Product: FBP1 Antibody
Catalog: AF7560
Description: Rabbit polyclonal antibody to FBP1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 37kDa; 37kD(Calculated).
Uniprot: P09467
RRID: AB_2843924

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 50ul $250 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Bovine(89%), Horse(89%), Rabbit(100%), Dog(100%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
FBP1 Antibody detects endogenous levels of total FBP1.
RRID:
AB_2843924
Cite Format: Affinity Biosciences Cat# AF7560, RRID:AB_2843924.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

6-bisphosphatase 1; 6-bisphosphate 1-phosphohydrolase 1; D fructose 1 6 bisphosphate 1 phosphohydrolase 1; D-fructose-1; EC 3.1.3.11; F16P1_HUMAN; FBP; FBP 1; FBP1; FBPase 1; Fructose 1 6 bisphosphatase 1; Fructose bisphosphatase 1; Fructose-1; Growth inhibiting protein 17; Liver fructose bisphosphatase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P09467 F16P1_HUMAN:

Expressed in pancreatic islets.

Sequence:
MADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Pig
89
Horse
89
Bovine
89
Chicken
89
Sheep
78
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09467 As Substrate

Site PTM Type Enzyme
T28 Phosphorylation
Y58 Phosphorylation
S63 Phosphorylation
K72 Acetylation
S88 Phosphorylation
S144 Phosphorylation
Y216 Phosphorylation
Y227 Phosphorylation
K232 Acetylation
Y241 Phosphorylation
Y245 Phosphorylation
T256 Phosphorylation
Y259 Phosphorylation
Y265 Phosphorylation
K269 Acetylation
S271 Phosphorylation
T297 Phosphorylation
T298 Phosphorylation
S321 Phosphorylation
K330 Acetylation

Research Backgrounds

Function:

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.

Tissue Specificity:

Expressed in pancreatic islets.

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the FBPase class 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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