PAX7 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/7

Western blot analysis of extracts from Mouse brain, using PAX7 Antibody.

Western blot analysis of extracts from Mouse testis, using PAX7 Antibody at 1/1000 dilution.

IF/ICC staining HELA cells at 1:100.

AF7584 staining Hela cells by IF/ICC.

Figure 2 Generation of Tmem30a conditional knockout (cKO) mice A: Breeding strategy of cKO mice by crossing Tmem30aloxP/loxP with Pax7CreER mice.

Fig.

Product:	PAX7 Antibody
Catalog:	AF7584
Description:	Rabbit polyclonal antibody to PAX7
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat, Sheep
Prediction:	Pig, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	57kDa; 55kD(Calculated).
Uniprot:	P23759
RRID:	AB_2843948

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Sheep

Prediction:

Pig(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(90%)

Clonality:

Polyclonal

Specificity:

PAX7 Antibody detects endogenous levels of total PAX7.

RRID:

AB_2843948
Cite Format: Affinity Biosciences Cat# AF7584, RRID:AB_2843948.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ37460; HUP1; OTTHUMP00000002534; Paired box 7; Paired box gene 7; Paired box homeotic gene 7; Paired box protein Pax-7; Paired domain gene 7; Paired domain gene HuP1; Pax7; PAX7 transcriptional factor; PAX7/FKHR fusion gene, included; PAX7_HUMAN; PAX7B; RGD1564360; RMS2;

Immunogens

Immunogen:

Uniprot:

P23759(PAX7_HUMAN) >>Visit HPA database.

Gene(ID):

PAX7(5081)

Sequence:

P23759 PAX7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAALPGTVPRMMRPAPGQNYPRTGFPLEVSTPLGQGRVNQLGGVFINGRPLPNHIRHKIVEMAHHGIRPCVISRQLRVSHGCVSKILCRYQETGSIRPGAIGGSKPRQVATPDVEKKIEEYKRENPGMFSWEIRDRLLKDGHCDRSTVPSGLVSSISRVLRIKFGKKEEEDEADKKEDDGEKKAKHSIDGILGDKGNRLDEGSDVESEPDLPLKRKQRRSRTTFTAEQLEELEKAFERTHYPDIYTREELAQRTKLTEARVQVWFSNRRARWRKQAGANQLAAFNHLLPGGFPPTGMPTLPPYQLPDSTYPTTTISQDGGSTVHRPQPLPPSTMHQGGLAAAAAAADTSSAYGARHSFSSYSDSFMNPAAPSNHMNPVSNGLSPQVMSILGNPSAVPPQPQADFSISPLHGGLDSATSISASCSQRADSIKPGDSLPTSQAYCPPTYSTTGYSVDPVAGYQYGQYGQTAVDYLAKNVSLSTQRRMKLGEHSAVLGLLPVETGQAY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Horse

Bovine

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23759 As Substrate

P23759

Site	PTM Type	Source
Y121	Phosphorylation	Uniprot
T147	Phosphorylation	Uniprot
K176	Acetylation	Uniprot
S203	Phosphorylation	Uniprot
S207	Phosphorylation	Uniprot

Research Backgrounds

P23759_PAX7_HUMAN

Function:

Transcription factor playing a role in myogenesis through regulation of muscle precursor cells proliferation.

Subcellular Location:

Nucleus.

Subunit Structure:

Can bind to DNA as a heterodimer with PAX3. Interacts with PAXBP1; the interaction links PAX7 to a WDR5-containing histone methyltransferase complex (By similarity). Interacts with DAXX.

Family&Domains:

Belongs to the paired homeobox family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

References

1). Deletion of phosphatidylserine flippase β-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration. ZOOLOGICAL RESEARCH, 2021 (PubMed: 34472226) [IF=4.9]

Application: IF/ICC Species: Mice Sample: satellite cells (SCs)

Figure 2 Generation of Tmem30a conditional knockout (cKO) mice A: Breeding strategy of cKO mice by crossing Tmem30aloxP/loxP with Pax7CreER mice. B: Genotyping of mice by PCR and agarose gel electrophoresis. C: Axis of tamoxifen intraperitoneal injection, BaCl2 injection, and TA muscle harvesting in mice. D: In 7 dpi cKO mice, expression of Pax7CreER was monitored by ROSA-tdTomato reporter (red). SCs were labeled with Pax7 antibody (green). Nuclei were counterstained with DAPI (blue). Scale bar: 50 μm. E: TMEM30A protein from 7 dpi WT and cKO mice was labeled with TMEM30A antibody (green) and DAPI (blue). Scale bar: 50 μm. F, G: Immunoblot analysis of TMEM30A protein expression in 7 dpi WT and cKO mice. Relative protein band density was calculated by ImageJ. Data are mean±SEM. n=3 in each group. Significance was calculated by two-tailed Student’s t-test. *: P<0.05.

2). Kcnma1 is involved in mitochondrial homeostasis in diabetes-related skeletal muscle atrophy. The FASEB Journal, 2023 (PubMed: 36929614) [IF=4.8]

3). Bi-phasic effect of gelatin in myogenesis and skeletal muscle regeneration. Disease Models & Mechanisms, 2021 (PubMed: 34821368) [IF=4.3]

Application: IF/ICC Species: Mouse Sample: SCs

Fig. 2.Bi-phasic effect of gelatin in activating quiescent satellite cells (SCs) and myogenesis during muscle regeneration. (A) Immunohistochemical (IHC) analysis of Pax7+ SCs (green) in TA muscle stained by laminin (red) at 7 D.P.I. Arrows point to Pax7+ nuclei. Scale bar: 50 μm. (B) The percentage of myofibers containing Pax7+ SCs (n=6). (C) IHC analysis of MyoG+ cells (green) in damaged TA muscle stained by laminin (red) at 7 D.P.I. Arrows point to MyoG+ nuclei. Scale bar: 50 μm. (D) The percentage of myofibers containing MyoG+ cells (n=6). (E,F) Western blots (E) and quantification (F) of MyoD and MyoG in damaged TA muscles at 14 D.P.I. (n=3). GAPDH is used as a loading control. Significance was determined by unpaired two-tailed Student's t-test with Welch's correction.

4). N-Acetylcysteine assists muscle development in offspring of mice subjected to maternal heat stress during pregnancy. Journal of the science of food and agriculture, 2024 (PubMed: 38828636) [IF=4.1]

5). The mechanism of Megalobrama amblycephala muscle injury repair based on RNA-seq. Gene, 2022 (PubMed: 35395368) [IF=3.5]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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PAX7 Antibody - #AF7584