Phospho-BCL9L (Ser21 ) Antibody - #AF7415

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Product: Phospho-BCL9L (Ser21 ) Antibody
Catalog: AF7415
Description: Rabbit polyclonal antibody to Phospho-BCL9L (Ser21 )
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 157kDa; 157kD(Calculated).
Uniprot: Q86UU0
RRID: AB_2843855

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-BCL9L (Ser21 ) Antibody detects endogenous levels of BCL9L only when phosphorylated at Ser21 .
RRID:
AB_2843855
Cite Format: Affinity Biosciences Cat# AF7415, RRID:AB_2843855.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B-cell CLL; B-cell CLL/lymphoma 9-like protein; B-cell lymphoma 9-like protein; B9L; BCL9-2; BCL9-like protein; BCL9-related beta-catenin-binding protein; Bcl9l; BCL9L_HUMAN; DLNB11; Nuclear co factor of beta catenin signalling; Protein BCL9-2;

Immunogens

Immunogen:
Uniprot:

Q86UU0(BCL9L_HUMAN) >>Visit HPA database.

Gene(ID):
BCL9L(283149)
Expression:
Q86UU0 BCL9L_HUMAN:

Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.

Sequence:
MRILANKTRLPHPRRREAPGSPPLSPRGHCPPAPAKPMHPENKLTNHGKTGNGGAQSQHQNVNQGPTCNVGSKGVGAGNHGAKANQISPSNSSLKNPQAGVPPFSSLKGKVKRDRSVSVDSGEQREAGTPSLDSEAKEVAPRSKRRCVLERKQPYSGDEWCSGPDSEEDDKPIGATHNCNVADPAMAAPQLGPGQTTQLPLSESSVPGAPHGPPPGLRPDAPGGGGGGGGVPGKPPSQFVYVFTTHLANTAAEAVLQGRADSILAYHQQNVPRAKLDQAPKVPPTPEPLPLSTPSAGTPQSQPPPLPPPPPPAPGSAPPALPPEGPPEDSSQDLAPNSVGAASTGGGTGGTHPNTPTATTANNPLPPGGDPSSAPGPALLGEAAAPGNGQRSLVGSEGLSKEQLEHRERSLQTLRDIERLLLRSGETEPFLKGPPGGAGEGGPPAQAPPPPQQPPTAPPSGLKKYEEPLQSMISQTQSLGGPPLEHEVPGHPPGGDMGQQMNMMIQRLGQDSLTPEQVAWRKLQEEYYEEKRRKEEQIGLHGSRPLQDMMGMGGMMVRGPPPPYHSKPGDQWPPGMGAQLRGPMDVQDPMQLRGGPPFPGPRFPGNQIQRVPGFGGMQSMPMEVPMNAMQRPVRPGMGWTEDLPPMGGPSNFAQNTMPYPGGQGEAERFMTPRVREELLRHQLLEKRSMGMQRPLGMAGSGMGQSMEMERMMQAHRQMDPAMFPGQMAGGEGLAGTPMGMEFGGGRGLLSPPMGQSGLREVDPPMGPGNLNMNMNVNMNMNMNLNVQMTPQQQMLMSQKMRGPGDLMGPQGLSPEEMARVRAQNSSGVMGGPQKMLMPSQFPNQGQQGFSGGQGPYQAMSQDMGNTQDMFSPDQSSMPMSNVGTTRLSHMPLPPASNPPGTVHSAPNRGLGRRPSDLTISINQMGSPGMGHLKSPTLSQVHSPLVTSPSANLKSPQTPSQMVPLPSANPPGPLKSPQVLGSSLSVRSPTGSPSRLKSPSMAVPSPGWVASPKTAMPSPGVSQNKQPPLNMNSSTTLSNMEQGTLPPSGPRSSSSAPPANPPSGLMNPSLPFTSSPDPTPSQNPLSLMMTQMSKYAMPSSTPLYHNAIKTIATSDDELLPDRPLLPPPPPPQGSGPGISNSQPSQMHLNSAAAQSPMGMNLPGQQPLSHEPPPAMLPSPTPLGSNIPLHPNAQGTGGPPQNSMMMAPGGPDSLNAPCGPVPSSSQMMPFPPRLQQPHGAMAPTGGGGGGPGLQQHYPSGMALPPEDLPNQPPGPMPPQQHLMGKAMAGRMGDAYPPGVLPGVASVLNDPELSEVIRPTPTGIPEFDLSRIIPSEKPSSTLQYFPKSENQPPKAQPPNLHLMNLQNMMAEQTPSRPPNLPGQQGVQRGLNMSMCHPGQMSLLGRTGVPPQQGMVPHGLHQGVMSPPQGLMTQQNFMLMKQRGVGGEVYSQPPHMLSPQGSLMGPPPQQNLMVSHPLRQRSVSLDSQMGYLPAPGGMANLPF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Zebrafish
83
Xenopus
63
Chicken
56
Sheep
50
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86UU0 As Substrate

Site PTM Type Enzyme
S21 Phosphorylation
S25 Phosphorylation
K36 Acetylation
K43 Acetylation
K49 Acetylation
C68 S-Nitrosylation
K73 Acetylation
K83 Acetylation
S88 Phosphorylation
S93 Phosphorylation
S105 Phosphorylation
S106 Phosphorylation
K108 Acetylation
K110 Acetylation
S116 Phosphorylation
S118 Phosphorylation
S121 Phosphorylation
T129 Phosphorylation
K137 Acetylation
K275 Ubiquitination
K401 Ubiquitination
S410 Phosphorylation
S424 Phosphorylation
Y465 Phosphorylation
T514 Phosphorylation
K522 Sumoylation
Y527 Phosphorylation
K531 Ubiquitination
K534 Acetylation
R593 Methylation
R602 Methylation
R610 Methylation
R668 Methylation
R675 Methylation
R680 Methylation
S750 Phosphorylation
S813 Phosphorylation
R821 Methylation
S915 Phosphorylation
T918 Phosphorylation
S926 Phosphorylation
S934 Phosphorylation
T936 Phosphorylation
S938 Phosphorylation
S942 Phosphorylation
T946 Phosphorylation
S947 Phosphorylation
S949 Phosphorylation
S954 Phosphorylation
T957 Phosphorylation
S959 Phosphorylation
S975 Phosphorylation
S987 Phosphorylation
T989 Phosphorylation
S991 Phosphorylation
S993 Phosphorylation
S997 Phosphorylation
S999 Phosphorylation
S1004 Phosphorylation
S1010 Phosphorylation
T1013 Phosphorylation
S1017 Phosphorylation
S1021 Phosphorylation
K1334 Acetylation
K1344 Sumoylation
R1439 Methylation
Y1446 Phosphorylation
S1447 Phosphorylation
S1454 Phosphorylation
S1458 Phosphorylation
S1478 Phosphorylation
S1480 Phosphorylation

Research Backgrounds

Function:

Transcriptional regulator that acts as an activator. Promotes beta-catenin transcriptional activity. Plays a role in tumorigenesis. Enhances the neoplastic transforming activity of CTNNB1 (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in breast, ductal and invasive ductal carcinomas of the breast, sporadic colorectal adenomas and carcinomas (at protein level). Expressed in fetal brain. Expressed in lung, amygdala, eye, prostate, pancreatic and prostate cancers, head and neck tumors and embryonal tumor.

Subunit Structure:

Found in a complex with CDC73; CTNNB1 and PYGO1. Interacts with CTNNB1.

Family&Domains:

Tne C-terminal domain is important for its transactivation activity.

Belongs to the BCL9 family.

Restrictive clause

 

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