ATP1A1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat brain tissue sample,using ATP1 alpha1/Na+K+ ATPase1 Antibody(AF7700).

Product:	ATP1A1 Antibody
Catalog:	AF7700
Description:	Rabbit polyclonal antibody to ATP1A1
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	113kDa; 113kD(Calculated).
Uniprot:	P05023
RRID:	AB_2844064

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

ATP1A1 Antibody detects endogenous levels of total ATP1A1.

RRID:

AB_2844064
Cite Format: Affinity Biosciences Cat# AF7700, RRID:AB_2844064.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATPase Na+/K+ transporting alpha; adenosinetriphosphatase; AT1A1_HUMAN; ATP1A1; ATP1A4; ATP1AL2; ATP1B; ATP1B1; ATPase Na+/K+ transporting alpha 1 polypeptide; ATPase Na+/K+ transporting alpha 4 polypeptide; ATPase Na+/K+ transporting beta 1 polypeptide; ATPase, Na+/K+ transporting, alpha polypeptide-like 2; ATPase, Na+/K+ transporting, beta 1 polypeptide; Beta 1-subunit of Na(+),K(+)-ATPase; Na(+)/K(+) ATPase alpha-1 subunit; Na(+)/K(+) ATPase alpha-4 subunit; Na+, K+ ATPase alpha subunit; Na+/K+ ATPase 1; Na+/K+ ATPase 4; Na+/K+ ATPase, alpha-D polypeptide; Na, K-ATPase beta-1 polypeptide; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase catalytic subunit alpha-A protein; Na,K-ATPase subunit alpha-C; polypeptide-like 2; Sodium pump 1; sodium pump 4; Sodium pump subunit alpha-1; sodium pump subunit alpha-4; sodium pump subunit beta-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-4; sodium-potassium ATPase subunit beta 1 (non-catalytic); sodium-potassium ATPase, alpha 4 polypeptide; sodium-potassium-ATPase, alpha 1 polypeptide; Sodium/potassium transporting ATPase alpha 1 chain; Sodium/potassium transporting ATPase subunit beta 1; sodium/potassium-dependent ATPase beta-1 subunit; sodium/potassium-transporting ATPase alpha-4 chain; sodium/potassium-transporting ATPase beta-1 chain; Sodium/potassium-transporting ATPase subunit alpha-1; sodium/potassium-transporting ATPase subunit alpha-4;

Immunogens

Immunogen:

A synthesized peptide derived from Rat ATP1A1(#P06685), corresponding to a region within the internal amino acids.

Uniprot:

P05023(AT1A1_HUMAN) >>Visit HPA database.

Gene(ID):

ATP1A1(476)

Sequence:

P05023 AT1A1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGKGVGRDKYEPAAVSEQGDKKGKKGKKDRDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTSARAAEILARDGPNALTPPPTTPEWIKFCRQLFGGFSMLLWIGAILCFLAYSIQAATEEEPQNDNLYLGVVLSAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIRNGEKMSINAEEVVVGDLVEVKGGDRIPADLRIISANGCKVDNSSLTGESEPQTRSPDFTNENPLETRNIAFFSTNCVEGTARGIVVYTGDRTVMGRIATLASGLEGGQTPIAAEIEHFIHIITGVAVFLGVSFFILSLILEYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAESDIMKRQPRNPKTDKLVNERLISMAYGQIGMIQALGGFFTYFVILAENGFLPIHLLGLRVDWDDRWINDVEDSYGQQWTYEQRKIVEFTCHTAFFVSIVVVQWADLVICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMGVALRMYPLKPTWWFCAFPYSLLIFVYDEVRKLIIRRRPGGWVEKETYY

