Phospho-CDC25B (Ser353) Antibody - #AF3257
| Product: | Phospho-CDC25B (Ser353) Antibody |
| Catalog: | AF3257 |
| Description: | Rabbit polyclonal antibody to Phospho-CDC25B (Ser353) |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Monkey |
| Prediction: | Bovine, Horse, Sheep |
| Mol.Wt.: | 64kDa; 65kD(Calculated). |
| Uniprot: | P30305 |
| RRID: | AB_2834683 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3257, RRID:AB_2834683.
Fold/Unfold
AI604853; Cdc 25B; Cdc25b; CDC25HU2; Cdc25m2; Cell division cycle 25 homolog B; Cell division cycle 25B; Cell division cycle 25B isoform 1; Cell division cycle 25B isoform 2; Cell division cycle 25B isoform 3; Cell division cycle 25B isoform 4; Cell division cycle 25B isoform 5; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M phase inducer phosphatase 2; M-phase inducer phosphatase 2; MPIP2_HUMAN;
Immunogens
- P30305 MPIP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEVPQPEPAPGSALSPAGVCGGAQRPGHLPGLLLGSHGLLGSPVRAAASSPVTTLTQTMHDLAGLGSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P30305 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S15 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | P53350 (PLK1) | Uniprot |
| T53 | Phosphorylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| T56 | Phosphorylation | Uniprot | |
| T58 | Phosphorylation | P53350 (PLK1) | Uniprot |
| T69 | Phosphorylation | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| T86 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| S117 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| T127 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S151 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S160 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T167 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S169 | Phosphorylation | Q14680 (MELK) , P49137 (MAPKAPK2) | Uniprot |
| K177 | Ubiquitination | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S187 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S188 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| S209 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S219 | Phosphorylation | Q14680 (MELK) | Uniprot |
| S229 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | P17612 (PRKACA) , O14757 (CHEK1) | Uniprot |
| S238 | Phosphorylation | Uniprot | |
| K242 | Sumoylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| S249 | Phosphorylation | Q15759 (MAPK11) | Uniprot |
| T265 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S291 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K299 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S323 | Phosphorylation | Q16539 (MAPK14) , P17612 (PRKACA) , P49137 (MAPKAPK2) , Q14680 (MELK) | Uniprot |
| S327 | Phosphorylation | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| T344 | Phosphorylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| S353 | Phosphorylation | P53350 (PLK1) , P49137 (MAPKAPK2) , Q15418 (RPS6KA1) , O14965 (AURKA) , P31749 (AKT1) | Uniprot |
| T355 | Phosphorylation | Q15418 (RPS6KA1) | Uniprot |
| K367 | Sumoylation | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| S375 | Phosphorylation | P49137 (MAPKAPK2) , Q8TDC3-2 (BRSK1) , Q16539 (MAPK14) , P53350 (PLK1) | Uniprot |
| S387 | Phosphorylation | Uniprot | |
| S397 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K398 | Ubiquitination | Uniprot | |
| T404 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K408 | Ubiquitination | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| S465 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K469 | Ubiquitination | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| K479 | Ubiquitination | Uniprot | |
| S513 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K523 | Ubiquitination | Uniprot | |
| K527 | Ubiquitination | Uniprot | |
| K551 | Ubiquitination | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| T556 | Phosphorylation | Uniprot | |
| S563 | Phosphorylation | O14757 (CHEK1) | Uniprot |
Research Backgrounds
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole.
Interacts with MAPK14 and 14-3-3 proteins.
Belongs to the MPI phosphatase family.
Research Fields
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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