SHP1 Antibody - #AF7787
Product: | SHP1 Antibody |
Catalog: | AF7787 |
Description: | Rabbit polyclonal antibody to SHP1 |
Application: | WB |
Reactivity: | Human, Mouse, Rat |
Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
Mol.Wt.: | 67kDa; 68kD(Calculated). |
Uniprot: | P29350 |
RRID: | AB_2844151 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF7787, RRID:AB_2844151.
Fold/Unfold
70Z-SHP; EC 3.1.3.48; HCP; HCPH; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP1C; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase non receptor type 6; Protein tyrosine phosphatase SHP1; Protein-tyrosine phosphatase 1C; protein-tyrosine phosphatase SHP 1; Protein-tyrosine phosphatase SHP-1; PTN6_HUMAN; PTP 1C; PTP-1C; PTP1C; Ptpn6; SH PTP 1; SH PTP1; SH-PTP1; SHP 1; SHP 1L; SHP1; SHP1L; tyrosine protein phosphatase non receptor type 6; Tyrosine-protein phosphatase non-receptor type 6;
Immunogens
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
- P29350 PTN6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVRWFHRDLSGLDAETLLKGRGVHGSFLARPSRKNQGDFSLSVRVGDQVTHIRIQNSGDFYDLYGGEKFATLTELVEYYTQQQGVLQDRDGTIIHLKYPLNCSDPTSERWYHGHMSGGQAETLLQAKGEPWTFLVRESLSQPGDFVLSVLSDQPKAGPGSPLRVTHIKVMCEGGRYTVGGLETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKGKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYIKNQLLGPDENAKTYIASQGCLEATVNDFWQMAWQENSRVIVMTTREVEKGRNKCVPYWPEVGMQRAYGPYSVTNCGEHDTTEYKLRTLQVSPLDNGDLIREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMENISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P29350 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S10 | Phosphorylation | Uniprot | |
K19 | Ubiquitination | Uniprot | |
S26 | Phosphorylation | Uniprot | |
K34 | Ubiquitination | Uniprot | |
S57 | Phosphorylation | Uniprot | |
Y61 | Phosphorylation | Uniprot | |
Y64 | Phosphorylation | Uniprot | |
K97 | Ubiquitination | Uniprot | |
Y98 | Phosphorylation | Uniprot | |
Y111 | Phosphorylation | Uniprot | |
S140 | Phosphorylation | Uniprot | |
K155 | Ubiquitination | Uniprot | |
K168 | Ubiquitination | Uniprot | |
Y176 | Phosphorylation | Uniprot | |
T177 | Phosphorylation | Uniprot | |
S186 | Phosphorylation | Uniprot | |
T188 | Phosphorylation | Uniprot | |
K195 | Ubiquitination | Uniprot | |
K196 | Ubiquitination | Uniprot | |
Y208 | Phosphorylation | Uniprot | |
Y213 | Phosphorylation | Uniprot | |
Y214 | Phosphorylation | Uniprot | |
K232 | Ubiquitination | Uniprot | |
K241 | Ubiquitination | Uniprot | |
S250 | Phosphorylation | Uniprot | |
K253 | Ubiquitination | Uniprot | |
K257 | Ubiquitination | Uniprot | |
K271 | Ubiquitination | Uniprot | |
Y276 | Phosphorylation | Uniprot | |
K277 | Acetylation | Uniprot | |
K277 | Ubiquitination | Uniprot | |
S285 | Phosphorylation | Uniprot | |
R286 | Methylation | Uniprot | |
S299 | Phosphorylation | Uniprot | |
Y301 | Phosphorylation | Uniprot | |
Y306 | Phosphorylation | Uniprot | |
K308 | Ubiquitination | Uniprot | |
K360 | Ubiquitination | Uniprot | |
Y364 | Phosphorylation | Uniprot | |
Y374 | Phosphorylation | Uniprot | |
Y377 | Phosphorylation | Uniprot | |
S378 | Phosphorylation | Uniprot | |
K391 | Ubiquitination | Uniprot | |
T394 | Phosphorylation | Uniprot | |
S398 | Phosphorylation | Uniprot | |
C453 | S-Nitrosylation | Uniprot | |
K486 | Ubiquitination | Uniprot | |
S496 | Phosphorylation | Uniprot | |
Y509 | Phosphorylation | Uniprot | |
K521 | Ubiquitination | Uniprot | |
K522 | Ubiquitination | Uniprot | |
S528 | Phosphorylation | Uniprot | |
K530 | Ubiquitination | Uniprot | |
S534 | Phosphorylation | Uniprot | |
Y536 | Phosphorylation | P06213 (INSR) , P00519 (ABL1) , P12931 (SRC) , A0A173G4P4 (Abl fusion) , P06239 (LCK) , P07948 (LYN) | Uniprot |
T540 | Phosphorylation | Uniprot | |
Y541 | Phosphorylation | Uniprot | |
K546 | Ubiquitination | Uniprot | |
S553 | Phosphorylation | Uniprot | |
T555 | Phosphorylation | Uniprot | |
S556 | Phosphorylation | Uniprot | |
S557 | Phosphorylation | Uniprot | |
K560 | Ubiquitination | Uniprot | |
Y564 | Phosphorylation | A0A173G4P4 (Abl fusion) , P00519 (ABL1) , P07948 (LYN) , P06239 (LCK) , P12931 (SRC) | Uniprot |
K570 | Ubiquitination | Uniprot | |
S582 | Phosphorylation | Uniprot | |
S588 | Phosphorylation | Uniprot | |
S591 | Phosphorylation | P17252 (PRKCA) , P24941 (CDK2) | Uniprot |
Research Backgrounds
Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation (By similarity). Phosphorylation at Tyr-564 enhances phosphatase activity.
Cytoplasm. Nucleus.
Note: In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). Shuttles between the cytoplasm and nucleus via its association with PDPK1.
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Monomer. Interacts with MTUS1 (By similarity). Interacts with MILR1 (tyrosine-phosphorylated) (By similarity). Interacts with KIT (By similarity). Interacts with SIRPA/PTPNS1. Interacts with LILRB1 and LILRB2. Interacts with FCRL2 and FCRL4. Interacts with FCRL3 and FCRL6 (tyrosine phosphorylated form). Interacts with CD84. Interacts with CD300LF. Interacts with CDK2. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1. Interacts with CEACAM1 (via cytoplasmic domain); this interaction depends on the monomer/dimer equilibrium and is phosphorylation-dependent (By similarity). Interacts with MPIG6B (via ITIM motif). Interacts with moesin/MSN.
The N-terminal SH2 domain functions as an auto-inhibitory domain, blocking the catalytic domain in the ligand-free close conformation.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
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