TXN Antibody | Affinity Biosciences

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Product:	TXN Antibody
Catalog:	AF7577
Description:	Rabbit polyclonal antibody to TXN
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.:	12kDa; 12kD(Calculated).
Uniprot:	P10599
RRID:	AB_2843941

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(80%), Sheep(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

TXN Antibody detects endogenous levels of total TXN.

RRID:

AB_2843941
Cite Format: Affinity Biosciences Cat# AF7577, RRID:AB_2843941.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ADF; ATL derived factor; ATL-derived factor; DKFZp686B1993; MGC61975; SASP; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; testicular tissue protein Li 199; THIO_HUMAN; Thioredoxin; thioredoxin delta 3; TRDX; TRX 1; Trx; TRX1; TXN; TXN delta 3; TXN protein; zgc:92903;

Immunogens

Immunogen:

Uniprot:

P10599(THIO_HUMAN) >>Visit HPA database.

Gene(ID):

TXN(7295)

Sequence:

P10599 THIO_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVKQIESKTAFQEALDAAGDKLVVVDFSATWCGPCKMIKPFFHSLSEKYSNVIFLEVDVDDCQDVASECEVKCMPTFQFFKKGQKVGEFSGANKEKLEATINELV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Rabbit

100

Horse

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10599 As Substrate

P10599

Site	PTM Type	Enzyme	Source
K3	Acetylation		Uniprot
K3	Ubiquitination		Uniprot
K8	Acetylation		Uniprot
K8	Methylation		Uniprot
K8	Ubiquitination		Uniprot
T9	Phosphorylation		Uniprot
T30	Phosphorylation		Uniprot
K39	Acetylation		Uniprot
K39	Ubiquitination		Uniprot
S44	Phosphorylation		Uniprot
S46	Phosphorylation		Uniprot
K48	Methylation		Uniprot
C62	S-Nitrosylation		Uniprot
S67	Phosphorylation		Uniprot
C69	S-Nitrosylation		Uniprot
C73	S-Nitrosylation		Uniprot
T76	Phosphorylation		Uniprot
K81	Acetylation		Uniprot
K85	Ubiquitination		Uniprot
S90	Phosphorylation		Uniprot
K94	Acetylation		Uniprot
K94	Ubiquitination		Uniprot
K96	Ubiquitination		Uniprot
T100	Phosphorylation	P17252 (PRKCA)	Uniprot

Research Backgrounds

P10599_THIO_HUMAN

Function:

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

PTMs:

In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.

In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.

Subcellular Location:

Nucleus. Cytoplasm. Secreted.
Note: Translocates from the cytoplasm into the nucleus after phorbol 12-myristate 13-acetate induction (PMA) (PubMed:9108029). Predominantly in the cytoplasm in non irradiated cells (PubMed:11118054). Radiation induces translocation of TRX from the cytoplasm to the nucleus (PubMed:11118054). Secreted by a leaderless secretory pathway (PubMed:1332947).

Subunit Structure:

Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner.

Family&Domains:

Belongs to the thioredoxin family.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

References

1). Irisin suppresses pancreatic β cell pyroptosis in T2DM by inhibiting the NLRP3-GSDMD pathway and activating the Nrf2-TrX/TXNIP signaling axis. Diabetology & metabolic syndrome, 2023 (PubMed: 37993958) [IF=4.8]

Application: WB Species: Mouse Sample: Min6 cells

Fig. 3 Irisin decreased oxidative stress levels and pyroptosis through activation of the Nrf2-TrX/TXNIP signaling axis The T2DM cell model was established via HG induction. Then, 100 ng/mL Irisin was added along with the Nrf2 inhibitor ML385, and the level of oxidative stress of cells in each group was evaluated (A: ROS, B: MDA, and C: SOD). (D) Pyroptosis was detected by GSDMD-N staining. (E) WB assays were performed to evaluate the level of expression of Nrf2 in the nucleus, as well as, the level of expression of TXNIP, TrX1, NLRP3, ASC, C-caspase-1, and GSDMD-N. (F) The protein expression levels were calculated as grayscale values. (G) Caspase-1 activity was assessed. ELISA was performed to assess the level of expression of IL-1β and IL-18 proteins (H) for elucidating the mechanism underlying the inhibition of oxidative stress and NLRP3-mediated pyroptosis by irisin. Scale bar = 130 μm

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TXN Antibody - #AF7577