Annexin A2 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from MCF7, using ANXA2 Antibody.

Product:	Annexin A2 Antibody
Catalog:	AF7597
Description:	Rabbit polyclonal antibody to Annexin A2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	37kDa; 39kD(Calculated).
Uniprot:	P07355
RRID:	AB_2843961

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(90%)

Clonality:

Polyclonal

Specificity:

Annexin A2 Antibody detects endogenous levels of total Annexin A2.

RRID:

AB_2843961
Cite Format: Affinity Biosciences Cat# AF7597, RRID:AB_2843961.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Annexin A2; Annexin II; Annexin II, heavy chain; Annexin-2; ANX 2; ANX2; ANX2L4; ANXA2; ANXA2_HUMAN; arylsulfatase B; CAL1H; Calpactin I heavy chain; calpactin I heavy polypeptide (p36); Calpactin I heavy polypeptide; Calpactin-1 heavy chain; chromobindin 8; Chromobindin-8; Epididymis secretory protein Li 270; HEL S 270; LIP2; Lipocortin II; LPC2; LPC2D; p36; P36 protein; PAP-IV; Placental anticoagulant protein IV; Protein I;

Immunogens

Immunogen:

Uniprot:

P07355(ANXA2_HUMAN) >>Visit HPA database.

Gene(ID):

ANXA2(302)

Sequence:

P07355 ANXA2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07355 As Substrate

P07355

Site	PTM Type	Enzyme	Source
S2	Phosphorylation		Uniprot
T3	Phosphorylation		Uniprot
K10	Acetylation		Uniprot
K10	Ubiquitination		Uniprot
S12	Phosphorylation	P17252 (PRKCA)	Uniprot
S18	Phosphorylation		Uniprot
T19	Phosphorylation		Uniprot
S22	Phosphorylation		Uniprot
Y24	Phosphorylation	P12931 (SRC)	Uniprot
S26	Phosphorylation	P05771 (PRKCB) , P17252 (PRKCA)	Uniprot
K28	Ubiquitination		Uniprot
Y30	Phosphorylation		Uniprot
T31	Phosphorylation		Uniprot
T44	Phosphorylation		Uniprot
K47	Acetylation		Uniprot
K47	Ubiquitination		Uniprot
K49	Acetylation		Uniprot
K49	Sumoylation		Uniprot
K49	Ubiquitination		Uniprot
T55	Phosphorylation		Uniprot
T61	Phosphorylation		Uniprot
Y75	Phosphorylation		Uniprot
K81	Ubiquitination		Uniprot
S85	Phosphorylation		Uniprot
K88	Ubiquitination		Uniprot
S89	Phosphorylation		Uniprot
S92	Phosphorylation		Uniprot
T97	Phosphorylation		Uniprot
K104	Acetylation		Uniprot
K104	Ubiquitination		Uniprot
T105	Phosphorylation		Uniprot
Y109	Phosphorylation		Uniprot
S112	Phosphorylation		Uniprot
K115	Acetylation		Uniprot
K115	Ubiquitination		Uniprot
S117	Phosphorylation		Uniprot
K119	Ubiquitination		Uniprot
T123	Phosphorylation		Uniprot
S127	Phosphorylation		Uniprot
C133	S-Nitrosylation		Uniprot
S134	Phosphorylation		Uniprot
T136	Phosphorylation		Uniprot
Y147	Phosphorylation		Uniprot
K148	Acetylation		Uniprot
K148	Ubiquitination		Uniprot
K152	Acetylation		Uniprot
K152	Ubiquitination		Uniprot
K157	Acetylation		Uniprot
K157	Ubiquitination		Uniprot
S161	Phosphorylation		Uniprot
T163	Phosphorylation		Uniprot
S164	Phosphorylation		Uniprot
K169	Methylation		Uniprot
K169	Ubiquitination		Uniprot
K176	Acetylation		Uniprot
K176	Ubiquitination		Uniprot
S184	Phosphorylation		Uniprot
Y188	Phosphorylation		Uniprot
Y199	Phosphorylation		Uniprot
K204	Acetylation		Uniprot
K204	Ubiquitination		Uniprot
K212	Acetylation		Uniprot
K212	Ubiquitination		Uniprot
S215	Phosphorylation		Uniprot
T218	Phosphorylation		Uniprot
S221	Phosphorylation		Uniprot
K227	Acetylation		Uniprot
K227	Methylation		Uniprot
K227	Ubiquitination		Uniprot
Y232	Phosphorylation		Uniprot
K233	Acetylation		Uniprot
K233	Ubiquitination		Uniprot
S234	Phosphorylation		Uniprot
Y235	Phosphorylation		Uniprot
S236	Phosphorylation		Uniprot
Y238	Phosphorylation		Uniprot
S243	Phosphorylation		Uniprot
K246	Ubiquitination		Uniprot
K249	Ubiquitination		Uniprot
C262	S-Nitrosylation		Uniprot
K266	Sumoylation		Uniprot
K266	Ubiquitination		Uniprot
Y269	Phosphorylation		Uniprot
Y275	Phosphorylation		Uniprot
S277	Phosphorylation		Uniprot
K279	Acetylation		Uniprot
K279	Ubiquitination		Uniprot
K281	Ubiquitination		Uniprot
S296	Phosphorylation		Uniprot
K302	Acetylation		Uniprot
K302	Ubiquitination		Uniprot
S305	Phosphorylation		Uniprot
K310	Acetylation		Uniprot
K313	Acetylation		Uniprot
K313	Methylation		Uniprot
K313	Ubiquitination		Uniprot
S314	Phosphorylation		Uniprot
Y316	Phosphorylation		Uniprot
Y317	Phosphorylation		Uniprot
Y318	Phosphorylation		Uniprot
T323	Phosphorylation		Uniprot
K324	Acetylation		Uniprot
K324	Sumoylation		Uniprot
K324	Ubiquitination		Uniprot
K329	Ubiquitination		Uniprot
Y333	Phosphorylation		Uniprot

Research Backgrounds

P07355_ANXA2_HUMAN

Function:

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.

PTMs:

Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.

ISGylated.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix>Basement membrane. Melanosome.
Note: In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.

Subunit Structure:

Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36 (By similarity). Interacts with ATP1B1 (By similarity). Interacts with DYSF (By similarity). Interacts with COCH. Interacts (via repeat Annexin 1) with PCSK9 (via the C-terminal domain); the interaction inhibits the degradation of LDLR. Interacts with CEACAM1 (via the cytoplasmic domain); this interaction is regulated by phosphorylation of CEACAM1. Interacts with APPL2 and APPL1; targets APPL2 to endosomes and acting in parallel to RAB5A (By similarity).

(Microbial infection) Interacts with human cytomegalovirus (HCMV).

Family&Domains:

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Belongs to the annexin family.

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Annexin A2 Antibody - #AF7597