KMT5A Antibody - #AF7553
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF7553, RRID:AB_2843917.
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H4 K20 HMTase; H4 K20 specific histone methyltransferase; H4-K20-HMTase SETD8; Histone H4 K20 methyltransferase; Histone lysine N methyltransferase H4 lysine 20 specific; Histone-lysine N-methyltransferase SETD8; Lysine N-methyltransferase 5A; N-lysine methyltransferase SETD8; Pr SET 7; Pr SET7; PR-Set7; PR/SET domain containing protein 07; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; PR/SET07; PrSET7; SET 07; SET 8; SET domain containing (lysine methyltransferase) 8; SET domain containing 8; SET domain containing lysine methyltransferase 8; SET domain containing protein 8; SET domain-containing protein 8; SET07; SET8; SETD 8; setd8; SETD8_HUMAN;
Immunogens
- Q9NQR1 KMT5A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGEGGAAAALVAAAAAAAAAAAAVVAGQRRRRLGRRARCHGPGRAAGGKMSKPCAVEAAAAAVAATAPGPEMVERRGPGRPRTDGENVFTGQSKIYSYMSPNKCSGMRFPLQEENSVTHHEVKCQGKPLAGIYRKREEKRNAGNAVRSAMKSEEQKIKDARKGPLVPFPNQKSEAAEPPKTPPSSCDSTNAAIAKQALKKPIKGKQAPRKKAQGKTQQNRKLTDFYPVRRSSRKSKAELQSEERKRIDELIESGKEEGMKIDLIDGKGRGVIATKQFSRGDFVVEYHGDLIEITDAKKREALYAQDPSTGCYMYYFQYLSKTYCVDATRETNRLGRLINHSKCGNCQTKLHDIDGVPHLILIASRDIAAGEELLYDYGDRSKASIEAHPWLKH
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NQR1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y96 | Phosphorylation | Uniprot | |
| Y98 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K151 | Sumoylation | Uniprot | |
| K162 | Acetylation | Uniprot | |
| K172 | Acetylation | Uniprot | |
| K172 | Sumoylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| K236 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| Y375 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| K392 | Ubiquitination | Uniprot |
Research Backgrounds
Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.
Acetylated at Lys-172; does not change methyltransferase activity. Deacetylated at Lys-172 by SIRT2; does not change methyltransferase activity.
Ubiquitinated and degraded by the DCX(DTL) complex.
Nucleus. Chromosome.
Note: Specifically localizes to mitotic chromosomes. Colocalized with SIRT2 at mitotic foci. Associates with chromosomes during mitosis; association is increased in a H(2)O(2)-induced oxidative stress-dependent manner. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
Interacts with L3MBTL1. Isoform 2 interacts with SIRT2 (phosphorylated form); the interaction is direct, stimulates KMT5A-mediated methyltransferase activity at histone H4 'Lys-20' (H4K20me1) and is increased in a H(2)O(2)-induced oxidative stress-dependent manner.
Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.
Research Fields
· Metabolism > Amino acid metabolism > Lysine degradation.
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