SF3B1 Antibody - #AF7653

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Product: SF3B1 Antibody
Catalog: AF7653
Description: Rabbit polyclonal antibody to SF3B1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 145kDa; 146kD(Calculated).
Uniprot: O75533
RRID: AB_2844017

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
SF3B1 Antibody detects endogenous levels of total SF3B1.
RRID:
AB_2844017
Cite Format: Affinity Biosciences Cat# AF7653, RRID:AB_2844017.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hsh 155; MDS; Pre mRNA processing 10; Pre mRNA splicing factor SF3b 155 kDa subunit; PRP 10; PRP10; PRPF 10; PRPF10; SAP 155; SAP155; SF3B 1; SF3b155; Spliceosome associated protein 155; Splicing factor 3b subunit 1 155kDa; Splicing factor 3B subunit 1;

Immunogens

Immunogen:
Uniprot:

O75533(SF3B1_HUMAN) >>Visit HPA database.

Gene(ID):
SF3B1(23451)
Sequence:
MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGGKTPDPKMNARTYMDVMREQHLTKEEREIRQQLAEKAKAGELKVVNGAAASQPPSKRKRRWDQTADQTPGATPKKLSSWDQAETPGHTPSLRWDETPGRAKGSETPGATPGSKIWDPTPSHTPAGAATPGRGDTPGHATPGHGGATSSARKNRWDETPKTERDTPGHGSGWAETPRTDRGGDSIGETPTPGASKRKSRWDETPASQMGGSTPVLTPGKTPIGTPAMNMATPTPGHIMSMTPEQLQAWRWEREIDERNRPLSDEELDAMFPEGYKVLPPPAGYVPIRTPARKLTATPTPLGGMTGFHMQTEDRTMKSVNDQPSGNLPFLKPDDIQYFDKLLVDVDESTLSPEEQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLLVKVIDRILYKLDDLVRPYVHKILVVIEPLLIDEDYYARVEGREIISNLAKAAGLATMISTMRPDIDNMDEYVRNTTARAFAVVASALGIPSLLPFLKAVCKSKKSWQARHTGIKIVQQIAILMGCAILPHLRSLVEIIEHGLVDEQQKVRTISALAIAALAEAATPYGIESFDSVLKPLWKGIRQHRGKGLAAFLKAIGYLIPLMDAEYANYYTREVMLILIREFQSPDEEMKKIVLKVVKQCCGTDGVEANYIKTEILPPFFKHFWQHRMALDRRNYRQLVDTTVELANKVGAAEIISRIVDDLKDEAEQYRKMVMETIEKIMGNLGAADIDHKLEEQLIDGILYAFQEQTTEDSVMLNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAKVRQQAADLISRTAVVMKTCQEEKLMGHLGVVLYEYLGEEYPEVLGSILGALKAIVNVIGMHKMTPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGAEYVSAREWMRICFELLELLKAHKKAIRRATVNTFGYIAKAIGPHDVLATLLNNLKVQERQNRVCTTVAIAIVAETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLLEDALMDRDLVHRQTASAVVQHMSLGVYGFGCEDSLNHLLNYVWPNVFETSPHVIQAVMGALEGLRVAIGPCRMLQYCLQGLFHPARKVRDVYWKIYNSIYIGSQDALIAHYPRIYNDDKNTYIRYELDYIL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75533 As Substrate

