HNF1A Antibody | Affinity Biosciences

WB
Cites

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Western blot analysis of HNF1A in lysates of HepG2 , using HNF1A Antibody(AF7545).

Western blot analysis of extracts from RAW264.

Fig.

FIGURE 6| A, Original blots for HNF‐1, GSTA1 and β‐actin.

Product:	HNF1A Antibody
Catalog:	AF7545
Description:	Rabbit polyclonal antibody to HNF1A
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	56kDa, 70 kDa; 67kD(Calculated).
Uniprot:	P20823
RRID:	AB_2843909

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

HNF1A Antibody detects endogenous levels of total HNF1A.

RRID:

AB_2843909
Cite Format: Affinity Biosciences Cat# AF7545, RRID:AB_2843909.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Albumin proximal factor; Hepatic nuclear factor 1 alpha; Hepatic nuclear factor 1; Hepatic transcription factor 1 alpha; Hepatic transcription factor 1; Hepatocyte nuclear factor 1-alpha; HNF 1; HNF 1A; HNF-1-alpha; HNF-1A; hnf1a; HNF1A_HUMAN; Interferon production regulator factor; LF B1; LF B1 hepatic nuclear factor; LFB 1; LFB1; LFB1 hepatic nuclear factor; Liver specific transcription factor LF B1; Liver specific transcription factor LFB1; Liver-specific transcription factor LF-B1; Maturity onset diabetes of the young 3; MODY 3; MODY3; TCF 1; TCF-1; TCF1; Transcription factor 1; Transcription factor 1 hepatic;

Immunogens

Immunogen:

Uniprot:

P20823(HNF1A_HUMAN) >>Visit HPA database.

Gene(ID):

HNF1A(6927)

Expression:

P20823 HNF1A_HUMAN:

Liver.

Sequence:

P20823 HNF1A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVSKLSQLQTELLAALLESGLSKEALIQALGEPGPYLLAGEGPLDKGESCGGGRGELAELPNGLGETRGSEDETDDDGEDFTPPILKELENLSPEEAAHQKAVVETLLQEDPWRVAKMVKSYLQQHNIPQREVVDTTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREVAQQFTHAGQGGLIEEPTGDELPTKKGRRNRFKWGPASQQILFQAYERQKNPSKEERETLVEECNRAECIQRGVSPSQAQGLGSNLVTEVRVYNWFANRRKEEAFRHKLAMDTYSGPPPGPGPGPALPAHSSPGLPPPALSPSKVHGVRYGQPATSETAEVPSSSGGPLVTVSTPLHQVSPTGLEPSHSLLSTEAKLVSAAGGPLPPVSTLTALHSLEQTSPGLNQQPQNLIMASLPGVMTIGPGEPASLGPTFTNTGASTLVIGLASTQAQSVPVINSMGSSLTTLQPVQFSQPLHPSYQQPLMPPVQSHVTQSPFMATMAQLQSPHALYSHKPEVAQYTHTGLLPQTMLITDTTNLSALASLTPTKQVFTSDTEASSESGLHTPASQATTLHVPSQDPAGIQHLQPAHRLSASPTVSSSSLVLYQSSDSSNGQSHLLPSNHSVIETFISTQMASSSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Pig

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20823 As Substrate

P20823

Site	PTM Type	Enzyme	Source
T67	Phosphorylation		Uniprot
S70	Phosphorylation		Uniprot
T74	Phosphorylation		Uniprot
T82	Phosphorylation		Uniprot
S93	Phosphorylation		Uniprot
T196	Phosphorylation		Uniprot
S247	Phosphorylation		Uniprot
S249	Phosphorylation	Q9Y463 (DYRK1B) , Q13315 (ATM)	Uniprot

Research Backgrounds

P20823_HNF1A_HUMAN

Function:

Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).

Subcellular Location:

Nucleus.

Tissue Specificity:

Liver.

Subunit Structure:

Binds DNA as a dimer. Heterotetramer with PCBD1; formed by a dimer of dimers (By similarity). Interacts with PCBD1. Interacts with BHLHE41 (By similarity).

Family&Domains:

Belongs to the HNF1 homeobox family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

References

1). Acetaminophen-induced hepatocyte injury: C2-ceramide and oltipraz intervention, hepatocyte nuclear factor 1 and glutathione S-transferase A1 changes. JOURNAL OF APPLIED TOXICOLOGY (PubMed: 31385618) [IF=3.3]

Application: WB Species: Sample: hepatocytes

FIGURE 6| A, Original blots for HNF‐1, GSTA1 and β‐actin. B, HNF‐1 protein level. C, GSTA1 protein level. Changes in HNF‐1 and GSTA1 protein levels in hepatocytes after 15 mmol/L APAP exposure for 10 h with C2 (6 μmol/L) or OL (8 μmol/L). Values are expressed as the mean ± SD for each group (n = 4). *P < .05, **P < .01 represented all groups compared with the control group; #P < .05, ##P < .01 represented all groups compared with the APAP model group. APAP, acetaminophen; C2, C2‐ceramide; GSTA1, glutathione S‐transferase A1; HNF‐1, hepatocyte nuclear factor 1; OL, oltipraz

2). Acetaminophen-induced reduction in glutathione-S-transferase A1 in hepatocytes: A role for hepatic nuclear factor 1α and its response element. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (PubMed: 31230750) [IF=3.1]

Application: WB Species: human Sample: HepG2 cells

Fig. 3. |Effects of C2-ceramide or oltipraz on the nuclear HNF-1a levels and the GSTA1 protein expression in HepG2 cells with injury induced by APAP (n ¼ 4). *P < 0.05, **P < 0.01 compared with control group; #P < 0.05, ##P < 0.01 compared with model group.

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HNF1A Antibody - #AF7545