PTMs - P05023 As Substrate

P05023

Site	PTM Type	Enzyme	Source
K9	Ubiquitination		Uniprot
Y10	Phosphorylation		Uniprot
S16	Phosphorylation	Q05513 (PRKCZ) , P28482 (MAPK1)	Uniprot
K21	Ubiquitination		Uniprot
K36	Ubiquitination		Uniprot
K37	Ubiquitination		Uniprot
K45	Ubiquitination		Uniprot
S47	Phosphorylation		Uniprot
K54	Ubiquitination		Uniprot
Y55	Phosphorylation		Uniprot
S60	Phosphorylation		Uniprot
R61	Methylation		Uniprot
T81	Phosphorylation		Uniprot
T85	Phosphorylation		Uniprot
T86	Phosphorylation		Uniprot
Y149	Phosphorylation		Uniprot
K156	Ubiquitination		Uniprot
K162	Ubiquitination		Uniprot
K177	Ubiquitination		Uniprot
K194	Ubiquitination		Uniprot
R198	Methylation		Uniprot
R204	Methylation		Uniprot
S207	Phosphorylation		Uniprot
K212	Ubiquitination		Uniprot
S216	Phosphorylation		Uniprot
S217	Phosphorylation		Uniprot
S222	Phosphorylation		Uniprot
T226	Phosphorylation		Uniprot
S228	Phosphorylation		Uniprot
S246	Phosphorylation		Uniprot
C249	S-Nitrosylation		Uniprot
Y260	Phosphorylation		Uniprot
T261	Phosphorylation		Uniprot
R264	Methylation		Uniprot
T265	Phosphorylation		Uniprot
K359	Ubiquitination		Uniprot
S369	Phosphorylation		Uniprot
K377	Ubiquitination		Uniprot
T378	Phosphorylation		Uniprot
S407	Phosphorylation		Uniprot
K444	Ubiquitination		Uniprot
S452	Phosphorylation		Uniprot
K458	Ubiquitination		Uniprot
C459	S-Nitrosylation		Uniprot
C463	S-Nitrosylation		Uniprot
S466	Phosphorylation		Uniprot
K468	Ubiquitination		Uniprot
K476	Ubiquitination		Uniprot
S484	Phosphorylation		Uniprot
K487	Acetylation		Uniprot
K487	Ubiquitination		Uniprot
Y488	Phosphorylation		Uniprot
K494	Methylation		Uniprot
K494	Ubiquitination		Uniprot
T498	Phosphorylation		Uniprot
S499	Phosphorylation		Uniprot
K508	Ubiquitination		Uniprot
R517	Methylation		Uniprot
S520	Phosphorylation		Uniprot
K526	Sumoylation		Uniprot
K526	Ubiquitination		Uniprot
K535	Ubiquitination		Uniprot
Y542	Phosphorylation		Uniprot
K605	Ubiquitination		Uniprot
K612	Ubiquitination		Uniprot
T617	Phosphorylation		Uniprot
T623	Phosphorylation		Uniprot
K625	Ubiquitination		Uniprot
K629	Ubiquitination		Uniprot
T640	Phosphorylation		Uniprot
S653	Phosphorylation		Uniprot
K661	Ubiquitination		Uniprot
K671	Ubiquitination		Uniprot
K683	Ubiquitination		Uniprot
Y684	Phosphorylation		Uniprot
T686	Phosphorylation		Uniprot
K698	Ubiquitination		Uniprot
C705	S-Nitrosylation		Uniprot
S722	Phosphorylation		Uniprot
K726	Ubiquitination		Uniprot
K727	Ubiquitination		Uniprot
K773	Ubiquitination		Uniprot
K833	Ubiquitination		Uniprot
K840	Ubiquitination		Uniprot
K843	Ubiquitination		Uniprot
Y902	Phosphorylation		Uniprot
S943	Phosphorylation	P17612 (PRKACA)	Uniprot
K950	Ubiquitination		Uniprot
K952	Ubiquitination		Uniprot
K1019	Ubiquitination		Uniprot

Research Backgrounds

P05023_AT1A1_HUMAN

Function:

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

PTMs:

Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity).

Subcellular Location:

Cell membrane>Sarcolemma>Multi-pass membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Subunit Structure:

The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit. Interacts with regulatory subunit FXYD1 (By similarity). Interacts with regulatory subunit FXYD3. Interacts with SIK1 (By similarity). Binds the HLA class II histocompatibility antigen DR1. Interacts with SLC35G1 and STIM1.

Family&Domains:

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Organismal Systems > Circulatory system > Cardiac muscle contraction. (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

· Organismal Systems > Digestive system > Protein digestion and absorption.

· Organismal Systems > Digestive system > Mineral absorption.

References

1). CD36 as a molecular target of functional DNA aptamer NAFLD01 selected against NAFLD cells. ANALYTICAL CHEMISTRY, 2021 (PubMed: 33596054) [IF=7.4]

2). Adipose specific aptamer adipo-8 recognizes and interacts with APMAP to ameliorates fat deposition in vitro and in vivo. LIFE SCIENCES, 2020 (PubMed: 32272180) [IF=6.1]

Application: WB Species: mouse Sample: adipose

Fig. 1. |Adipo-8 recognized APMAP as its target specifically: (A) 3T3-L1 cell differentiation process and APMAP-silence by si-RNA. (B) Aptamer pull-down of 3T3-L1 adipocytes lysate: total membrane protein (lane A), membrane protein incubated with blank beads (lane B), adipo-8 beads (lane C), and library beads (lane D). The distinct band was marked by red arrow. (C) APMAP peptide fingerprint of mass spectrometry of distinct band. (D) Enrichment of APMAP in adipo-8 beads by immunoblotting.

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ATP1A1 Antibody - #AF7700