Site PTM Type Enzyme
K6 Ubiquitination
K21 Ubiquitination
K22 Ubiquitination
T36 Phosphorylation
Y38 Phosphorylation
Y39 Phosphorylation
Y44 Phosphorylation
T61 Phosphorylation
Y70 Phosphorylation
S72 Phosphorylation
S73 Phosphorylation
S75 Phosphorylation
K81 Ubiquitination
Y84 Phosphorylation
Y101 Phosphorylation
Y119 Phosphorylation
T125 Phosphorylation
S129 Phosphorylation
K141 Acetylation
K141 Ubiquitination
T142 Phosphorylation P24941 (CDK2)
K146 Ubiquitination
Y152 Phosphorylation
K175 Ubiquitination
K182 Ubiquitination
S190 Phosphorylation
S194 Phosphorylation
K195 Acetylation
K195 Methylation
K195 Ubiquitination
T203 Phosphorylation
T207 Phosphorylation
T211 Phosphorylation P24941 (CDK2)
K213 Ubiquitination
K214 Sumoylation
K214 Ubiquitination
S216 Phosphorylation
S217 Phosphorylation
T223 Phosphorylation
T227 Phosphorylation
S229 Phosphorylation
R231 Methylation
T235 Phosphorylation
K240 Ubiquitination
S242 Phosphorylation
T244 Phosphorylation P24941 (CDK2)
T248 Phosphorylation P24941 (CDK2)
S251 Phosphorylation
T257 Phosphorylation
S259 Phosphorylation P24941 (CDK2)
T261 Phosphorylation P24941 (CDK2)
T267 Phosphorylation P24941 (CDK2)
T273 Phosphorylation Q13627 (DYRK1A)
T278 Phosphorylation
T285 Phosphorylation
S286 Phosphorylation
S287 Phosphorylation
T296 Phosphorylation
T299 Phosphorylation
T303 Phosphorylation
S308 Phosphorylation
T313 Phosphorylation P24941 (CDK2)
T316 Phosphorylation
R318 Methylation
S322 Phosphorylation
T326 Phosphorylation
T328 Phosphorylation
S332 Phosphorylation
K333 Acetylation
K333 Ubiquitination
T341 Phosphorylation
S344 Phosphorylation
S349 Phosphorylation
T350 Phosphorylation
T354 Phosphorylation P24941 (CDK2)
T362 Phosphorylation
T369 Phosphorylation
T371 Phosphorylation
S377 Phosphorylation
T379 Phosphorylation
S400 Phosphorylation
K413 Sumoylation
K413 Ubiquitination
Y421 Phosphorylation
T426 Phosphorylation P24941 (CDK2)
T432 Phosphorylation P24941 (CDK2)
T434 Phosphorylation Q9Y463 (DYRK1B) , P24941 (CDK2) , Q13627 (DYRK1A)
T436 Phosphorylation P24941 (CDK2)
T442 Phosphorylation
K454 Ubiquitination
K468 Sumoylation
K468 Ubiquitination
Y474 Phosphorylation
K477 Ubiquitination
S488 Phosphorylation
K493 Ubiquitination
K499 Sumoylation
K499 Ubiquitination
T508 Phosphorylation
R517 Methylation
S541 Phosphorylation
K554 Acetylation
K554 Ubiquitination
R558 Methylation
K562 Acetylation
K700 Ubiquitination
K733 Ubiquitination
Y761 Phosphorylation
Y764 Phosphorylation
K785 Ubiquitination
K790 Ubiquitination
K793 Ubiquitination
T808 Phosphorylation
K816 Ubiquitination
S851 Phosphorylation
K858 Ubiquitination
K866 Ubiquitination
K874 Ubiquitination
K926 Ubiquitination
K963 Ubiquitination
K1008 Ubiquitination
K1014 Ubiquitination
T1021 Phosphorylation
K1025 Ubiquitination
K1030 Ubiquitination
R1045 Methylation
C1059 S-Nitrosylation
K1067 Acetylation
K1102 Ubiquitination
Y1136 Phosphorylation
T1170 Phosphorylation
R1180 Methylation
Y1288 Phosphorylation
K1292 Ubiquitination
Y1295 Phosphorylation
Y1302 Phosphorylation

Research Backgrounds

Function:

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity). May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

PTMs:

Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.

Citrullinated by PADI4.

Subcellular Location:

Nucleus. Nucleus speckle.
Note: During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the B-WICH complex, at least composed of SMARCA5/SNF2H, BAZ1B/WSTF, SF3B1, DEK, MYO1C, ERCC6, MYBBP1A and DDX21. Identified in the spliceosome C complex. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts directly with the splicing factor U2AF. Within the SF3B complex interacts directly (via HEAT domain) with SF3B3, SF3B5 and (via HEAT domain) with PHF5A. Interacts directly with SF3B6 Ref.36,. The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Phosphorylated form interacts with PPP1R8. Interacts with PQBP1. Interacts with RBM17. Interacts with RBM39. Interacts with SETX. Interacts with RBM15.

Family&Domains:

Belongs to the SF3B1 family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

References

1). Mangiferin Inhibits PDGF-BB-Induced Proliferation and Migration of Rat Vascular Smooth Muscle Cells and Alleviates Neointimal Formation in Mice through the AMPK/Drp1 Axis. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34900084)

Application: WB    Species: Mouse    Sample:

Figure 4 Mangiferin alleviated PDGF-BB-induced mitochondrial fission and increase in ROS levels. (a) RSMCs were stained with mitotracker probe after treatment with different concentrations of mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. The average length of mitochondria was quantified using ImageJ software. (b) Intracellular ROS levels were detected using flow cytometry after RSMCs were treated with mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. (c–e) Western blot was used to measure the protein expression levels of AMPK, pAMPKThr172, Drp1, and pDrp1Ser616 after RSMCs were treated with various concentrations of mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. All assays were conducted in triplicate and all data presented as mean ± S.D. ∗P < 0.05, compared with the control group. #P < 0.05, compared with the PDGF-BB group.

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