Cofilin Antibody - #AF7766
| Product: | Cofilin Antibody |
| Catalog: | AF7766 |
| Description: | Rabbit polyclonal antibody to Cofilin |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 19kDa; 19kD(Calculated). |
| Uniprot: | P23528 |
| RRID: | AB_2844130 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF7766, RRID:AB_2844130.
Fold/Unfold
18 kDa phosphoprotein; CFL 1; CFL; CFL1; COF1_HUMAN; Cofilin 1; Cofilin 1 non muscle; Cofilin; Cofilin non muscle isoform; Cofilin-1; epididymis secretory protein Li 15; HEL-S-15; non-muscle isoform; p18;
Immunogens
- P23528 COF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P23528 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S3 | Phosphorylation | P53671 (LIMK2) , P53667 (LIMK1) , Q13418 (ILK) , Q15139 (PRKD1) , Q96S53 (TESK2) , Q15569 (TESK1) , Q7Z2Y5 (NRK) | Uniprot |
| S8 | Phosphorylation | Uniprot | |
| K13 | Acetylation | Uniprot | |
| K13 | Sumoylation | Uniprot | |
| K13 | Ubiquitination | Uniprot | |
| K19 | Acetylation | Uniprot | |
| K19 | Methylation | Uniprot | |
| K19 | Sumoylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| S23 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S24 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T25 | Phosphorylation | Uniprot | |
| K30 | Acetylation | Uniprot | |
| K30 | Sumoylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K31 | Acetylation | Uniprot | |
| K33 | Acetylation | Uniprot | |
| K34 | Sumoylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| C39 | S-Nitrosylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| K44 | Acetylation | Uniprot | |
| K44 | Sumoylation | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| K45 | Acetylation | Uniprot | |
| K45 | Sumoylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K53 | Sumoylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| T63 | Phosphorylation | Uniprot | |
| Y68 | Phosphorylation | P12931 (SRC) | Uniprot |
| T70 | Phosphorylation | Uniprot | |
| K73 | Acetylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K78 | Acetylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| C80 | S-Nitrosylation | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| Y85 | Phosphorylation | Uniprot | |
| T88 | Phosphorylation | Uniprot | |
| Y89 | Phosphorylation | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| K92 | Acetylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K96 | Acetylation | Uniprot | |
| K96 | Methylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| K112 | Sumoylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K114 | Acetylation | Uniprot | |
| K114 | Sumoylation | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| Y117 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| K121 | Acetylation | Uniprot | |
| K121 | Sumoylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K125 | Acetylation | Uniprot | |
| K126 | Acetylation | Uniprot | |
| K127 | Acetylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| K132 | Acetylation | Uniprot | |
| K132 | Sumoylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| C139 | S-Nitrosylation | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| K144 | Acetylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| C147 | S-Nitrosylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| K164 | Sumoylation | Uniprot | |
| K164 | Ubiquitination | Uniprot |
Research Backgrounds
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration (By similarity).
Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2.
Nucleus matrix. Cytoplasm>Cytoskeleton. Cell projection>Ruffle membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Lamellipodium.
Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
Widely distributed in various tissues.
Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods.
Belongs to the actin-binding proteins ADF family.
Research Fields
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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