Annexin IV Antibody - #AF7586

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Product: Annexin IV Antibody
Catalog: AF7586
Description: Rabbit polyclonal antibody to Annexin IV
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 36kDa; 36kD(Calculated).
Uniprot: P09525
RRID: AB_2843950

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(90%), Zebrafish(90%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(90%), Chicken(90%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
Annexin IV Antibody detects endogenous levels of total Annexin IV.
RRID:
AB_2843950
Cite Format: Affinity Biosciences Cat# AF7586, RRID:AB_2843950.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

35 beta calcimedin; 35-beta calcimedin; AIV; Annexin 4; Annexin A4; Annexin IV (placental anticoagulant protein II); Annexin IV; Annexin IV placental anticoagulant protein II; Annexin-4; AnnexinA4; AnnexinIV; ANX 4; ANX A4; ANX4; ANXA 4; ANXA4; ANXA4 protein; ANXA4_HUMAN; Carbohydrate binding protein P33/P41; Carbohydrate-binding protein p33/p41; Chromobindin 4; Chromobindin-4; Chromobindin4; DKFZp686H02120; Endonexin I; EndonexinI; Lipocortin IV; LipocortinIV; MGC75105; OTTHUMP00000160052; OTTHUMP00000202207; P32.5; P33/41; PAP II; PAP-II; PAPII; PIG 28; PIG28; Placental anticoagulant protein II; PP4 X; PP4-X; PP4X; Proliferation inducing gene 28; Proliferation inducing protein 28; Protein II; Xanx-4; ZAP 36; ZAP36;

Immunogens

Immunogen:
Uniprot:

P09525(ANXA4_HUMAN) >>Visit HPA database.

Gene(ID):
ANXA4(307)
Sequence:
MATKGGTVKAASGFNAMEDAQTLRKAMKGLGTDEDAIISVLAYRNTAQRQEIRTAYKSTIGRDLIDDLKSELSGNFEQVIVGMMTPTVLYDVQELRRAMKGAGTDEGCLIEILASRTPEEIRRISQTYQQQYGRSLEDDIRSDTSFMFQRVLVSLSAGGRDEGNYLDDALVRQDAQDLYEAGEKKWGTDEVKFLTVLCSRNRNHLLHVFDEYKRISQKDIEQSIKSETSGSFEDALLAIVKCMRNKSAYFAEKLYKSMKGLGTDDNTLIRVMVSRAEIDMLDIRAHFKRLYGKSLYSFIKGDTSGDYRKVLLVLCGGDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Sheep
90
Dog
90
Xenopus
90
Zebrafish
90
Chicken
90
Rabbit
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09525 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S12 Phosphorylation
T22 Phosphorylation
K28 Ubiquitination
K57 Ubiquitination
K69 Sumoylation
C108 S-Nitrosylation
S115 Phosphorylation
S125 Phosphorylation
Y128 Phosphorylation
Y132 Phosphorylation
Y165 Phosphorylation
Y179 Phosphorylation
K184 Ubiquitination
K185 Ubiquitination
S199 Phosphorylation
Y212 Phosphorylation
K213 Acetylation
K218 Ubiquitination
K225 Acetylation
K225 Ubiquitination
K246 Ubiquitination
K253 Acetylation
Y255 Phosphorylation
K259 Acetylation
K259 Ubiquitination
T263 Phosphorylation
T267 Phosphorylation
K293 Acetylation
K293 Ubiquitination
Y296 Phosphorylation
S297 Phosphorylation
K300 Acetylation

Research Backgrounds

Function:

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.

Family&Domains:

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Belongs to the annexin family.

Restrictive clause

 

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