TRAF7 Antibody - #AF7546

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Product: TRAF7 Antibody
Catalog: AF7546
Description: Rabbit polyclonal antibody to TRAF7
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 67kD; 75kD(Calculated).
Uniprot: Q6Q0C0
RRID: AB_2843910

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TRAF7 Antibody detects endogenous levels of total TRAF7.
RRID:
AB_2843910
Cite Format: Affinity Biosciences Cat# AF7546, RRID:AB_2843910.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E3 ubiquitin protein ligase TRAF7; E3 ubiquitin-protein ligase TRAF7; RFWD1; Ring finger and WD repeat domain 1; RING finger and WD repeat-containing protein 1; RING finger protein 119; RNF119; TNF receptor associated factor 7; TNF receptor-associated factor 7; TRAF7; TRAF7_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q6Q0C0(TRAF7_HUMAN) >>Visit HPA database.

Gene(ID):
TRAF7(84231)
Expression:
Q6Q0C0 TRAF7_HUMAN:

Ubiquitously expressed with high levels in skeletal muscle, heart, colon, spleen, kidney, liver and placenta.

Sequence:
MSSGKSARYNRFSGGPSNLPTPDVTTGTRMETTFGPAFSAVTTITKADGTSTYKQHCRTPSSSSTLAYSPRDEEDSMPPISTPRRSDSAISVRSLHSESSMSLRSTFSLPEEEEEPEPLVFAEQPSVKLCCQLCCSVFKDPVITTCGHTFCRRCALKSEKCPVDNVKLTVVVNNIAVAEQIGELFIHCRHGCRVAGSGKPPIFEVDPRGCPFTIKLSARKDHEGSCDYRPVRCPNNPSCPPLLRMNLEAHLKECEHIKCPHSKYGCTFIGNQDTYETHLETCRFEGLKEFLQQTDDRFHEMHVALAQKDQEIAFLRSMLGKLSEKIDQLEKSLELKFDVLDENQSKLSEDLMEFRRDASMLNDELSHINARLNMGILGSYDPQQIFKCKGTFVGHQGPVWCLCVYSMGDLLFSGSSDKTIKVWDTCTTYKCQKTLEGHDGIVLALCIQGCKLYSGSADCTIIVWDIQNLQKVNTIRAHDNPVCTLVSSHNVLFSGSLKAIKVWDIVGTELKLKKELTGLNHWVRALVAAQSYLYSGSYQTIKIWDIRTLDCIHVLQTSGGSVYSIAVTNHHIVCGTYENLIHVWDIESKEQVRTLTGHVGTVYALAVISTPDQTKVFSASYDRSLRVWSMDNMICTQTLLRHQGSVTALAVSRGRLFSGAVDSTVKVWTC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6Q0C0 As Substrate

Site PTM Type Enzyme
Y9 Phosphorylation
S13 Phosphorylation
S17 Phosphorylation
T21 Phosphorylation
Y53 Phosphorylation
K54 Ubiquitination
T59 Phosphorylation
S61 Phosphorylation
S62 Phosphorylation
S63 Phosphorylation
S64 Phosphorylation
T65 Phosphorylation
S69 Phosphorylation
S76 Phosphorylation
S81 Phosphorylation
T82 Phosphorylation
S86 Phosphorylation
S88 Phosphorylation
S91 Phosphorylation
S94 Phosphorylation
S97 Phosphorylation
S105 Phosphorylation
S108 Phosphorylation
T144 Phosphorylation
T149 Phosphorylation
K160 Ubiquitination
S197 Phosphorylation
K199 Ubiquitination
K220 Ubiquitination
K252 Ubiquitination
K288 Ubiquitination
K308 Ubiquitination
K321 Ubiquitination
K325 Ubiquitination
K331 Ubiquitination
K346 Ubiquitination
K387 Methylation
K387 Ubiquitination
K430 Ubiquitination
K511 Ubiquitination
S658 Phosphorylation

Research Backgrounds

Function:

E3 ubiquitin ligase capable of auto-ubiquitination, following phosphorylation by MAP3K3. Potentiates MAP3K3-mediated activation of the NF-kappa-B, JUN/AP1 and DDIT3 transcriptional regulators. Induces apoptosis when overexpressed. Plays a role in the phosphorylation of MAPK1 and/or MAPK3, probably via its interaction with MAP3K3.

PTMs:

Phosphorylated by MAP3K3.

Ubiquitinates itself upon phosphorylation.

Subcellular Location:

Cytoplasmic vesicle.
Note: Colocalizes with MAP3K3 to vesicle-like structures throughout the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed with high levels in skeletal muscle, heart, colon, spleen, kidney, liver and placenta.

Subunit Structure:

Homodimer. Interacts with MAP3K3 and promotes the kinase activity of this enzyme.

Family&Domains:

Belongs to the WD repeat TRAF7 family.

Restrictive clause

 

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