TFAP2C Antibody | Affinity Biosciences

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Western blot analysis of TFAP2C in lysates of MCF7 , using TFAP2C Antibody(AF7582).

Western blot analysis of extracts from Hela cells, using TFAP2C Antibody.

Product:	TFAP2C Antibody
Catalog:	AF7582
Description:	Rabbit polyclonal antibody to TFAP2C
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	49kDa; 49kD(Calculated).
Uniprot:	Q92754
RRID:	AB_2843946

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(90%)

Clonality:

Polyclonal

Specificity:

TFAP2C Antibody detects endogenous levels of total TFAP2C.

RRID:

AB_2843946
Cite Format: Affinity Biosciences Cat# AF7582, RRID:AB_2843946.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Activating enhancer binding protein 2 gamma; Activating enhancer-binding protein 2 gamma; AP2-gamma; AP2C_HUMAN; AP2g; AP2gamma; ERF 1; ERF1; Estrogen receptor factor 1; hAP 2g; hAP2g; TFAP 2C; TFAP 2G; Tfap2c; TFAP2G; Transcription factor AP 2 gamma (activating enhancer binding protein 2 gamma); Transcription factor AP 2 gamma; Transcription factor AP-2 gamma; Transcription factor ERF 1; Transcription factor ERF-1; Transcription factor ERF1;

Immunogens

Immunogen:

Uniprot:

Q92754(AP2C_HUMAN) >>Visit HPA database.

Gene(ID):

TFAP2C(7022)

Sequence:

Q92754 AP2C_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLWKITDNVKYEEDCEDRHDGSSNGNPRVPHLSSAGQHLYSPAPPLSHTGVAEYQPPPYFPPPYQQLAYSQSADPYSHLGEAYAAAINPLHQPAPTGSQQQAWPGRQSQEGAGLPSHHGRPAGLLPHLSGLEAGAVSARRDAYRRSDLLLPHAHALDAAGLAENLGLHDMPHQMDEVQNVDDQHLLLHDQTVIRKGPISMTKNPLNLPCQKELVGAVMNPTEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAAHVTLLTSLVEGEAVHLARDFAYVCEAEFPSKPVAEYLTRPHLGGRNEMAARKNMLLAAQQLCKEFTELLSQDRTPHGTSRLAPVLETNIQNCLSHFSLITHGFGSQAICAAVSALQNYIKEALIVIDKSYMNPGDQSPADSNKTLEKMEKHRK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Sheep

Dog

Rabbit

Chicken

Xenopus

Pig

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92754 As Substrate

Q92754

Site	PTM Type	Source
K10	Sumoylation	Uniprot
K202	Ubiquitination	Uniprot
S236	Phosphorylation	Uniprot
S238	Phosphorylation	Uniprot
K239	Ubiquitination	Uniprot
K241	Ubiquitination	Uniprot
S252	Phosphorylation	Uniprot
K281	Ubiquitination	Uniprot
K284	Ubiquitination	Uniprot
S434	Phosphorylation	Uniprot
S438	Phosphorylation	Uniprot
K444	Ubiquitination	Uniprot
K447	Ubiquitination	Uniprot

Research Backgrounds

Q92754_AP2C_HUMAN

Function:

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

PTMs:

Sumoylated on Lys-10; which inhibits transcriptional activity.

Subcellular Location:

Nucleus.

Subunit Structure:

Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members (By similarity). Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with KCTD1; this interaction represses transcription activation. Interacts with CITED2 (via C-terminus); the interaction stimulates TFAP2B-transcriptional activity. Interacts with MTA1.

Family&Domains:

The PPxY motif mediates interaction with WWOX.

Belongs to the AP-2 family.

Restrictive clause

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For Research Use Only.
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TFAP2C Antibody - #AF7582