Phospho-TBC1D1 (Ser237) Antibody - #AF7409
| Product: | Phospho-TBC1D1 (Ser237) Antibody |
| Catalog: | AF7409 |
| Description: | Rabbit polyclonal antibody to Phospho-TBC1D1 (Ser237) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat, Monkey |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 130~160kDa; 133kD(Calculated). |
| Uniprot: | Q86TI0 |
| RRID: | AB_2843849 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF7409, RRID:AB_2843849.
Fold/Unfold
KIAA1108; TBC 1; TBC; TBC1 (tre 2/USP6, BUB2, cdc16) domain family, member 1; TBC1; TBC1 domain family member 1; Tbc1d1; TBCD1_HUMAN;
Immunogens
- Q86TI0 TBCD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPITFTARKHLLSNEVSVDFGLQLVGSLPVHSLTTMPMLPWVVAEVRRLSRQSTRKEPVTKQVRLCVSPSGLRCEPEPGRSQQWDPLIYSSIFECKPQRVHKLIHNSHDPSYFACLIKEDAVHRQSICYVFKADDQTKVPEIISSIRQAGKIARQEELHCPSEFDDTFSKKFEVLFCGRVTVAHKKAPPALIDECIEKFNHVSGSRGSESPRPNPPHAAPTGSQEPVRRPMRKSFSQPGLRSLAFRKELQDGGLRSSGFFSSFEESDIENHLISGHNIVQPTDIEENRTMLFTIGQSEVYLISPDTKKIALEKNFKEISFCSQGIRHVDHFGFICRESSGGGGFHFVCYVFQCTNEALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGCPLQSLHKLCERIEGMNSSKTKLELQKHLTTLTNQEQATIFEEVQKLRPRNEQRENELIISFLRCLYEEKQKEHIHIGEMKQTSQMAAENIGSELPPSATRFRLDMLKNKAKRSLTESLESILSRGNKARGLQEHSISVDLDSSLSSTLSNTSKEPSVCEKEALPISESSFKLLGSSEDLSSDSESHLPEEPAPLSPQQAFRRRANTLSHFPIECQEPPQPARGSPGVSQRKLMRYHSVSTETPHERKDFESKANHLGDSGGTPVKTRRHSWRQQIFLRVATPQKACDSSSRYEDYSELGELPPRSPLEPVCEDGPFGPPPEEKKRTSRELRELWQKAILQQILLLRMEKENQKLQASENDLLNKRLKLDYEEITPCLKEVTTVWEKMLSTPGRSKIKFDMEKMHSAVGQGVPRHHRGEIWKFLAEQFHLKHQFPSKQQPKDVPYKELLKQLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGILLLHMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMIFLQGTEVIFKVALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEKTINQVFEMDIAKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLDLLEQLQVANGRIQSLEATIEKLLSSESKLKQAMLTLELERSALLQTVEELRRRSAEPSDREPECTQPEPTGD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q86TI0 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S69 | Phosphorylation | Uniprot | |
| Y90 | Phosphorylation | Uniprot | |
| Y113 | Phosphorylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S237 | Phosphorylation | Q13131 (PRKAA1) , P54646 (PRKAA2) | Uniprot |
| K248 | Ubiquitination | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| T410 | Phosphorylation | Uniprot | |
| K497 | Acetylation | Uniprot | |
| K501 | Acetylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| T505 | Phosphorylation | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| S513 | Phosphorylation | Uniprot | |
| S527 | Phosphorylation | Uniprot | |
| S559 | Phosphorylation | Uniprot | |
| S565 | Phosphorylation | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| S573 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| S585 | Phosphorylation | Uniprot | |
| T596 | Phosphorylation | P31749 (AKT1) , Q13131 (PRKAA1) | Uniprot |
| S598 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| S627 | Phosphorylation | Uniprot | |
| S649 | Phosphorylation | Uniprot | |
| T652 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| T671 | Phosphorylation | Uniprot | |
| S695 | Phosphorylation | Uniprot | |
| K743 | Ubiquitination | Uniprot | |
| K754 | Ubiquitination | Uniprot | |
| K768 | Ubiquitination | Uniprot | |
| K826 | Ubiquitination | Uniprot | |
| K835 | Ubiquitination | Uniprot | |
| Y880 | Phosphorylation | Uniprot | |
| K1092 | Ubiquitination | Uniprot | |
| S1137 | Phosphorylation | Uniprot |
Research Backgrounds
May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues. Involved in the trafficking and translocation of GLUT4-containing vesicles and insulin-stimulated glucose uptake into cells (By similarity).
Insulin-stimulated phosphorylation by AKT family kinases stimulates SLC2A4/GLUT4 translocation.
Nucleus.
Interacts with APPL2 (via BAR domain); interaction is dependent of TBC1D1 phosphorylation at Ser-235; interaction diminishes the phosphorylation of TBC1D1 at Thr-596, resulting in inhibition of SLC2A4/GLUT4 translocation and glucose uptake.
Research Fields
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.