Phospho-Rictor (Ser1591) Antibody - #AF7411

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Product: Phospho-Rictor (Ser1591) Antibody
Catalog: AF7411
Description: Rabbit polyclonal antibody to Phospho-Rictor (Ser1591)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 192kDa; 192kD(Calculated).
Uniprot: Q6R327
RRID: AB_2843851

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 100ul $350 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
Phospho-Rictor (Ser1591) Antibody detects endogenous levels of Rictor only when phosphorylated at Ser1591.
RRID:
AB_2843851
Cite Format: Affinity Biosciences Cat# AF7411, RRID:AB_2843851.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AVO3; AVO3 homolog; DKFZp686B11164; hAVO3; KIAA1999; Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae); mAVO3; MGC39830; PIA; Pianissimo; Rapamycin insensitive companion of mTOR; Rapamycin-insensitive companion of mTOR; Rictor; RICTR; RICTR_HUMAN; RPTOR independent companion of MTOR complex 2; TORC2 specific protein AVO3;

Immunogens

Immunogen:
Uniprot:

Q6R327(RICTR_HUMAN) >>Visit HPA database.

Gene(ID):
RICTOR(253260)
Sequence:
MAAIGRGRSLKNLRVRGRNDSGEENVPLDLTREPSDNLREILQNVARLQGVSNMRKLGHLNNFTKLLCDIGHSEEKLGFHYEDIIICLRLALLNEAKEVRAAGLRALRYLIQDSSILQKVLKLKVDYLIARCIDIQQSNEVERTQALRLVRKMITVNASLFPSSVTNSLIAVGNDGLQERDRMVRACIAIICELALQNPEVVALRGGLNTILKNVIDCQLSRINEALITTILHLLNHPKTRQYVRADVELERILAPYTDFHYRHSPDTAEGQLKEDREARFLASKMGIIATFRSWAGIINLCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTEEFIEALLSVDPGRFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLLEGLVEVITNSDDHISVRATILLGELLHMANTILPHSHSHHLHCLPTLMNMAASFDIPKEKRLRASAALNCLKRFHEMKKRGPKPYSLHLDHIIQKAIATHQKRDQYLRVQKDIFILKDTEEALLINLRDSQVLQHKENLEWNWNLIGTILKWPNVNLRNYKDEQLHRFVRRLLYFYKPSSKLYANLDLDFAKAKQLTVVGCQFTEFLLESEEDGQGYLEDLVKDIVQWLNASSGMKPERSLQNNGLLTTLSQHYFLFIGTLSCHPHGVKMLEKCSVFQCLLNLCSLKNQDHLLKLTVSSLDYSRDGLARVILSKILTAATDACRLYATKHLRVLLRANVEFFNNWGIELLVTQLHDKNKTISSEALDILDEACEDKANLHALIQMKPALSHLGDKGLLLLLRFLSIPKGFSYLNERGYVAKQLEKWHREYNSKYVDLIEEQLNEALTTYRKPVDGDNYVRRSNQRLQRPHVYLPIHLYGQLVHHKTGCHLLEVQNIITELCRNVRTPDLDKWEEIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLAKQCEVLSIRGTCVYVLGLIAKTKQGCDILKCHNWDAVRHSRKHLWPVVPDDVEQLCNELSSIPSTLSLNSESTSSRHNSESESVPSSMFILEDDRFGSSSTSTFFLDINEDTEPTFYDRSGPIKDKNSFPFFASSKLVKNRILNSLTLPNKKHRSSSDPKGGKLSSESKTSNRRIRTLTEPSVDFNHSDDFTPISTVQKTLQLETSFMGNKHIEDTGSTPSIGENDLKFTKNFGTENHRENTSRERLVVESSTSSHMKIRSQSFNTDTTTSGISSMSSSPSRETVGVDATTMDTDCGSMSTVVSTKTIKTSHYLTPQSNHLSLSKSNSVSLVPPGSSHTLPRRAQSLKAPSIATIKSLADCNFSYTSSRDAFGYATLKRLQQQRMHPSLSHSEALASPAKDVLFTDTITMKANSFESRLTPSRFMKALSYASLDKEDLLSPINQNTLQRSSSVRSMVSSATYGGSDDYIGLALPVDINDIFQVKDIPYFQTKNIPPHDDRGARAFAHDAGGLPSGTGGLVKNSFHLLRQQMSLTEIMNSIHSDASLFLESTEDTGLQEHTDDNCLYCVCIEILGFQPSNQLSAICSHSDFQDIPYSDWCEQTIHNPLEVVPSKFSGISGCSDGVSQEGSASSTKSTELLLGVKTIPDDTPMCRILLRKEVLRLVINLSSSVSTKCHETGLLTIKEKYPQTFDDICLYSEVSHLLSHCTFRLPCRRFIQELFQDVQFLQMHEEAEAVLATPPKQPIVDTSAES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6R327 As Substrate

