Osteopontin Antibody - #AF0227

Figure 4. Biocompatibility and osteogenic differentiation ability of HN25. G) Immunofluorescence staining of ALP and OPN after culturing 14 d on different samples. Scale bar = 100 μm. H) Volcano plot showing differently expressed genes in MSCs from HN25 under US irradiation compared to the control.

Figure 5. In vitro biocompatibilities and osteogenic activities of the substrates. (A) CCK-8 assay of hBMSCs on the indicated surfaces after 1, 3 and 7 days of culture. * denotes p < 0.05 and ** denotes p < 0.01 compared to Ti-NTs, # denotes p < 0.05 compared to the corresponding control group without peptides. Error bars denote the standard deviations over quadruplicate measurements with separately implants. (B) Confocal fluorescence microscopy images of hBMSCs stained with vinculin, F-actin and DAPI after being cultured for 24 h. Scale bar, 50 μm. (C-F) qRT-PCR assay of osteogenic gene expression of (C) ALP, (D) RUNX-2, (E) COL1 and (F) OPN of hBMSCs after 7 and 14 days of culture. * denotes p < 0.05, ** denotes p < 0.01 and *** denotes p < 0.001 compared to Ti-NTs-P-A; # denotes p < 0.05, & denotes p < 0.01 and $ denotes p < 0.001 compared to the corresponding control group without peptides. All error bars denote the standard deviations over quadruplicate measurements with separately implants. (G) Immunofluorescence staining of hBMSCs cultured on Ti-NTs-P-A for 7 days (ALP and RUNX-2) and 14 days (OPN). The images were obtained by confocal fluorescence microscopy. Scale bar, 50 μm. (H) Western blotting of hBMSCs cultured on the substrates for 7 and 14 days. At each time point, left lane was Ti-NTs, middle lane was Ti-NTs-A and right lane was Ti-NTs-P-A. * denotes p < 0.05, ** denotes p < 0.01 and *** denotes p < 0.001 compared to Ti-NTs-P-A. Error bars denote the standard deviations over triplicate measurements with separately Western blotting results.

Fig. 5 In vitro evaluation of C-T@Ti3C2 nanosheets for promoting osteogenesis. a Viability of rat bone marrow mesenchymal stem cells after incubation with different concentrations of C-T@Ti3C2 nanosheets (n = 3 for each group). b CLSM of AM/PI-stained rBMMSCs after various concentrations of C-T@Ti3C2 nanosheets. c CLSM images of the expression of BMP2, OCN, RUNX2 and OPN (at Day 14) in rBMMSCs after different treatments. A representative image of three replicates from each group is shown. d Relative mRNA levels of osteogenic genes (COL-I, BMP2, OPN and RUNX2). e Representative Western blots of total COL-I, OPN, BMP2, RUNX2 and β-actin after induction for 14 days in rBMMSCs. Data are presented as the mean ± SD, and statistical significance was calculated using the two-tailed t test

Fig. 5 In vitro evaluation of C-T@Ti3C2 nanosheets for promoting osteogenesis. a Viability of rat bone marrow mesenchymal stem cells after incubation with different concentrations of C-T@Ti3C2 nanosheets (n = 3 for each group). b CLSM of AM/PI-stained rBMMSCs after various concentrations of C-T@Ti3C2 nanosheets. c CLSM images of the expression of BMP2, OCN, RUNX2 and OPN (at Day 14) in rBMMSCs after different treatments. A representative image of three replicates from each group is shown. d Relative mRNA levels of osteogenic genes (COL-I, BMP2, OPN and RUNX2). e Representative Western blots of total COL-I, OPN, BMP2, RUNX2 and β-actin after induction for 14 days in rBMMSCs. Data are presented as the mean ± SD, and statistical significance was calculated using the two-tailed t test

Fig. 1. Effect of kaempferol on renal CaOx crystal deposition and tubular injury in vivo. Eligible mice were divided into five groups (n = 8). After a period of 10-day drug treatments, all mice were sacrificed on the 11 th day for the following tests. Pathological results were shown by the (a) images as well as (b-g) corresponding quantitative analysis through HE staining, Pizzolato staining, PAS staining, TUNEL staining, OPN and CD44 immunohistochemistry staining of paraffin embedded kidney sections. (h-j) Renal dysfunction was determined by serum levels of BUN, creatinine and NAGL among the five groups. (k) Group annotations were applied for all histograms. Data were expressed as the fold changes of experimental group to NC group or GA group, and were represented as means ± SD. * p < 0.05 vs. NC group; ** p < 0.05 as GA+Kae (25mg/kg) vs. GA group, GA + Kae (50 mg/kg) vs. GA group, and GA + Kae (25 mg/kg) vs. GA + Kae (50 mg/kg) group, respectively.