Gli1 Antibody - #AF7571
| Product: | Gli1 Antibody |
| Catalog: | AF7571 |
| Description: | Rabbit polyclonal antibody to Gli1 |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Prediction: | Chicken |
| Mol.Wt.: | 119kDa; 118kD(Calculated). |
| Uniprot: | P08151 |
| RRID: | AB_2843935 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF7571, RRID:AB_2843935.
Fold/Unfold
Gli 1; GLI; GLI family zinc finger 1; GLI Kruppel family member 1; gli1; GLI1_HUMAN; Glioma associated oncogene 1; Glioma associated oncogene homolog 1 (zinc finger protein); Glioma associated oncogene homolog; Glioma-associated oncogene; Oncogene GLI; Zfp 5; Zfp5; Zinc finger protein GLI 1; Zinc finger protein GLI1;
Immunogens
Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761).
- P08151 GLI1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFNSMTPPPISSYGEPCCLRPLPSQGAPSVGTEGLSGPPFCHQANLMSGPHSYGPARETNSCTEGPLFSSPRSAVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPAQMNHQKGPSPSFGVQPCGPHDSARGGMIPHPQSRGPFPTCQLKSELDMLVGKCREEPLEGDMSSPNSTGIQDPLLGMLDGREDLEREEKREPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEHEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAPSISTVEPKREREGGPIREESRLTVPEGAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLENLRLDQLHQLRPIGTRGLKLPSLSHTGTTVSRRVGPPVSLERRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLMPAQHYLLRARYASARGGGTSPTAASSLDRIGGLPMPPWRSRAEYPGYNPNAGVTRRASDPAQAADRPAPARVQRFKSLGCVHTPPTVAGGGQNFDPYLPTSVYSPQPPSITENAAMDARGLQEEPEVGTSMVGSGLNPYMDFPPTDTLGYGGPEGAAAEPYGARGPGSLPLGPGPPTNYGPNPCPQQASYPDPTQETWGEFPSHSGLYPGPKALGGTYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGPPHPQPLFSHYPQPSPPQYLQSGPYTQPPPDYLPSEPRPCLDFDSPTHSTGQLKAQLVCNYVQSQQELLWEGGGREDAPAQEPSYQSPKFLGGSQVSPSRAKAPVNTYGPGFGPNLPNHKSGSYPTPSPCHENFVVGANRASHRAAAPPRLLPPLPTCYGPLKVGGTNPSCGHPEVGRLGGGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEPQGLSPPPSHDQRGSSGHTPPPSGPPNMAVGNMSVLLRSLPGETEFLNSSA
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P08151 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| Ubiquitination | Uniprot | ||
| T63 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | P23443 (RPS6KB1) | Uniprot |
| S102 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| S130 | Phosphorylation | Uniprot | |
| R161 | Methylation | Uniprot | |
| S201 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| T205 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | P41743 (PRKCI) | Uniprot |
| T304 | Phosphorylation | P41743 (PRKCI) | Uniprot |
| K319 | Ubiquitination | Uniprot | |
| K365 | Acetylation | Uniprot | |
| K371 | Acetylation | Uniprot | |
| T374 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S408 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| K518 | Acetylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| T527 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| S544 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S545 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| R597 | Methylation | Uniprot | |
| S602 | Phosphorylation | Uniprot | |
| S622 | Phosphorylation | Uniprot | |
| S640 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S934 | Phosphorylation | Uniprot | |
| S937 | Phosphorylation | Uniprot | |
| S939 | Phosphorylation | Uniprot | |
| S968 | Phosphorylation | Uniprot | |
| S1071 | Phosphorylation | Uniprot | |
| T1074 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| S1078 | Phosphorylation | Uniprot |
Research Backgrounds
Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling.
Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.
Phosphorylated in vitro by ULK3.
Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1.
Cytoplasm. Nucleus.
Note: Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441).
Cytoplasm. Nucleus.
Detected in testis (at protein level). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues.
Interacts with KIF7 (By similarity). Interacts with STK36. Interacts with ZIC1; the interaction enhances transcription activation. Interacts with SUFU; this inhibits transcriptional activation by GLI1.
Belongs to the GLI C2H2-type zinc-finger protein family.
Research Fields
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Basal cell carcinoma. (View pathway)
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