Site PTM Type Enzyme
R6 Methylation
S21 Phosphorylation
S35 Phosphorylation
K56 Ubiquitination
K65 Ubiquitination
K97 Ubiquitination
Y109 Phosphorylation
K213 Acetylation
Y243 Phosphorylation
S265 Phosphorylation
K274 Ubiquitination
S284 Phosphorylation
K374 Ubiquitination
S383 Phosphorylation
T424 Phosphorylation
K477 Ubiquitination
K507 Ubiquitination
K516 Ubiquitination
K522 Ubiquitination
K566 Ubiquitination
K582 Acetylation
K719 Ubiquitination
T733 Phosphorylation
K764 Ubiquitination
K781 Ubiquitination
K791 Ubiquitination
K813 Ubiquitination
Y823 Phosphorylation
K826 Ubiquitination
K830 Ubiquitination
Y835 Phosphorylation
S837 Phosphorylation
K856 Ubiquitination
Y863 Phosphorylation
K916 Ubiquitination
K921 Ubiquitination
K979 Ubiquitination
K986 Methylation
K986 Ubiquitination
S1026 Phosphorylation
S1028 Phosphorylation
S1030 Phosphorylation
S1031 Phosphorylation
S1035 Phosphorylation
S1037 Phosphorylation
Y1073 Phosphorylation
K1082 Ubiquitination
S1084 Phosphorylation
S1090 Phosphorylation
K1092 Ubiquitination
S1101 Phosphorylation
T1103 Phosphorylation
K1107 Ubiquitination
K1116 Acetylation
K1119 Acetylation
K1125 Acetylation
K1125 Ubiquitination
T1133 Phosphorylation
T1135 Phosphorylation Q9UBS0 (RPS6KB2) , P31749 (AKT1) , O00141 (SGK1) , P23443 (RPS6KB1)
S1138 Phosphorylation
S1144 Phosphorylation
T1148 Phosphorylation
T1161 Phosphorylation
S1162 Phosphorylation
K1167 Ubiquitination
T1172 Phosphorylation
S1174 Phosphorylation
T1175 Phosphorylation
S1177 Phosphorylation
K1184 Ubiquitination
K1187 Ubiquitination
S1211 Phosphorylation
S1217 Phosphorylation
S1219 Phosphorylation
T1222 Phosphorylation
T1224 Phosphorylation
T1225 Phosphorylation
T1226 Phosphorylation
S1227 Phosphorylation
S1230 Phosphorylation
S1231 Phosphorylation
S1234 Phosphorylation
S1235 Phosphorylation P49841 (GSK3B)
K1265 Ubiquitination
S1267 Phosphorylation
Y1269 Phosphorylation
T1271 Phosphorylation
S1274 Phosphorylation
S1278 Phosphorylation
K1281 Ubiquitination
S1282 Phosphorylation
S1284 Phosphorylation
S1286 Phosphorylation
S1292 Phosphorylation
S1293 Phosphorylation
T1295 Phosphorylation
S1302 Phosphorylation
K1304 Methylation
K1304 Ubiquitination
S1307 Phosphorylation
T1310 Phosphorylation
K1312 Acetylation
K1312 Methylation
K1312 Ubiquitination
S1313 Phosphorylation
S1320 Phosphorylation
Y1321 Phosphorylation
Y1330 Phosphorylation
T1332 Phosphorylation
K1334 Methylation
K1334 Ubiquitination
S1344 Phosphorylation
S1346 Phosphorylation
S1348 Phosphorylation
S1353 Phosphorylation
T1361 Phosphorylation
K1367 Methylation
S1370 Phosphorylation
S1373 Phosphorylation
T1376 Phosphorylation
K1382 Ubiquitination
S1385 Phosphorylation
Y1386 Phosphorylation
S1388 Phosphorylation
K1391 Ubiquitination
S1396 Phosphorylation
T1402 Phosphorylation
S1406 Phosphorylation
S1407 Phosphorylation
S1411 Phosphorylation
S1414 Phosphorylation
S1415 Phosphorylation
T1417 Phosphorylation
Y1418 Phosphorylation
S1421 Phosphorylation
Y1444 Phosphorylation
T1447 Phosphorylation
K1448 Ubiquitination
S1470 Phosphorylation
T1472 Phosphorylation
K1477 Ubiquitination
S1479 Phosphorylation
S1571 Phosphorylation
S1574 Phosphorylation
S1577 Phosphorylation
S1581 Phosphorylation
S1585 Phosphorylation
K1590 Ubiquitination
S1591 Phosphorylation
T1592 Phosphorylation
K1599 Ubiquitination
T1605 Phosphorylation
K1640 Ubiquitination
T1695 Phosphorylation P49841 (GSK3B) , P49840 (GSK3A)

Research Backgrounds

Function:

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.

PTMs:

Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.

Subunit Structure:

Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to MTOR and PRR5 within the TORC2 complex. Interacts with CCDC28B. Interacts with NBN. Interacts with PRR5L. Interacts with SIK3.

(Microbial infection) Interacts with vaccinia virus protein F17; this interaction dysregulates mTOR.

Family&Domains:

Belongs to the RICTOR family.

Research Fields

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

References

1). Maternal hyperglycemia inhibits pulmonary vasculogenesis during mouse fetal lung development by promoting GβL Ubiquitination-dependent mammalian target of Rapamycin assembly. Diabetology & Metabolic Syndrome, 2023 (PubMed: 36927703) [IF=4.8]

Application: WB    Species: Mouse    Sample: lung tissues

Fig. 2 Upregulation of mTORC1 and downregulation of mTORC2 in the GDM fetal lungs. A The protein expression of TRAF2, OTUD7B, p-mTOR, mTOR, and GβL in the control and GDM fetal lung tissues. B The determined protein levels of p-Sin1, Sin1, p-Rictor, Rictor, p-Raptor, Raptor, p-Akt, and Akt in the control and GDM fetal lung tissues. C The relative protein levels of TRAF2, OTUD7B, p-mTOR, mTOR, and GL in the control and GDM fetal lung tissues. D The relative protein levels of p-Sin1, Sin1, p-Rictor, Rictor, p-Raptor, Raptor, p-Akt, and Akt in the control and GDM fetal lung tissues (n = 3 in triplicate, **p 

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