Phospho-KIF5B (Ser154) Antibody | Affinity Biosciences

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Product:	Phospho-KIF5B (Ser154) Antibody
Catalog:	AF7447
Description:	Rabbit polyclonal antibody to Phospho-KIF5B (Ser154)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	110kDa; 110kD(Calculated).
Uniprot:	P33176
RRID:	AB_2843887

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-KIF5B (Ser154) Antibody detects endogenous levels of KIF5B only when phosphorylated at Ser154.

RRID:

AB_2843887
Cite Format: Affinity Biosciences Cat# AF7447, RRID:AB_2843887.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Conventional kinesin heavy chain; KIF 5B; KIF5B; Kinesin 1; kinesin 1 (110-120kD); Kinesin 1 heavy chain; Kinesin family member 5B; Kinesin heavy chain; kinesin, heavy chain, ubiquitous; Kinesin-1 heavy chain; Kinesin1; KINH; KINH_HUMAN; KNS 1; KNS; KNS1; Ubiquitous kinesin heavy chain; UKHC;

Immunogens

Immunogen:

Uniprot:

P33176(KINH_HUMAN) >>Visit HPA database.

Gene(ID):

KIF5B(3799)

Sequence:

P33176 KINH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEIDSDDTGGSAAQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKNMARRGHSAQIAKPIRPGQHPAASPTHPSAIRGGGAFVQNSQPVAVRGGGGKQV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Pig

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P33176 As Substrate

P33176

Site	PTM Type	Source
A2	Acetylation	Uniprot
K10	Acetylation	Uniprot
K10	Ubiquitination	Uniprot
S21	Phosphorylation	Uniprot
K28	Acetylation	Uniprot
S43	Phosphorylation	Uniprot
K44	Ubiquitination	Uniprot
S55	Phosphorylation	Uniprot
C65	S-Nitrosylation	Uniprot
K67	Ubiquitination	Uniprot
K150	Ubiquitination	Uniprot
S154	Phosphorylation	Uniprot
K159	Ubiquitination	Uniprot
K166	Acetylation	Uniprot
S175	Phosphorylation	Uniprot
S206	Phosphorylation	Uniprot
K222	Ubiquitination	Uniprot
K226	Ubiquitination	Uniprot
K240	Ubiquitination	Uniprot
K252	Ubiquitination	Uniprot
Y277	Phosphorylation	Uniprot
R321	Methylation	Uniprot
K346	Acetylation	Uniprot
K382	Ubiquitination	Uniprot
K384	Ubiquitination	Uniprot
K394	Ubiquitination	Uniprot
T397	Phosphorylation	Uniprot
T399	Phosphorylation	Uniprot
K402	Ubiquitination	Uniprot
T405	Phosphorylation	Uniprot
T414	Phosphorylation	Uniprot
K420	Ubiquitination	Uniprot
K427	Ubiquitination	Uniprot
K430	Ubiquitination	Uniprot
K448	Ubiquitination	Uniprot
T451	Phosphorylation	Uniprot
T463	Phosphorylation	Uniprot
R464	Methylation	Uniprot
K505	Ubiquitination	Uniprot
K514	Ubiquitination	Uniprot
Y516	Phosphorylation	Uniprot
K526	Ubiquitination	Uniprot
K539	Ubiquitination	Uniprot
K549	Acetylation	Uniprot
K560	Acetylation	Uniprot
Y593	Phosphorylation	Uniprot
S599	Phosphorylation	Uniprot
K602	Acetylation	Uniprot
K609	Acetylation	Uniprot
K609	Ubiquitination	Uniprot
C632	S-Nitrosylation	Uniprot
K644	Ubiquitination	Uniprot
S645	Phosphorylation	Uniprot
Y649	Phosphorylation	Uniprot
K656	Ubiquitination	Uniprot
K657	Ubiquitination	Uniprot
S663	Phosphorylation	Uniprot
K679	Ubiquitination	Uniprot
K690	Ubiquitination	Uniprot
K698	Ubiquitination	Uniprot
K761	Acetylation	Uniprot
K764	Acetylation	Uniprot
K764	Ubiquitination	Uniprot
K783	Ubiquitination	Uniprot
K791	Ubiquitination	Uniprot
K801	Ubiquitination	Uniprot
S814	Phosphorylation	Uniprot
S819	Phosphorylation	Uniprot
S825	Phosphorylation	Uniprot
S833	Phosphorylation	Uniprot
K844	Ubiquitination	Uniprot
K847	Ubiquitination	Uniprot
R901	Methylation	Uniprot
S917	Phosphorylation	Uniprot
K922	Ubiquitination	Uniprot
S933	Phosphorylation	Uniprot
T935	Phosphorylation	Uniprot
S938	Phosphorylation	Uniprot
S950	Phosphorylation	Uniprot
R956	Methylation	Uniprot

Research Backgrounds

P33176_KINH_HUMAN

Function:

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton.
Note: Uniformly distributed between soma and neurites in hippocampal neurons.

Subunit Structure:

Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 and PPP1R42 (By similarity). Interacts with SYBU. Interacts with JAKMIP1. Interacts with PLEKHM2. Interacts with ECPAS. Interacts with ZFYVE27 (By similarity). Found in a complex with OGT, RHOT1, RHOT2 and TRAK1. Interacts with APP (via cytoplasmic domain).

Family&Domains:

Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

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Phospho-KIF5B (Ser154) Antibody - #AF7447