Phospho-Myt1 (Ser120) Antibody - #AF7435
| Product: | Phospho-Myt1 (Ser120) Antibody |
| Catalog: | AF7435 |
| Description: | Rabbit polyclonal antibody to Phospho-Myt1 (Ser120) |
| Application: | WB |
| Reactivity: | Human |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 70kDa; 55kD(Calculated). |
| Uniprot: | Q99640 |
| RRID: | AB_2843875 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF7435, RRID:AB_2843875.
Fold/Unfold
cdc2 inhibitory kinase; DKFZp547K1610; FLJ20093; Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; MYT1; Myt1 kinase; PKMYT 1; Pkmyt1; PMYT1_HUMAN; PPP1R126; Protein kinase membrane associated tyrosine/threonine 1; Protein kinase Myt1; Protein phosphatase 1 regulatory subunit 126;
Immunogens
- Q99640 PMYT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLERPPALAMPMPTEGTPPPLSGTPIPVPAYFRHAEPGFSLKRPRGLSRSLPPPPPAKGSIPISRLFPPRTPGWHQLQPRRVSFRGEASETLQSPGYDPSRPESFFQQSFQRLSRLGHGSYGEVFKVRSKEDGRLYAVKRSMSPFRGPKDRARKLAEVGSHEKVGQHPCCVRLEQAWEEGGILYLQTELCGPSLQQHCEAWGASLPEAQVWGYLRDTLLALAHLHSQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGTAGAGEVQEGDPRYMAPELLQGSYGTAADVFSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRSVLVMMLEPDPKLRATAEALLALPVLRQPRAWGVLWCMAAEALSRGWALWQALLALLCWLWHGLAHPASWLQPLGPPATPPGSPPCSLLLDSSLSSNWDDDSLGPSLSPEAVLARTVGSTSTPRSRCTPRDALDLSDINSEPPRGSFPSFEPRNLLSLFEDTLDPT
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99640 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| M1 | Acetylation | Uniprot | |
| T17 | Phosphorylation | Uniprot | |
| T24 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| K42 | Methylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | P31749 (AKT1) , P45983 (MAPK8) | Uniprot |
| S89 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| Y97 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| K126 | Methylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| T412 | Phosphorylation | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| S426 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S441 | Phosphorylation | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| T461 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| S482 | Phosphorylation | Uniprot | |
| T495 | Phosphorylation | P53350 (PLK1) | Uniprot |
Research Backgrounds
Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.
Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.
Endoplasmic reticulum membrane>Peripheral membrane protein. Golgi apparatus membrane>Peripheral membrane protein.
Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1.
The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Research Fields
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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