Semaphorin 4B Antibody | Affinity Biosciences

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Western blot analysis of Semaphorin 4B in lysates of MIA-PACA2, using Semaphorin 4B Antibody(AF7598).

Western blot analysis of extracts from 293t, using Semaphorin 4B Antibody.

Western blot analysis of extracts from Mouse testis, using Semaphorin 4B Antibody at 1/1000 dilution.

Product:	Semaphorin 4B Antibody
Catalog:	AF7598
Description:	Rabbit polyclonal antibody to Semaphorin 4B
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	105KD; 93kD(Calculated).
Uniprot:	Q9NPR2
RRID:	AB_2843962

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$350	In stock
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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(90%), Horse(100%), Sheep(90%), Rabbit(90%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Semaphorin 4B Antibody detects endogenous levels of total Semaphorin 4B.

RRID:

AB_2843962
Cite Format: Affinity Biosciences Cat# AF7598, RRID:AB_2843962.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

KIAA1745; MGC131831; Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4B; Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain 4B; SEMA4B; SEMAC; Semaphorin 4B; SemC;

Immunogens

Immunogen:

Uniprot:

Q9NPR2(SEM4B_HUMAN) >>Visit HPA database.

Gene(ID):

SEMA4B(10509)

Sequence:

Q9NPR2 SEM4B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLRTAMGLRSWLAAPWGALPPRPPLLLLLLLLLLLQPPPPTWALSPRISLPLGSEERPFLRFEAEHISNYTALLLSRDGRTLYVGAREALFALSSNLSFLPGGEYQELLWGADAEKKQQCSFKGKDPQRDCQNYIKILLPLSGSHLFTCGTAAFSPMCTYINMENFTLARDEKGNVLLEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDPAISRSQSLRPTKTESSLNWLQDPAFVASAYIPESLGSLQGDDDKIYFFFSETGQEFEFFENTIVSRIARICKGDEGGERVLQQRWTSFLKAQLLCSRPDDGFPFNVLQDVFTLSPSPQDWRDTLFYGVFTSQWHRGTTEGSAVCVFTMKDVQRVFSGLYKEVNRETQQWYTVTHPVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQVRSRMLLLQPQARYQRVAVHRVPGLHHTYDVLFLGTGDGRLHKAVSVGPRVHIIEELQIFSSGQPVQNLLLDTHRGLLYAASHSGVVQVPMANCSLYRSCGDCLLARDPYCAWSGSSCKHVSLYQPQLATRPWIQDIEGASAKDLCSASSVVSPSFVPTGEKPCEQVQFQPNTVNTLACPLLSNLATRLWLRNGAPVNASASCHVLPTGDLLLVGTQQLGEFQCWSLEEGFQQLVASYCPEVVEDGVADQTDEGGSVPVIISTSRVSAPAGGKASWGADRSYWKEFLVMCTLFVLAVLLPVLFLLYRHRNSMKVFLKQGECASVHPKTCPVVLPPETRPLNGLGPPSTPLDHRGYQSLSDSPPGSRVFTESEKRPLSIQDSFVEVSPVCPRPRVRLGSEIRDSVV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Xenopus

100

Bovine

Sheep

Rabbit

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NPR2 As Substrate

Q9NPR2

Site	PTM Type	Source
N64	N-Glycosylation	Uniprot
Y65	Phosphorylation	Uniprot
T66	Phosphorylation	Uniprot
K403	Acetylation	Uniprot
N405	N-Glycosylation	Uniprot
S694	Phosphorylation	Uniprot
K740	Ubiquitination	Uniprot
K744	Ubiquitination	Uniprot
T764	Phosphorylation	Uniprot
S774	Phosphorylation	Uniprot
T775	Phosphorylation	Uniprot
Y782	Phosphorylation	Uniprot
S784	Phosphorylation	Uniprot
S786	Phosphorylation	Uniprot
S788	Phosphorylation	Uniprot
T796	Phosphorylation	Uniprot
K800	Ubiquitination	Uniprot
S804	Phosphorylation	Uniprot
S808	Phosphorylation	Uniprot
S813	Phosphorylation	Uniprot
S825	Phosphorylation	Uniprot
S830	Phosphorylation	Uniprot

Research Backgrounds

Q9NPR2_SEM4B_HUMAN

Function:

Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Family&Domains:

Belongs to the semaphorin family.

Research Fields

· Organismal Systems > Development > Axon guidance. (View pathway)

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Semaphorin 4B Antibody - #AF7598