Phospho-MLKL (Ser358) Antibody | Affinity Biosciences

Product:	Phospho-MLKL (Ser358) Antibody
Catalog:	AF7420
Description:	Rabbit polyclonal antibody to Phospho-MLKL (Ser358)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	54kDa; 54kD(Calculated).
Uniprot:	Q8NB16
RRID:	AB_2843860

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-MLKL (Ser358) Antibody detects endogenous levels of MLKL only when phosphorylated at Ser358.

RRID:

AB_2843860
Cite Format: Affinity Biosciences Cat# AF7420, RRID:AB_2843860.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

9130019I15Rik; FLJ34389; hMLKL; Mixed lineage kinase domain like; Mixed lineage kinase domain like protein; Mixed lineage kinase domain like pseudokinase; Mixed lineage kinase domain-like protein; Mlkl; MLKL_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q8NB16(MLKL_HUMAN) >>Visit HPA database.

Gene(ID):

MLKL(197259)

Sequence:

Q8NB16 MLKL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQYLPPKCMQEIPQEQIKEIKKEQLSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGICIDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGFELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK

PTMs - Q8NB16 As Substrate

Q8NB16

Site	PTM Type	Enzyme	Source
K40	Ubiquitination		Uniprot
K50	Ubiquitination		Uniprot
S52	Phosphorylation		Uniprot
K57	Ubiquitination		Uniprot
T59	Phosphorylation		Uniprot
K66	Ubiquitination		Uniprot
K78	Ubiquitination		Uniprot
S92	Phosphorylation		Uniprot
S106	Phosphorylation		Uniprot
S125	Phosphorylation		Uniprot
S128	Phosphorylation		Uniprot
K157	Ubiquitination		Uniprot
S161	Phosphorylation		Uniprot
K173	Ubiquitination		Uniprot
K183	Ubiquitination		Uniprot
K198	Ubiquitination		Uniprot
K219	Ubiquitination		Uniprot
K230	Ubiquitination		Uniprot
T246	Phosphorylation		Uniprot
K249	Ubiquitination		Uniprot
T302	Phosphorylation		Uniprot
K331	Ubiquitination		Uniprot
S334	Phosphorylation		Uniprot
K354	Acetylation		Uniprot
K354	Ubiquitination		Uniprot
T357	Phosphorylation	Q9Y572 (RIPK3)	Uniprot
S358	Phosphorylation	Q9Y572 (RIPK3)	Uniprot
T364	Phosphorylation		Uniprot
K372	Ubiquitination		Uniprot
S373	Phosphorylation		Uniprot
T374	Phosphorylation		Uniprot
S393	Phosphorylation		Uniprot
S417	Phosphorylation		Uniprot
S467	Phosphorylation		Uniprot

Research Backgrounds

Q8NB16_MLKL_HUMAN

Function:

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function.

PTMs:

Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane.

Subcellular Location:

Cytoplasm. Cell membrane.
Note: Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.

Subunit Structure:

Homooligomer. Homotrimer; forms homotrimers on necroptosis induction. Interacts with RIPK3; the interaction is direct. Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5.

Family&Domains:

The protein kinase domain is catalytically inactive but contains an unusual pseudoactive site with an interaction between Lys-230 and Gln-356 residues. Upon phosphorylation by RIPK3, undergoes an active conformation (By similarity).

The coiled coil region 2 is responsible for homotrimerization.

Belongs to the protein kinase superfamily.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

References

1). Polystyrene nanoplastics and cadmium co-exposure aggravated cardiomyocyte damage in mice by regulating PANoptosis pathway. Environmental pollution (Barking, Essex : 1987), 2024 (PubMed: 38462200) [IF=8.9]

2). Polysaccharide from Strongylocentrotus nudus eggs regulates intestinal epithelial autophagy through CD36/PI3K-Akt pathway to ameliorate inflammatory bowel disease. International Journal of Biological Macromolecules, 2023 (PubMed: 37327932) [IF=8.2]

3). Pterocephin A, a Novel Triterpenoid Saponin from Pterocephalus hookeri Induced Liver Injury by Activation of Necroptosis. PHYTOMEDICINE, 2021 (PubMed: 33831690) [IF=7.9]

4). An integrated network pharmacology approach reveals that Ampelopsis grossedentata improves alcoholic liver disease via TLR4/NF-κB/MLKL pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38981149) [IF=7.9]

5). Histone deacetylase-mediated silencing of PSTPIP2 expression contributes to aristolochic acid nephropathy-induced PANoptosis. British journal of pharmacology, 2024 (PubMed: 38073114) [IF=7.3]

6). Protective Effect of Calpain Inhibition During Cold Ischemia on Ischemia–reperfusion Injury After Lung Transplantation. Transplantation, 2023 (PubMed: 36648297) [IF=6.2]

7). Cetylpyridinium chloride triggers paraptosis to suppress pancreatic tumor growth via the ERN1-MAP3K5-p38 pathway. iScience, 2024 (PubMed: 39211547) [IF=5.8]

8). Paeoniflorin directly binds to TNFR1 to regulate podocyte necroptosis in diabetic kidney disease. Frontiers in pharmacology, 2022 (PubMed: 36147345) [IF=5.6]

9). Inhibition of extracellular signal-regulated kinase/calpain-2 pathway reduces neuroinflammation and necroptosis after cerebral ischemia-reperfusion injury in a rat model of cardiac arrest. International Immunopharmacology, 2021 (PubMed: 33517223) [IF=5.6]

10). Cisatracurium besylate rescues Mycobacterium Tuberculosis-infected macrophages from necroptosis and enhances the bactericidal effect of isoniazid. International Immunopharmacology, 2023 (PubMed: 37182451) [IF=5.6]

Restrictive clause

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For Research Use Only.
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Phospho-MLKL (Ser358) Antibody - #AF7420