Product: HNF1A Antibody
Catalog: AF7545
Source: Rabbit
Application: WB, ELISA(peptide)
Reactivity: Human, Mouse
Prediction: Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 56kD, 70 kD; 67kD(Calculated).
Uniprot: P20823
RRID: AB_2843909

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
HNF1A Antibody detects endogenous levels of total HNF1A.
RRID:
AB_2843909
Cite Format: Affinity Biosciences Cat# AF7545, RRID:AB_2843909.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Albumin proximal factor; Hepatic nuclear factor 1 alpha; Hepatic nuclear factor 1; Hepatic transcription factor 1 alpha; Hepatic transcription factor 1; Hepatocyte nuclear factor 1-alpha; HNF 1; HNF 1A; HNF-1-alpha; HNF-1A; hnf1a; HNF1A_HUMAN; Interferon production regulator factor; LF B1; LF B1 hepatic nuclear factor; LFB 1; LFB1; LFB1 hepatic nuclear factor; Liver specific transcription factor LF B1; Liver specific transcription factor LFB1; Liver-specific transcription factor LF-B1; Maturity onset diabetes of the young 3; MODY 3; MODY3; TCF 1; TCF-1; TCF1; Transcription factor 1; Transcription factor 1 hepatic;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
MVSKLSQLQTELLAALLESGLSKEALIQALGEPGPYLLAGEGPLDKGESCGGGRGELAELPNGLGETRGSEDETDDDGEDFTPPILKELENLSPEEAAHQKAVVETLLQEDPWRVAKMVKSYLQQHNIPQREVVDTTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREVAQQFTHAGQGGLIEEPTGDELPTKKGRRNRFKWGPASQQILFQAYERQKNPSKEERETLVEECNRAECIQRGVSPSQAQGLGSNLVTEVRVYNWFANRRKEEAFRHKLAMDTYSGPPPGPGPGPALPAHSSPGLPPPALSPSKVHGVRYGQPATSETAEVPSSSGGPLVTVSTPLHQVSPTGLEPSHSLLSTEAKLVSAAGGPLPPVSTLTALHSLEQTSPGLNQQPQNLIMASLPGVMTIGPGEPASLGPTFTNTGASTLVIGLASTQAQSVPVINSMGSSLTTLQPVQFSQPLHPSYQQPLMPPVQSHVTQSPFMATMAQLQSPHALYSHKPEVAQYTHTGLLPQTMLITDTTNLSALASLTPTKQVFTSDTEASSESGLHTPASQATTLHVPSQDPAGIQHLQPAHRLSASPTVSSSSLVLYQSSDSSNGQSHLLPSNHSVIETFISTQMASSSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Pig
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20823 As Substrate

Site PTM Type Enzyme
T67 Phosphorylation
S70 Phosphorylation
T74 Phosphorylation
T82 Phosphorylation
S93 Phosphorylation
T196 Phosphorylation
S247 Phosphorylation
S249 Phosphorylation Q9Y463 (DYRK1B) , Q13315 (ATM)

Research Backgrounds

Function:

Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver (By similarity). Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Activates the transcription of CYP1A2, CYP2E1 and CYP3A11 (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Liver.

Subunit Structure:

Binds DNA as a dimer. Heterotetramer with PCBD1; formed by a dimer of dimers (By similarity). Interacts with PCBD1. Interacts with BHLHE41 (By similarity).

Family&Domains:

Belongs to the HNF1 homeobox family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

References

1). Chang Y et al. Acetaminophen-induced hepatocyte injury: C2-ceramide and oltipraz intervention, hepatocyte nuclear factor 1 and glutathione S-transferase A1 changes. J Appl Toxicol 2019 Dec;39(12):1640-1650 (PubMed: 31385618) [IF=3.628]

Application: WB    Species:    Sample: hepatocytes

FIGURE 6| A, Original blots for HNF‐1, GSTA1 and β‐actin. B, HNF‐1 protein level. C, GSTA1 protein level. Changes in HNF‐1 and GSTA1 protein levels in hepatocytes after 15 mmol/L APAP exposure for 10 h with C2 (6 μmol/L) or OL (8 μmol/L). Values are expressed as the mean ± SD for each group (n = 4). *P < .05, **P < .01 represented all groups compared with the control group; #P < .05, ##P < .01 represented all groups compared with the APAP model group. APAP, acetaminophen; C2, C2‐ceramide; GSTA1, glutathione S‐transferase A1; HNF‐1, hepatocyte nuclear factor 1; OL, oltipraz

2). Li Y et al. Acetaminophen-induced reduction in glutathione-S-transferase A1 in hepatocytes: A role for hepatic nuclear factor 1α and its response element. Biochem Biophys Res Commun 2019 Aug 13;516(1):251-257 (PubMed: 31230750) [IF=3.322]

Application: WB    Species: human    Sample: HepG2 cells

Fig. 3. |Effects of C2-ceramide or oltipraz on the nuclear HNF-1a levels and the GSTA1 protein expression in HepG2 cells with injury induced by APAP (n ¼ 4). *P < 0.05, **P < 0.01 compared with control group; #P < 0.05, ##P < 0.01 compared with model group.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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