Product: KMT5A Antibody
Catalog: AF7553
Description: Rabbit polyclonal antibody to KMT5A
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 43kDa; 43kD(Calculated).
Uniprot: Q9NQR1
RRID: AB_2843917

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(100%), Sheep(91%), Dog(100%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
KMT5A Antibody detects endogenous levels of total KMT5A.
RRID:
AB_2843917
Cite Format: Affinity Biosciences Cat# AF7553, RRID:AB_2843917.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

H4 K20 HMTase; H4 K20 specific histone methyltransferase; H4-K20-HMTase SETD8; Histone H4 K20 methyltransferase; Histone lysine N methyltransferase H4 lysine 20 specific; Histone-lysine N-methyltransferase SETD8; Lysine N-methyltransferase 5A; N-lysine methyltransferase SETD8; Pr SET 7; Pr SET7; PR-Set7; PR/SET domain containing protein 07; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; PR/SET07; PrSET7; SET 07; SET 8; SET domain containing (lysine methyltransferase) 8; SET domain containing 8; SET domain containing lysine methyltransferase 8; SET domain containing protein 8; SET domain-containing protein 8; SET07; SET8; SETD 8; setd8; SETD8_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MGEGGAAAALVAAAAAAAAAAAAVVAGQRRRRLGRRARCHGPGRAAGGKMSKPCAVEAAAAAVAATAPGPEMVERRGPGRPRTDGENVFTGQSKIYSYMSPNKCSGMRFPLQEENSVTHHEVKCQGKPLAGIYRKREEKRNAGNAVRSAMKSEEQKIKDARKGPLVPFPNQKSEAAEPPKTPPSSCDSTNAAIAKQALKKPIKGKQAPRKKAQGKTQQNRKLTDFYPVRRSSRKSKAELQSEERKRIDELIESGKEEGMKIDLIDGKGRGVIATKQFSRGDFVVEYHGDLIEITDAKKREALYAQDPSTGCYMYYFQYLSKTYCVDATRETNRLGRLINHSKCGNCQTKLHDIDGVPHLILIASRDIAAGEELLYDYGDRSKASIEAHPWLKH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Pig
91
Bovine
91
Sheep
91
Xenopus
89
Chicken
78
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NQR1 As Substrate

Site PTM Type Enzyme
Y96 Phosphorylation
Y98 Phosphorylation
S100 Phosphorylation P06493 (CDK1)
K151 Sumoylation
K162 Acetylation
K172 Acetylation
K172 Sumoylation
T181 Phosphorylation
S184 Phosphorylation
K221 Ubiquitination
S232 Phosphorylation
K236 Ubiquitination
K245 Ubiquitination
S253 Phosphorylation
K255 Ubiquitination
K267 Ubiquitination
K275 Ubiquitination
K342 Ubiquitination
K349 Ubiquitination
Y375 Phosphorylation
S381 Phosphorylation
K382 Ubiquitination
K392 Ubiquitination

Research Backgrounds

Function:

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.

PTMs:

Acetylated at Lys-172; does not change methyltransferase activity. Deacetylated at Lys-172 by SIRT2; does not change methyltransferase activity.

Ubiquitinated and degraded by the DCX(DTL) complex.

Subcellular Location:

Nucleus. Chromosome.
Note: Specifically localizes to mitotic chromosomes. Colocalized with SIRT2 at mitotic foci. Associates with chromosomes during mitosis; association is increased in a H(2)O(2)-induced oxidative stress-dependent manner. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with L3MBTL1. Isoform 2 interacts with SIRT2 (phosphorylated form); the interaction is direct, stimulates KMT5A-mediated methyltransferase activity at histone H4 'Lys-20' (H4K20me1) and is increased in a H(2)O(2)-induced oxidative stress-dependent manner.

Family&Domains:

Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.

Research Fields

· Metabolism > Amino acid metabolism > Lysine degradation.

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