Phospholamban Antibody - #AF7778

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Product: Phospholamban Antibody
Catalog: AF7778
Description: Rabbit polyclonal antibody to Phospholamban
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 24kDa; 6kD(Calculated).
Uniprot: P26678
RRID: AB_2844142

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 100ul $350 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospholamban Antibody detects endogenous levels of total Phospholamban.
RRID:
AB_2844142
Cite Format: Affinity Biosciences Cat# AF7778, RRID:AB_2844142.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cardiac phospholamban; CMD1P; CMH18; PLB; Pln; PPLA_HUMAN;

Immunogens

Immunogen:
Uniprot:

P26678(PPLA_HUMAN) >>Visit HPA database.

Gene(ID):
PLN(5350)
Expression:
P26678 PPLA_HUMAN:

Heart muscle (at protein level).

Sequence:
MEKVQYLTRSAIRRASTIEMPQQARQKLQNLFINFCLILICLLLICIIVMLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
67
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P26678 As Substrate

Site PTM Type Enzyme
S16 Phosphorylation Q09013 (DMPK) , P17612 (PRKACA) , P31749 (AKT1)
T17 Phosphorylation Q13557 (CAMK2D) , Q9UQM7 (CAMK2A) , Q13554 (CAMK2B)

Research Backgrounds

Function:

Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.

PTMs:

Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.

Palmitoylated by ZDHHC16, promoting formation of the homopentamer.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Sarcoplasmic reticulum membrane>Single-pass membrane protein. Mitochondrion membrane>Single-pass membrane protein. Membrane>Single-pass membrane protein.
Note: Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Heart muscle (at protein level).

Subunit Structure:

Homopentamer. Interacts with HAX1 and ATP2A2.

Family&Domains:

Belongs to the phospholamban family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

References

1). Over-expression of Kv4. 3 gene reverses cardiac remodeling and transient-outward K+ current (Ito) reduction via CaMKII inhibition in myocardial infarction. Biomedicine & Pharmacotherapy, 2020 (PubMed: 33254430) [IF=7.5]

Application: WB    Species: rat    Sample: Heart

Fig. 6. Overexpression of Kv4.3 reversed the activation of CaMKII induced by MI in vivo. (A) Representative proteins of phospho-PLB(Thr17), PLB, phospho-CaMKII, CaMKII, phospho-RYR2(ser 2814), RYR2, and SERCA2 detected by western blot. (B–H) Quantitative results of phospho-PLB(Thr17), PLB, phospho-CaMKII, CaMKII, phospho-RYR2(ser 2814), RYR2, and SERCA2 in the indicated groups (n = 3). (a) SO group, (b) MI group, (c) AD-EGFP group, (d) AD-Kv4.3 group, (e) M + C group. Data are shown as mean ± SEM.
2). A-kinase Anchoring Protein 5 Anchors Protein Kinase A to Mediate PLN/SERCA to Reduce Cardiomyocyte Apoptosis Induced by Hypoxia and Reoxygenation. Biochemistry and Cell Biology, 2022 (PubMed: 35041539) [IF=2.9]

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