Cytokeratin 18 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat brain, using Keratin 18 Antibody.

Western blot analysis of extracts from MCF7 cells(H2O2 treatment), using Cytokeratin 18 Antibody.

Product:	Cytokeratin 18 Antibody
Catalog:	AF7692
Description:	Rabbit polyclonal antibody to Cytokeratin 18
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Rabbit
Mol.Wt.:	46kDa; 48kD(Calculated).
Uniprot:	P05783
RRID:	AB_2844056

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Rabbit(89%)

Clonality:

Polyclonal

Specificity:

Cytokeratin 18 Antibody detects endogenous levels of total Cytokeratin 18.

RRID:

AB_2844056
Cite Format: Affinity Biosciences Cat# AF7692, RRID:AB_2844056.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46; Cell proliferation-inducing gene 46 protein; CK 18; CK-18; CK18; CYK 18; CYK18; Cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; K 18; K18; K1C18_HUMAN; KA18; Keratin 18; Keratin 18, type I; Keratin D; keratin, type I cytoskeletal 18; Keratin-18; Krt18;

Immunogens

Immunogen:

Uniprot:

P05783(K1C18_HUMAN) >>Visit HPA database.

Gene(ID):

KRT18(3875)

Expression:

P05783 K1C18_HUMAN:

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

Sequence:

P05783 K1C18_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSRISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Rabbit

Zebrafish

Horse

Bovine

Sheep

Dog

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05783 As Substrate

P05783

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S2	Acetylation		Uniprot
S2	Phosphorylation		Uniprot
T4	Phosphorylation		Uniprot
S7	Phosphorylation		Uniprot
T8	Phosphorylation		Uniprot
S10	Phosphorylation		Uniprot
T11	Phosphorylation		Uniprot
Y13	Phosphorylation		Uniprot
S15	Phosphorylation		Uniprot
S18	Phosphorylation		Uniprot
S23	Phosphorylation		Uniprot
Y24	Phosphorylation		Uniprot
R27	Methylation		Uniprot
S30	O-Glycosylation		Uniprot
S30	Phosphorylation		Uniprot
S31	O-Glycosylation		Uniprot
S31	Phosphorylation		Uniprot
S34	Phosphorylation	P06493 (CDK1)	Uniprot
Y36	Phosphorylation		Uniprot
S42	Phosphorylation		Uniprot
S44	Phosphorylation		Uniprot
R45	Methylation		Uniprot
S47	Phosphorylation		Uniprot
S49	O-Glycosylation		Uniprot
S49	Phosphorylation		Uniprot
R50	Methylation		Uniprot
S51	Phosphorylation		Uniprot
T52	Phosphorylation		Uniprot
S53	Phosphorylation	P17252 (PRKCA) , Q9UBS0 (RPS6KB2) , P49137 (MAPKAPK2) , Q02156 (PRKCE) , P51812 (RPS6KA3) , Q14012 (CAMK1) , Q9UQM7 (CAMK2A)	Uniprot
R55	Methylation		Uniprot
S60	Phosphorylation		Uniprot
T65	Phosphorylation		Uniprot
K81	Acetylation		Uniprot
K81	Sumoylation		Uniprot
K81	Ubiquitination		Uniprot
T83	Phosphorylation		Uniprot
S86	Phosphorylation		Uniprot
R90	Methylation		Uniprot
S93	Phosphorylation		Uniprot
Y94	Phosphorylation		Uniprot
R97	Methylation		Uniprot
S100	Phosphorylation		Uniprot
S110	Phosphorylation		Uniprot
K111	Acetylation		Uniprot
K118	Methylation		Uniprot
K118	Ubiquitination		Uniprot
S127	Phosphorylation		Uniprot
Y129	Phosphorylation		Uniprot
K131	Acetylation		Uniprot
K131	Methylation		Uniprot
K131	Ubiquitination		Uniprot
K167	Acetylation		Uniprot
K167	Methylation		Uniprot
K167	Sumoylation		Uniprot
K167	Ubiquitination		Uniprot
Y168	Phosphorylation		Uniprot
S177	Phosphorylation		Uniprot
K187	Acetylation		Uniprot
K187	Methylation		Uniprot
K187	Sumoylation		Uniprot
K187	Ubiquitination		Uniprot
T192	Phosphorylation		Uniprot
T195	Phosphorylation		Uniprot
T201	Phosphorylation		Uniprot
K207	Sumoylation		Uniprot
K207	Ubiquitination		Uniprot
K214	Acetylation		Uniprot
K214	Ubiquitination		Uniprot
K215	Ubiquitination		Uniprot
K222	Acetylation		Uniprot
S230	Phosphorylation		Uniprot
K241	Ubiquitination		Uniprot
S242	Phosphorylation		Uniprot
K247	Acetylation		Uniprot
K247	Sumoylation		Uniprot
K247	Ubiquitination		Uniprot
Y256	Phosphorylation		Uniprot
K262	Ubiquitination		Uniprot
K269	Sumoylation		Uniprot
Y270	Phosphorylation		Uniprot
S272	Phosphorylation		Uniprot
T302	Phosphorylation		Uniprot
S305	Phosphorylation		Uniprot
S312	Phosphorylation		Uniprot
K317	Acetylation		Uniprot
K317	Sumoylation		Uniprot
K317	Ubiquitination		Uniprot
S319	Phosphorylation		Uniprot
S323	Phosphorylation		Uniprot
Y331	Phosphorylation		Uniprot
S347	Phosphorylation		Uniprot
Y363	Phosphorylation		Uniprot
K370	Ubiquitination		Uniprot
K372	Sumoylation		Uniprot
K372	Ubiquitination		Uniprot
T379	Phosphorylation		Uniprot
Y380	Phosphorylation		Uniprot
S398	Phosphorylation		Uniprot
S399	Phosphorylation		Uniprot
S401	Phosphorylation		Uniprot
T404	Phosphorylation		Uniprot
K407	Ubiquitination		Uniprot
T409	Phosphorylation		Uniprot
K417	Acetylation		Uniprot
K417	Sumoylation		Uniprot
K417	Ubiquitination		Uniprot
S420	Phosphorylation		Uniprot
T422	Phosphorylation		Uniprot
T425	Phosphorylation		Uniprot
K426	Acetylation		Uniprot
K426	Methylation		Uniprot
K426	Sumoylation		Uniprot
K426	Ubiquitination		Uniprot

Research Backgrounds

P05783_K1C18_HUMAN

Function:

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

PTMs:

Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.

Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.

O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.

Subcellular Location:

Cytoplasm>Perinuclear region. Nucleus>Nucleolus.

Tissue Specificity:

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. KRT18 associates with KRT8. Interacts with the thrombin-antithrombin complex (By similarity). Interacts with PNN and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD. Interacts with FAM83H. Interacts with EPPK1 (By similarity).

(Microbial infection) Interacts with hepatitis C virus/HCV core protein.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

Restrictive clause

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Cytokeratin 18 Antibody - #AF7692