CTNND1 Antibody | Affinity Biosciences

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Western blot analysis of CTNND1 in lysates of HuvEc, using CTNND1 Antibody(AF7636).

Product:	CTNND1 Antibody
Catalog:	AF7636
Description:	Rabbit polyclonal antibody to CTNND1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Rabbit
Mol.Wt.:	105kDa; 108kD(Calculated).
Uniprot:	O60716
RRID:	AB_2844000

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

CTNND1 Antibody detects endogenous levels of total CTNND1.

RRID:

AB_2844000
Cite Format: Affinity Biosciences Cat# AF7636, RRID:AB_2844000.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cadherin associated Src substrate; Cadherin-associated Src substrate; CAS; Catenin (cadherin associated protein) delta 1; Catenin delta 1; Catenin delta; Catenin delta-1; CTND1_HUMAN; CTNND 1; CTNND; CTNND1; KIAA0384; p120; P120 CAS; p120 catenin; P120 CTN; p120(cas); p120(ctn); P120CAS; P120CTN;

Immunogens

Immunogen:

Uniprot:

O60716(CTND1_HUMAN) >>Visit HPA database.

Gene(ID):

CTNND1(1500)

Expression:

O60716 CTND1_HUMAN:

Expressed in vascular endothelium. Melanocytes and melanoma cells primarily express the long isoform 1A, whereas keratinocytes express shorter isoforms, especially 3A. The shortest isoform 4A, is detected in normal keratinocytes and melanocytes, and generally lost from cells derived from squamous cell carcinomas or melanomas. The C-terminal alternatively spliced exon B is present in the p120ctn transcripts in the colon, intestine and prostate, but lost in several tumor tissues derived from these organs.

Sequence:

O60716 CTND1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDDSEVESTASILASVKEQEAQFEKLTRALEEERRHVSAQLERVRVSPQDANPLMANGTLTRRHQNGRFVGDADLERQKFSDLKLNGPQDHSHLLYSTIPRMQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTRRTETTVKKVVKTVTTRTVQPVAMGPDGLPVDASSVSNNYIQTLGRDFRKNGNGGPGPYVGQAGTATLPRNFHYPPDGYSRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQPQVRVGGSSVDLHRFHPEPYGLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPSDQYYWAPLAQHERGSLASLDSLRKGGPPPPNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPVLVGLLDHPKKEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIVDHALHALTDEVIIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIFIVQAEIGQKDSDSKLVENCVCLLRNLSYQVHREIPQAERYQEAAPNVANNTGPHAASCFGAKKGKDEWFSRGKKPIEDPANDTVDFPKRTSPARGYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTPNERGDHNRTLDRSGDLGDMEPLKGTTPLMQDEGQESLEEELDVLVLDDEGGQVSYPSMQKI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Zebrafish

