SLC32A1 Antibody - #AF7551

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Product: SLC32A1 Antibody
Catalog: AF7551
Description: Rabbit polyclonal antibody to SLC32A1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 57kDa; 57kD(Calculated).
Uniprot: Q9H598
RRID: AB_2843915

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SLC32A1 Antibody detects endogenous levels of total SLC32A1.
RRID:
AB_2843915
Cite Format: Affinity Biosciences Cat# AF7551, RRID:AB_2843915.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bA122O1.1; GABA and glycine transporter; hVIAAT; SLC32A 1; Slc32a1; solute carrier family 32 (GABA vesicular transporter) member 1; Solute carrier family 32 member 1; Vesicular GABA Amino Acid Transporter; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter; VGAT; VIAAT; VIAAT_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q9H598(VIAAT_HUMAN) >>Visit HPA database.

Gene(ID):
SLC32A1(140679)
Expression:
Q9H598 VIAAT_HUMAN:

Retina. Expressed throughout the horizontal cells or more specifically at the terminals.

Sequence:
MATLLRSKLSNVATSVSNKSQAKMSGMFARMGFQAATDEEAVGFAHCDDLDFEHRQGLQMDILKAEGEPCGDEGAEAPVEGDIHYQRGSGAPLPPSGSKDQVGGGGEFGGHDKPKITAWEAGWNVTNAIQGMFVLGLPYAILHGGYLGLFLIIFAAVVCCYTGKILIACLYEENEDGEVVRVRDSYVAIANACCAPRFPTLGGRVVNVAQIIELVMTCILYVVVSGNLMYNSFPGLPVSQKSWSIIATAVLLPCAFLKNLKAVSKFSLLCTLAHFVINILVIAYCLSRARDWAWEKVKFYIDVKKFPISIGIIVFSYTSQIFLPSLEGNMQQPSEFHCMMNWTHIAACVLKGLFALVAYLTWADETKEVITDNLPGSIRAVVNIFLVAKALLSYPLPFFAAVEVLEKSLFQEGSRAFFPACYSGDGRLKSWGLTLRCALVVFTLLMAIYVPHFALLMGLTGSLTGAGLCFLLPSLFHLRLLWRKLLWHQVFFDVAIFVIGGICSVSGFVHSLEGLIEAYRTNAED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H598 As Substrate

Site PTM Type Enzyme
S17 Phosphorylation
S20 Phosphorylation
S96 Phosphorylation
S98 Phosphorylation
Y300 Phosphorylation
T443 Phosphorylation
S462 Phosphorylation

Research Backgrounds

Function:

Involved in the uptake of GABA and glycine into the synaptic vesicles.

Subcellular Location:

Cytoplasmic vesicle membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Retina. Expressed throughout the horizontal cells or more specifically at the terminals.

Family&Domains:

Belongs to the amino acid/polyamine transporter 2 family.

Research Fields

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > GABAergic synapse.

References

1). Increased Fibronectin Impairs the Function of Excitatory/Inhibitory Synapses in Hirschsprung Disease. CELLULAR AND MOLECULAR NEUROBIOLOGY, 2020 (PubMed: 31760535) [IF=4.0]

Application: WB    Species: rat    Sample: PC12 cell

Fig. 3|Suppression of FN increases the expression of synaptic proteins. A Relative expression of NL-1, PSD-95, vGLUT1, NL-2, SLC32, and FN mRNAs were signifcantly increased in the FN-KD group compared with the NC group and B Western blots of PC12 cell lysates in the FN-KD, NC, and Ctr groups with NL-1, PSD-95, NL-2, SLC32, and FN antibodies. The quantitative results (right) confrm that silencing FN signifcantly increases the expression of neuronal marker proteins

Application: IF/ICC    Species: rat    Sample: PC12 cell

Fig. 3|Suppression of FN increases the expression of synaptic proteins. A Relative expression of NL-1, PSD-95, vGLUT1, NL-2, SLC32, and FN mRNAs were signifcantly increased in the FN-KD group compared with the NC group and B Western blots of PC12 cell lysates in the FN-KD, NC, and Ctr groups with NL-1, PSD-95, NL-2, SLC32, and FN antibodies. The quantitative results (right) confrm that silencing FN signifcantly increases the expression of neuronal marker proteins.C The co-expression of NL-1, or NL-2, or PSD-95, or SLC32 with FN was examined by double immunofuorescence staining. The results support the idea that downregulation of FN enhances NL-1, PSD-95, NL-2, and SLC32 expression. Values are mean ± SEM, *P < 0.05, **P < 0.01, and ***P < 0.001

Application: IHC    Species: human    Sample: ganglionic, transitional, and aganglionic colonic segments

Fig. 2|Quantitative histological evaluations of ganglionic, transitional, and aganglionic colonic segments of HSCR patients. A1–3, a1–3 PSD-95 group, B1–3, b1–3 SLC32 group, C1–3, c1–3 FN group, d histological score, based on the intensity of positive staining (brown), and e area of positive staining shown as a percentage of total tissue area. Figures are representative of at least fve separate experiments. The diferences among the three groups were all statistically signifcant. Values are mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (ganglionic vs. aganglionic, P < 0.0001)

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