JunD Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Pc12, using JunD Antibody.

Product:	JunD Antibody
Catalog:	AF7744
Description:	Rabbit polyclonal antibody to JunD
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Dog, Chicken
Mol.Wt.:	38kDa, 50 kDa; 35kD(Calculated).
Uniprot:	P17535
RRID:	AB_2844108

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

JunD Antibody detects endogenous levels of total JunD.

RRID:

AB_2844108
Cite Format: Affinity Biosciences Cat# AF7744, RRID:AB_2844108.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Activator protein 1; AP 1; AP1; Jun D; jun D proto oncogene; Jund; JunD FL isoform; JUND_HUMAN; Transcription factor jun D; Transcription factor jun-D;

Immunogens

Immunogen:

Uniprot:

P17535(JUND_HUMAN) >>Visit HPA database.

Gene(ID):

JUND(3727)

Sequence:

P17535 JUND_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

METPFYGDEALSGLGGGASGSGGSFASPGRLFPGAPPTAAAGSMMKKDALTLSLSEQVAAALKPAAAPPPTPLRADGAPSAAPPDGLLASPDLGLLKLASPELERLIIQSNGLVTTTPTSSQFLYPKVAASEEQEFAEGFVKALEDLHKQNQLGAGAAAAAAAAAAGGPSGTATGSAPPGELAPAAAAPEAPVYANLSSYAGGAGGAGGAATVAFAAEPVPFPPPPPPGALGPPRLAALKDEPQTVPDVPSFGESPPLSPIDMDTQERIKAERKRLRNRIAASKCRKRKLERISRLEEKVKTLKSQNTELASTASLLREQVAQLKQKVLSHVNSGCQLLPQHQVPAY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Dog

100

Zebrafish

100

Chicken

100

Xenopus

Horse

Sheep

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17535 As Substrate

P17535

Site	PTM Type	Enzyme	Source
S27	Phosphorylation		Uniprot
T38	Phosphorylation		Uniprot
S43	Phosphorylation		Uniprot
T71	Phosphorylation		Uniprot
S90	Phosphorylation	P45983 (MAPK8)	Uniprot
K97	Ubiquitination		Uniprot
S100	Phosphorylation	P45983 (MAPK8) , P27361 (MAPK3) , P28482 (MAPK1)	Uniprot
T117	Phosphorylation	P45983 (MAPK8)	Uniprot
K127	Ubiquitination		Uniprot
S131	Phosphorylation		Uniprot
K142	Ubiquitination		Uniprot
K240	Sumoylation		Uniprot
T245	Phosphorylation		Uniprot
S251	Phosphorylation		Uniprot
S255	Phosphorylation		Uniprot
S259	Phosphorylation		Uniprot
T265	Phosphorylation		Uniprot
S294	Phosphorylation		Uniprot
S305	Phosphorylation		Uniprot
T308	Phosphorylation		Uniprot
S312	Phosphorylation		Uniprot
T313	Phosphorylation		Uniprot
S315	Phosphorylation		Uniprot
K325	Ubiquitination		Uniprot
K327	Ubiquitination		Uniprot

Research Backgrounds

P17535_JUND_HUMAN

Function:

Transcription factor binding AP-1 sites.

PTMs:

Phosphorylated by MAP kinases MAPK8 and MAPK10; phosphorylation is inhibited in the presence of MEN1.

Subcellular Location:

Nucleus.

Subunit Structure:

Binds DNA as a dimer (By similarity). Interacts (via MBM motif) with MEN1; this interaction represses transcriptional activation. Interacts with MAPK10; this interaction is inhibited in the presence of MEN1.

Family&Domains:

Belongs to the bZIP family. Jun subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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JunD Antibody - #AF7744