Product: MDM4 Antibody
Catalog: AF7609
Description: Rabbit polyclonal antibody to MDM4
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 55kDa,75kDa, 70 kDa; 55kD(Calculated).
Uniprot: O15151
RRID: AB_2843973

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(86%), Horse(100%), Sheep(86%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
MDM4 Antibody detects endogenous levels of total MDM4.
RRID:
AB_2843973
Cite Format: Affinity Biosciences Cat# AF7609, RRID:AB_2843973.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp781B1423; Double minute 4; Double minute 4 human homolog of p53 binding protein; Double minute 4 protein; HDMX; MDM 4; Mdm2 like p53 binding protein; Mdm2-like p53-binding protein; MDM4; Mdm4 p53 binding protein homolog mouse; Mdm4 protein; MDM4 related protein 1; Mdm4 transformed 3T3 cell double minute 4; Mdm4 transformed 3T3 cell double minute 4 p53 binding protein; Mdm4 transformed 3T3 cell double minute 4 p53 binding protein mouse; MDM4_HUMAN; Mdmx protein; MGC132766; Mouse double minute 4 homolog; Mouse double minute 4 human homolog of p53 binding protein; MRP 1; MRP1; p53 binding protein; p53 BINDING PROTEIN MDM4; p53-binding protein Mdm4; Protein Mdm4; Protein Mdmx;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O15151 MDM4_HUMAN:

Expressed in all tissues tested with high levels in thymus.

Sequence:
MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLKILHAAGAQGEMFTVKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRKNLVTLATATTDAAQTLALAQDHSMDIPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLAQDETSRLDLGFEEWDVAGLPWWFLGNLRSNYTPRSNGSTDLQTNQDVGTAIVSDTTDDLWFLNESVSEQLGVGIKVEAADTEQTSEEVGKVSDKKVIEVGKNDDLEDSKSLSDDTDVEVTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTHSLSTSDITAIPEKENEGNDVPDCRRTISAPVVRPKDAYIKKENSKLFDPCNSVEFLDLAHSSESQETISSMGEQLDNLSEQRTDTENMEDCQNLLKPCSLCEKRPRDGNIIHGRTGHLVTCFHCARRLKKAGASCPICKKEIQLVIKVFIA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Chicken
100
Rabbit
100
Bovine
86
Sheep
86
Xenopus
71
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15151 As Substrate

Site PTM Type Enzyme
Ubiquitination
S15 Phosphorylation
S20 Phosphorylation
Y55 Phosphorylation P00519 (ABL1)
K93 Ubiquitination
S96 Phosphorylation P06493 (CDK1)
Y99 Phosphorylation P00519 (ABL1)
T119 Phosphorylation
S161 Phosphorylation
K254 Sumoylation
T263 Phosphorylation
S264 Phosphorylation
S289 Phosphorylation P48729 (CSNK1A1)
S314 Phosphorylation
K336 Ubiquitination
S342 Phosphorylation P54646 (PRKAA2) , Q13315 (ATM) , O96017 (CHEK2) , O14757 (CHEK1)
T365 Phosphorylation
S367 Phosphorylation P31749 (AKT1) , Q13315 (ATM) , O14757 (CHEK1) , O96017 (CHEK2)
K374 Ubiquitination
K379 Sumoylation
K384 Ubiquitination
S403 Phosphorylation Q13315 (ATM)

Research Backgrounds

Function:

Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.

PTMs:

Phosphorylated. Phosphorylation at Ser-367 promotes interaction with YWHAG and subsequent ubiquitination and degradation. Phosphorylation at Ser-342 also induces ubiquitination and degradation but to a lower extent.

Ubiquitinated and degraded by MDM2. Deubiquitination by USP2 on the other hand stabilizes the MDM4 protein.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues tested with high levels in thymus.

Subunit Structure:

Interacts with MDM2, TP53, TP73 and USP2. Found in a trimeric complex with USP2, MDM2 and MDM4. Interacts (phosphorylated) with YWHAG; negatively regulates MDM4 activity toward TP53.

Family&Domains:

Region I is sufficient for binding TP53 and inhibiting its G1 arrest and apoptosis functions. It also binds TP73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.

Belongs to the MDM2/MDM4 family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

References

1). miR‐485's anti‐drug resistant epilepsy effects by regulating SV2A/PSD‐95 and targeting ABCC1 and neuronal signaling‐transduction proteins in hippocampus of rats. Brain and Behavior, 2021 (PubMed: 34291586) [IF=3.1]

Application: WB    Species: Rat    Sample: Hippocampus tissue

FIGURE 6 The expression of neuronal signaling‐transduction proteins in hippocampus of DRE rats at 9 weeks after SE. (a) Western blotting bands; (b) barographs of expression levels. *p < .05 versus the control group; # p < .05 versus the epilepsy group

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