STAM2 Antibody - #AF7658

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Product: STAM2 Antibody
Catalog: AF7658
Description: Rabbit polyclonal antibody to STAM2
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 58kDa; 58kD(Calculated).
Uniprot: O75886
RRID: AB_2844022

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(90%), Bovine(90%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(80%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
STAM2 Antibody detects endogenous levels of total STAM2.
RRID:
AB_2844022
Cite Format: Affinity Biosciences Cat# AF7658, RRID:AB_2844022.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp564C047; Hbp; Hrs-binding protein; HSE1 homolog; Signal transducing adapter molecule 2; Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2; Signal transducing adaptor molecule 2A; Signal transducing adaptor molecule 2B; Signal transducing adaptor molecule; STAM like protein containing SH3 and ITAM domains 2; STAM-2; Stam2; STAM2_HUMAN; STAM2A; STAM2B;

Immunogens

Immunogen:
Uniprot:

O75886(STAM2_HUMAN) >>Visit HPA database.

Gene(ID):
STAM2(10254)
Expression:
O75886 STAM2_HUMAN:

Ubiquitously expressed.

Sequence:
MPLFTANPFEQDVEKATNEYNTTEDWSLIMDICDKVGSTPNGAKDCLKAIMKRVNHKVPHVALQALTLLGACVANCGKIFHLEVCSRDFATEVRAVIKNKAHPKVCEKLKSLMVEWSEEFQKDPQFSLISATIKSMKEEGITFPPAGSQTVSAAAKNGTSSNKNKEDEDIAKAIELSLQEQKQQHTETKSLYPSSEIQLNNKVARKVRALYDFEAVEDNELTFKHGEIIIVLDDSDANWWKGENHRGIGLFPSNFVTTNLNIETEAAAVDKLNVIDDDVEEIKKSEPEPVYIDEDKMDRALQVLQSIDPTDSKPDSQDLLDLEDICQQMGPMIDEKLEEIDRKHSELSELNVKVLEALELYNKLVNEAPVYSVYSKLHPPAHYPPASSGVPMQTYPVQSHGGNYMGQSIHQVTVAQSYSLGPDQIGPLRSLPPNVNSSVTAQPAQTSYLSTGQDTVSNPTYMNQNSNLQSATGTTAYTQQMGMSVDMSSYQNTTSNLPQLAGFPVTVPAHPVAQQHTNYHQQPLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Pig
90
Bovine
90
Xenopus
90
Rabbit
90
Dog
80
Chicken
70
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75886 As Substrate

Site PTM Type Enzyme
Ubiquitination
K57 Ubiquitination
K104 Ubiquitination
K122 Ubiquitination
S127 Phosphorylation
K134 Ubiquitination
K156 Ubiquitination
T159 Phosphorylation
S160 Phosphorylation
S161 Phosphorylation
K165 Ubiquitination
K172 Ubiquitination
S177 Phosphorylation
K182 Ubiquitination
K189 Ubiquitination
S190 Phosphorylation
Y192 Phosphorylation
K202 Ubiquitination
K283 Ubiquitination
K284 Ubiquitination
Y291 Phosphorylation P06241 (FYN)
K296 Ubiquitination
K343 Ubiquitination
S345 Phosphorylation
K363 Ubiquitination
Y371 Phosphorylation P06241 (FYN)
S372 Phosphorylation
Y374 Phosphorylation P06241 (FYN)
S375 Phosphorylation
Y418 Phosphorylation

Research Backgrounds

Function:

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).

PTMs:

Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF.

Subcellular Location:

Cytoplasm. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 and EPS15. Interacts with JAK2 and JAK3. Interacts with ubiquitinated proteins and the deubiquitinating enzyme USP8/UBPY (By similarity). Interacts (via the via the PxVxL motif) with CBX5; the interaction is direct. Interacts with VPS37C. Interacts with ubiquitin; the interaction is direct. Interacts (via UIM domain) with UBQLN1 (via ubiquitin-like domain) (By similarity).

Family&Domains:

The VHS and UIM domains mediate the interaction with ubiquitinated proteins.

The SH3 domain mediates the interaction with USP8.

Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.

Belongs to the STAM family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

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