Phospho-SGK3 (Ser486) Antibody - #AF7430
| Product: | Phospho-SGK3 (Ser486) Antibody |
| Catalog: | AF7430 |
| Description: | Rabbit polyclonal antibody to Phospho-SGK3 (Ser486) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 50-60kDa; 57kD(Calculated). |
| Uniprot: | Q96BR1 |
| RRID: | AB_2843870 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF7430, RRID:AB_2843870.
Fold/Unfold
CISK; Cytokine independent survival kinase; DKFZp781N0293; Serine/threonine protein kinase Sgk3; Serine/threonine-protein kinase Sgk3; Serum/glucocorticoid regulated kinase 3; Serum/glucocorticoid regulated kinase family member 3; Serum/glucocorticoid regulated kinase like; Serum/glucocorticoid-regulated kinase 3; Serum/glucocorticoid-regulated kinase-like; SGK 2; SGK 3; SGK2; Sgk3; SGK3_HUMAN; SGKL;
Immunogens
Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. Expression is higher in ER-positive breast tumors than ER-negative breast tumors.
- Q96BR1 SGK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQRDHTMDYKESCPSVSIPSSDEHREKKKRFTVYKVLVSVGRSEWFVFRRYAEFDKLYNTLKKQFPAMALKIPAKRIFGDNFDPDFIKQRRAGLNEFIQNLVRYPELYNHPDVRAFLQMDSPKHQSDPSEDEDERSSQKLHSTSQNINLGPSGNPHAKPTDFDFLKVIGKGSFGKVLLAKRKLDGKFYAVKVLQKKIVLNRKEQKHIMAERNVLLKNVKHPFLVGLHYSFQTTEKLYFVLDFVNGGELFFHLQRERSFPEHRARFYAAEIASALGYLHSIKIVYRDLKPENILLDSVGHVVLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQPYDNTVDWWCLGAVLYEMLYGLPPFYCRDVAEMYDNILHKPLSLRPGVSLTAWSILEELLEKDRQNRLGAKEDFLEIQNHPFFESLSWADLVQKKIPPPFNPNVAGPDDIRNFDTAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSYAPPSEDLFL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96BR1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T32 | Phosphorylation | Uniprot | |
| K75 | Methylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| Y188 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| T318 | Phosphorylation | Uniprot | |
| T320 | Phosphorylation | O15530 (PDPK1) | Uniprot |
| T324 | Phosphorylation | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| S486 | Phosphorylation | Uniprot |
PTMs - Q96BR1 As Enzyme
Research Backgrounds
Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.
Activated by phosphorylation on Ser-486 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-320.
Cytoplasmic vesicle. Early endosome. Recycling endosome.
Note: Endosomal localization is a prerequisite for complete kinase activity. It is essential for its colocalization with the kinase responsible for phosphorylating Ser-486 thus allowing PDPK1 phosphorylation of Thr-320 resulting in complete activation of SGK3. Localized in vesicle-like structures and in the early endosome. Colocalizes with SLC9A3/NHE3 in the recycling endosomes.
Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. Expression is higher in ER-positive breast tumors than ER-negative breast tumors.
Interacts with GSK3B and FLII. Interacts with PDPK1 in a phosphorylation-dependent manner.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Research Fields
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
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