eIF4B Antibody | Affinity Biosciences

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Western blot analysis of eIF4B in lysates of NIH-3T3, using eIF4B Antibody(AF7768).

Product:	eIF4B Antibody
Catalog:	AF7768
Description:	Rabbit polyclonal antibody to eIF4B
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	80kDa; 69kD(Calculated).
Uniprot:	P23588
RRID:	AB_2844132

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

eIF4B Antibody detects endogenous levels of total eIF4B.

RRID:

AB_2844132
Cite Format: Affinity Biosciences Cat# AF7768, RRID:AB_2844132.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2310046H11Rik; AL024095; C85189; EIF 4B; eIF-4B; EIF4B; Eukaryotic initiation factor 4B; Eukaryotic translation initiation factor 4B; IF4B_HUMAN; PRO1843;

Immunogens

Immunogen:

Uniprot:

P23588(IF4B_HUMAN) >>Visit HPA database.

Gene(ID):

EIF4B(1975)

Sequence:

P23588 IF4B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVSKPVSWADETDDLEGDVSTTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFGYAEFEDLDSLLSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWRARPATDSFDDYPPRRGDDSFGDKYRDRYDSDRYRDGYRDGYRDGPRRDMDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPKLNLKPRSTPKEDDSSASTSQSTRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLERRPRERHPSWRSEETQERERSRTGSESSQTGTSTTSSRNARRRESEKSLENETLNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDTEQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQTGNSSRGPGDGGNRDHWKESDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDGEDENEGEDYAE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Pig

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23588 As Substrate

P23588

Site	PTM Type	Enzyme	Source
K6	Acetylation		Uniprot
S16	Phosphorylation		Uniprot
Y33	Phosphorylation		Uniprot
S39	Phosphorylation		Uniprot
S52	Phosphorylation		Uniprot
S57	Phosphorylation		Uniprot
Y63	Phosphorylation		Uniprot
R70	Methylation		Uniprot
S71	Phosphorylation		Uniprot
T75	Phosphorylation		Uniprot
R78	Methylation		Uniprot
K92	Ubiquitination		Uniprot
S93	Phosphorylation		Uniprot
Y96	Phosphorylation		Uniprot
T97	Phosphorylation		Uniprot
Y105	Phosphorylation		Uniprot
S111	Phosphorylation		Uniprot
R117	Methylation		Uniprot
S131	Phosphorylation		Uniprot
Y141	Phosphorylation		Uniprot
S155	Phosphorylation		Uniprot
S160	Phosphorylation		Uniprot
K177	Ubiquitination		Uniprot
R182	Methylation		Uniprot
S183	Phosphorylation		Uniprot
S192	Phosphorylation		Uniprot
T195	Phosphorylation		Uniprot
T197	Phosphorylation		Uniprot
T205	Phosphorylation		Uniprot
S207	Phosphorylation		Uniprot
Y211	Phosphorylation		Uniprot
S219	Phosphorylation		Uniprot
K223	Ubiquitination		Uniprot
Y224	Phosphorylation		Uniprot
Y228	Phosphorylation		Uniprot
S230	Phosphorylation		Uniprot
Y233	Phosphorylation		Uniprot
Y237	Phosphorylation		Uniprot
Y241	Phosphorylation		Uniprot
Y252	Phosphorylation		Uniprot
Y258	Phosphorylation		Uniprot
R261	Methylation		Uniprot
S263	Phosphorylation		Uniprot
Y266	Phosphorylation		Uniprot
R268	Methylation		Uniprot
Y270	Phosphorylation		Uniprot
S272	Phosphorylation		Uniprot
R273	Methylation		Uniprot
R279	Methylation		Uniprot
S283	Phosphorylation		Uniprot
Y285	Phosphorylation		Uniprot
Y291	Phosphorylation		Uniprot
R292	Methylation		Uniprot
R297	Methylation		Uniprot
Y298	Phosphorylation		Uniprot
Y302	Phosphorylation		Uniprot
R308	Methylation		Uniprot
S309	Phosphorylation		Uniprot
S311	Phosphorylation		Uniprot
S312	Phosphorylation		Uniprot
Y316	Phosphorylation		Uniprot
S317	Phosphorylation		Uniprot
Y321	Phosphorylation		Uniprot
K333	Sumoylation		Uniprot
S340	Phosphorylation		Uniprot
T341	Phosphorylation		Uniprot
S347	Phosphorylation		Uniprot
S348	Phosphorylation		Uniprot
S350	Phosphorylation		Uniprot
T351	Phosphorylation		Uniprot
S352	Phosphorylation		Uniprot
S354	Phosphorylation		Uniprot
S359	Phosphorylation		Uniprot
K365	Acetylation		Uniprot
K365	Ubiquitination		Uniprot
T369	Phosphorylation		Uniprot
S406	Phosphorylation	Q9P1W9 (PIM2) , Q14680 (MELK)	Uniprot
S409	Phosphorylation		Uniprot
T412	Phosphorylation		Uniprot
S418	Phosphorylation		Uniprot
T420	Phosphorylation		Uniprot
S422	Phosphorylation	P51812 (RPS6KA3) , Q15418 (RPS6KA1) , P23443 (RPS6KB1) , P31749 (AKT1)	Uniprot
S424	Phosphorylation		Uniprot
S425	Phosphorylation		Uniprot
T427	Phosphorylation		Uniprot
T429	Phosphorylation		Uniprot
S430	Phosphorylation		Uniprot
T431	Phosphorylation		Uniprot
T432	Phosphorylation		Uniprot
S433	Phosphorylation		Uniprot
S434	Phosphorylation		Uniprot
S442	Phosphorylation		Uniprot
K444	Acetylation		Uniprot
S445	Phosphorylation		Uniprot
T450	Phosphorylation		Uniprot
S459	Phosphorylation		Uniprot
T461	Phosphorylation		Uniprot
S462	Phosphorylation		Uniprot
K463	Ubiquitination		Uniprot
K486	Methylation		Uniprot
S488	Phosphorylation		Uniprot
S489	Phosphorylation		Uniprot
S495	Phosphorylation		Uniprot
S497	Phosphorylation		Uniprot
S498	Phosphorylation		Uniprot
T500	Phosphorylation		Uniprot
S504	Phosphorylation		Uniprot
T506	Phosphorylation		Uniprot
S518	Phosphorylation		Uniprot
K537	Ubiquitination		Uniprot
S585	Phosphorylation		Uniprot
K586	Acetylation		Uniprot
K586	Ubiquitination		Uniprot
S589	Phosphorylation		Uniprot
S591	Phosphorylation		Uniprot
K592	Ubiquitination		Uniprot
Y593	Phosphorylation		Uniprot
S597	Phosphorylation		Uniprot
Y609	Phosphorylation		Uniprot

Research Backgrounds

P23588_IF4B_HUMAN

Function:

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

PTMs:

Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.

Subunit Structure:

Self-associates and interacts with EIF3 p170 subunit.

Research Fields

· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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eIF4B Antibody - #AF7768