100

Rabbit

100

Xenopus

Sheep

Dog

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60716 As Substrate

O60716

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S4	Phosphorylation		Uniprot
K25	Ubiquitination		Uniprot
T27	Phosphorylation		Uniprot
S38	Phosphorylation		Uniprot
S47	Phosphorylation		Uniprot
T59	Phosphorylation		Uniprot
T61	Phosphorylation		Uniprot
K79	Ubiquitination		Uniprot
S81	Phosphorylation		Uniprot
K84	Ubiquitination		Uniprot
Y96	Phosphorylation		Uniprot
S97	Phosphorylation		Uniprot
T98	Phosphorylation		Uniprot
R101	Methylation		Uniprot
Y112	Phosphorylation	P06241 (FYN)	Uniprot
S125	Phosphorylation		Uniprot
S129	Phosphorylation		Uniprot
T133	Phosphorylation		Uniprot
T134	Phosphorylation		Uniprot
K146	Ubiquitination		Uniprot
T147	Phosphorylation		Uniprot
T149	Phosphorylation		Uniprot
T152	Phosphorylation		Uniprot
S168	Phosphorylation		Uniprot
S169	Phosphorylation		Uniprot
S171	Phosphorylation		Uniprot
Y174	Phosphorylation		Uniprot
T177	Phosphorylation		Uniprot
K184	Ubiquitination		Uniprot
Y193	Phosphorylation		Uniprot
T199	Phosphorylation		Uniprot
T201	Phosphorylation		Uniprot
Y208	Phosphorylation		Uniprot
Y213	Phosphorylation		Uniprot
S214	Phosphorylation		Uniprot
Y217	Phosphorylation	P12931 (SRC)	Uniprot
Y221	Phosphorylation		Uniprot
S225	Phosphorylation		Uniprot
Y228	Phosphorylation	P00533 (EGFR) , P12931 (SRC)	Uniprot
S230	Phosphorylation		Uniprot
S232	Phosphorylation		Uniprot
T235	Phosphorylation		Uniprot
Y241	Phosphorylation		Uniprot
R242	Methylation		Uniprot
S244	Phosphorylation		Uniprot
Y248	Phosphorylation		Uniprot
R249	Methylation		Uniprot
S252	Phosphorylation		Uniprot
Y257	Phosphorylation		Uniprot
S268	Phosphorylation	Q02156 (PRKCE) , P49674 (CSNK1E)	Uniprot
S269	Phosphorylation		Uniprot
Y280	Phosphorylation		Uniprot
S288	Phosphorylation		Uniprot
Y291	Phosphorylation		Uniprot
Y296	Phosphorylation		Uniprot
S300	Phosphorylation		Uniprot
Y302	Phosphorylation		Uniprot
T304	Phosphorylation		Uniprot
T308	Phosphorylation		Uniprot
T310	Phosphorylation		Uniprot
S312	Phosphorylation		Uniprot
S320	Phosphorylation		Uniprot
Y321	Phosphorylation		Uniprot
S331	Phosphorylation		Uniprot
Y334	Phosphorylation		Uniprot
Y335	Phosphorylation		Uniprot
S346	Phosphorylation		Uniprot
S349	Phosphorylation		Uniprot
S352	Phosphorylation		Uniprot
K355	Acetylation		Uniprot
K355	Ubiquitination		Uniprot
C394	S-Nitrosylation		Uniprot
Y395	Phosphorylation		Uniprot
K408	Ubiquitination		Uniprot
K421	Ubiquitination		Uniprot
K422	Ubiquitination		Uniprot
K433	Ubiquitination		Uniprot
K444	Ubiquitination		Uniprot
T469	Phosphorylation		Uniprot
K517	Ubiquitination		Uniprot
S539	Phosphorylation		Uniprot
S542	Phosphorylation		Uniprot
K574	Ubiquitination		Uniprot
S587	Phosphorylation		Uniprot
Y588	Phosphorylation		Uniprot
Y600	Phosphorylation		Uniprot
T611	Phosphorylation		Uniprot
S617	Phosphorylation		Uniprot
C618	S-Nitrosylation		Uniprot
K622	Acetylation		Uniprot
K622	Ubiquitination		Uniprot
K623	Acetylation		Uniprot
K633	Ubiquitination		Uniprot
K634	Ubiquitination		Uniprot
T643	Phosphorylation		Uniprot
T650	Phosphorylation		Uniprot
S651	Phosphorylation		Uniprot
Y656	Phosphorylation		Uniprot
Y668	Phosphorylation		Uniprot
S670	Phosphorylation		Uniprot
S675	Phosphorylation		Uniprot
K676	Ubiquitination		Uniprot
K710	Ubiquitination		Uniprot
S713	Phosphorylation		Uniprot
T720	Phosphorylation		Uniprot
K728	Ubiquitination		Uniprot
S731	Phosphorylation		Uniprot
R735	Methylation		Uniprot
K744	Ubiquitination		Uniprot
K749	Ubiquitination		Uniprot
T774	Phosphorylation		Uniprot
S777	Phosphorylation		Uniprot
K804	Ubiquitination		Uniprot
K810	Ubiquitination		Uniprot
S811	Phosphorylation		Uniprot
K841	Ubiquitination		Uniprot
K846	Ubiquitination		Uniprot
S847	Phosphorylation		Uniprot
S857	Phosphorylation		Uniprot
S859	Phosphorylation		Uniprot
S861	Phosphorylation		Uniprot
S862	Phosphorylation		Uniprot
S864	Phosphorylation		Uniprot
Y865	Phosphorylation		Uniprot
S868	Phosphorylation		Uniprot
T869	Phosphorylation		Uniprot
K878	Ubiquitination		Uniprot
S879	Phosphorylation	P17252 (PRKCA) , P17612 (PRKACA)	Uniprot
K881	Ubiquitination		Uniprot
K882	Sumoylation		Uniprot
K882	Ubiquitination		Uniprot
S895	Phosphorylation		Uniprot
K898	Ubiquitination		Uniprot
S899	Phosphorylation		Uniprot
Y904	Phosphorylation		Uniprot
S905	Phosphorylation		Uniprot
T906	Phosphorylation		Uniprot
T916	Phosphorylation		Uniprot
S920	Phosphorylation		Uniprot
T932	Phosphorylation		Uniprot
T933	Phosphorylation		Uniprot
S943	Phosphorylation		Uniprot
Y962	Phosphorylation		Uniprot
S964	Phosphorylation		Uniprot

Research Backgrounds

O60716_CTND1_HUMAN

Function:

Key regulator of cell-cell adhesion that associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability. Beside cell-cell adhesion, regulates gene transcription through several transcription factors including ZBTB33/Kaiso2 and GLIS2, and the activity of Rho family GTPases and downstream cytoskeletal dynamics. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors.

PTMs:

Phosphorylated by FER and other protein-tyrosine kinases. Phosphorylated at Ser-288 by PAK5. Dephosphorylated by PTPRJ.

Subcellular Location:

Cell junction>Adherens junction. Cytoplasm. Nucleus. Cell membrane.
Note: Interaction with GLIS2 promotes nuclear translocation (By similarity). Detected at cell-cell contacts (PubMed:15240885, PubMed:17047063). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm (PubMed:17047063). CDH1 enhances cell membrane localization (PubMed:15240885). Isoforms 4A and 1AB are excluded from the nucleus (PubMed:11896187).

Nucleus.

Nucleus.

Nucleus.

Tissue Specificity:

Subunit Structure:

Belongs to a multiprotein cell-cell adhesion complex that also contains E-cadherin/CDH1, alpha-catenin/CTNNA1, beta-catenin/CTNNB1, and gamma-catenin/JUP. Component of a cadherin:catenin adhesion complex composed of at least of CDH26, beta-catenin/CTNNB1, alpha-catenin/CTNNA1 and p120 catenin/CTNND1. Binds to the C-terminal fragment of PSEN1 and mutually competes for CDH1. Interacts with ZBTB33. Interacts with GLIS2. Interacts with FER. Interacts with NANOS1 (via N-terminal region). Interacts (via N-terminus) with GNA12; the interaction regulates CDH1-mediated cell-cell adhesion. Interacts with GNA13. Interacts with CCDC85B. Interacts with PLPP3; negatively regulates the PLPP3-mediated stabilization of CTNNB1.

Family&Domains:

A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.

ARM repeats 1 to 5 mediate interaction with cadherins.

Belongs to the beta-catenin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

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CTNND1 Antibody - #AF7636