228155 or 740Y-P after transfection with or without sh-LPCAT1 was detected using western blot. H The level of cholesterol in EC9706 and TE1 cells treated with NSC228155 or 740Y-P after transfection with or without sh-LPCAT1 was detected using the cholesterol detection kit. Data represent three independent experiments. *P

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Phospho-SREBP1 (Ser439) Antibody - #AF3283