CUL4A Antibody - #AF7538

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Product: CUL4A Antibody
Catalog: AF7538
Description: Rabbit polyclonal antibody to CUL4A
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Rabbit, Dog
Mol.Wt.: 88kDa; 88kD(Calculated).
Uniprot: Q13619
RRID: AB_2843902

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(86%), Rabbit(86%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CUL4A Antibody detects endogenous levels of total CUL4A.
RRID:
AB_2843902
Cite Format: Affinity Biosciences Cat# AF7538, RRID:AB_2843902.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2810470J21Rik; AW495282; CUL 4A; CUL-4A; Cul4a; Cul4a protein; CUL4A_HUMAN; Cullin-4A; MGC36573; MGC64071;

Immunogens

Immunogen:
Uniprot:

Q13619(CUL4A_HUMAN) >>Visit HPA database.

Gene(ID):
CUL4A(8451)
Sequence:
MADEAPRKGSFSALVGRTNGLTKPAALAAAPAKPGGAGGSKKLVIKNFRDRPRLPDNYTQDTWRKLHEAVRAVQSSTSIRYNLEELYQAVENLCSHKVSPMLYKQLRQACEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSTLPSIWDMGLELFRTHIISDKMVQSKTIDGILLLIERERSGEAVDRSLLRSLLGMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVITYLDHSTQKPLIACVEKQLLGEHLTAILQKGLDHLLDENRVPDLAQMYQLFSRVRGGQQALLQHWSEYIKTFGTAIVINPEKDKDMVQDLLDFKDKVDHVIEVCFQKNERFVNLMKESFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERTLDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSDSGPIDLTVNILTMGYWPTYTPMEVHLTPEMIKLQEVFKAFYLGKHSGRKLQWQTTLGHAVLKAEFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNGEFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLIDRDYMERDKDNPNQYHYVA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
86
Rabbit
86
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13619 As Substrate

Site PTM Type Enzyme
K8 Ubiquitination
S10 Phosphorylation
S12 Phosphorylation
T22 Phosphorylation
K23 Acetylation
K33 Ubiquitination
S40 Phosphorylation
K41 Acetylation
K42 Acetylation
S99 Phosphorylation
K104 Ubiquitination
K133 Ubiquitination
S151 Phosphorylation
Y227 Phosphorylation
K234 Ubiquitination
Y259 Phosphorylation
K265 Ubiquitination
K284 Ubiquitination
R330 Methylation
K357 Ubiquitination
K359 Ubiquitination
K369 Ubiquitination
K391 Ubiquitination
K404 Ubiquitination
K411 Ubiquitination
K416 Ubiquitination
K422 Acetylation
K422 Ubiquitination
T425 Phosphorylation
Y453 Phosphorylation
K454 Ubiquitination
K465 Ubiquitination
K480 Ubiquitination
K490 Ubiquitination
K496 Ubiquitination
K502 Ubiquitination
K555 Acetylation
K561 Ubiquitination
C633 S-Nitrosylation
K635 Ubiquitination
S642 Phosphorylation
K646 Ubiquitination
K661 Ubiquitination
R692 Methylation
R702 Methylation
K705 Ubiquitination
K708 Ubiquitination
K724 Ubiquitination
K728 Ubiquitination
K733 Ubiquitination
S738 Phosphorylation
R742 Methylation
Y744 Phosphorylation
K749 Ubiquitination

Research Backgrounds

Function:

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL. The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1. With CUL4B, contributes to ribosome biogenesis.

PTMs:

Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.

(Microbial infection) Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.

Subunit Structure:

Component of multiple DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes that seem to consist of DDB1, CUL4A or CUL4B, RBX1 and a variable substrate recognition component which seems to belong to a protein family described as DCAF (Ddb1- and Cul4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Component of the CSA complex (DCX(ERCC8) complex) containing ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II. Component of the DCX(DET1-COP1) complex with the substrate recognition component DET1 and COP1. Component of the DCX(DDB2) complex with the substrate recognition component DDB2. Component of the DCX(DTL) complex with the putative substrate recognition component DTL. Interacts with DDB1, RBX1, RNF7, CTD1, TIP120A/CAND1, SKP2, CDKN1B, MDM2, TP53 and HOXA9. Interacts with DDB2; the interactions with DDB2 and CAND1 are mutually exclusive. Interacts with DCAF1, DTL, DDA1, DCAF6, DCAF4, DCAF16, DCAF17, DET1, WDTC1, DCAF5, DCAF11, WDR24A, COP1, PAFAH1B1, ERCC8, GRWD1, FBXW5, RBBP7, GNB2, WSB1, WSB2, NUP43, PWP1, FBXW8, ATG16L1, KATNB1, RBBP4, RBBP5, LRWD1 and DCAF8. May interact with WDR26, WDR51B, SNRNP40, WDR61, WDR76, WDR5. Can self-associate. Interacts (when neddylated) with ARIH1; leading to activate the E3 ligase activity of ARIH1. The DDB1-CUL4A complex interacts with CRY1.

(Microbial infection) Interacts with Epstein-Barr virus BPLF1.

Family&Domains:

Belongs to the cullin family.

Research Fields

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

References

1). CUL4A promotes cell invasion in gastric cancer by activating the NF-κB signaling pathway. Biologics: Targets and Therapy, 2017 (PubMed: 28442889) [IF=4.0]

Application: WB    Species: human    Sample: HGC27

Figure 4 CUL4A and NF-κB were overexpressed in GC tissues. Notes: (A) Representative images of gastric tumor tissues showing concordant positive staining of CUL4A and NF-κB in the same sample (200×). (B) Western blot analysis of CUL4A and NF-κB expressions in three paired primary GC and adjacent noncancerous tissue samples. These three patients were all diagnosed stage III. (C) CUL4A expression scores and NF-κB expression scores in 50 GC samples revealed that CUL4A expression positively correlated with NF-κB expression via correlation analysis

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