NFAT5 Antibody - #AF7662
| Product: | NFAT5 Antibody |
| Catalog: | AF7662 |
| Description: | Rabbit polyclonal antibody to NFAT5 |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 160kDa; 166kD(Calculated). |
| Uniprot: | O94916 |
| RRID: | AB_2844026 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF7662, RRID:AB_2844026.
Fold/Unfold
Glutamine rich protein H65; KIAA0827; NF AT5; NF-AT5; NFAT 5; NFAT L1; NFAT like protein 1; NFAT5; NFAT5_HUMAN; NFATL 1; NFATL1; NFATZ; Nuclear factor of activated T cells 5; Nuclear factor of activated T cells 5 tonicity responsive; Nuclear factor of activated T cells; Nuclear factor of activated T-cells 5; OREBP; Osmotic response element binding protein; T cell transcription factor NFAT 5; T cell transcription factor NFAT5; T-cell transcription factor NFAT5; TonE binding protein; TonE-binding protein; TonEBP; Tonicity responsive enhancer binding protein; Tonicity-responsive enhancer-binding protein;
Immunogens
Widely expressed, with highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes.
- O94916 NFAT5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSDFISLLSADLDLESPKSLYSRESVYDLLPKELQLPPSRETSVASMSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O94916 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S17 | Phosphorylation | Uniprot | |
| Y28 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| C108 | S-Nitrosylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| K122 | Acetylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| T125 | Phosphorylation | Uniprot | |
| T128 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | P06493 (CDK1) , Q00535 (CDK5) | Uniprot |
| K137 | Ubiquitination | Uniprot | |
| T140 | Phosphorylation | Uniprot | |
| Y143 | Phosphorylation | P00519 (ABL1) | Uniprot |
| S145 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Q8N752 (CSNK1A1L) , P48729 (CSNK1A1) | Uniprot |
| S220 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| Y235 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| T241 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| K276 | Sumoylation | Uniprot | |
| K283 | Sumoylation | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| K556 | Sumoylation | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| K576 | Ubiquitination | Uniprot | |
| T594 | Phosphorylation | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S646 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| S684 | Phosphorylation | Uniprot | |
| S1197 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S1247 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S1367 | Phosphorylation | Q13315 (ATM) | Uniprot |
Research Backgrounds
Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3'.
Phosphorylated. Phosphorylated at Thr-135 by CDK5 in response to osmotic stress; this phosphorylation mediates its rapid nuclear localization.
Nucleus. Cytoplasm.
Note: Nuclear distribution increases under hypertonic conditions.
Widely expressed, with highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes.
Homodimer when bound to DNA, completely encircles its DNA target. Interacts with CIDEC; this interaction is direct and retains NFAT5 in the cytoplasm (By similarity). Does not bind with Fos and Jun transcription factors. Interacts with DDX5 and DDX17; this interaction leads to DDX5/DDX17 recruitment to LNC2 and S100A4 promoters and NFAT5-mediated DDX5/DDX17-enhanced transactivation.
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