Phospho-MAPK6/ERK3 (Ser189) Antibody - #AF7407
| Product: | Phospho-MAPK6/ERK3 (Ser189) Antibody |
| Catalog: | AF7407 |
| Description: | Rabbit polyclonal antibody to Phospho-MAPK6/ERK3 (Ser189) |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 85kDa; 83kD(Calculated). |
| Uniprot: | Q16659 |
| RRID: | AB_2843847 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF7407, RRID:AB_2843847.
Fold/Unfold
ERK-3; ERK3; Extracellular signal regulated kinase 3; Extracellular signal regulated kinase p97; Extracellular signal-regulated kinase 3; HsT17250; MAP kinase 6; MAP kinase isoform p97; MAPK 6; MAPK6; Mitogen activated protein kinase 6; Mitogen-activated protein kinase 6; MK06_HUMAN; p97 MAPK; p97-MAPK; PRKM6; Protein kinase mitogen activated 5; Protein kinase mitogen activated 6;
Immunogens
Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
- Q16659 MK06_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEKFESLMNIHGFDLGSRYMDLKPLGCGGNGLVFSAVDNDCDKRVAIKKIVLTDPQSVKHALREIKIIRRLDHDNIVKVFEILGPSGSQLTDDVGSLTELNSVYIVQEYMETDLANVLEQGPLLEEHARLFMYQLLRGLKYIHSANVLHRDLKPANLFINTEDLVLKIGDFGLARIMDPHYSHKGHLSEGLVTKWYRSPRLLLSPNNYTKAIDMWAAGCIFAEMLTGKTLFAGAHELEQMQLILESIPVVHEEDRQELLSVIPVYIRNDMTEPHKPLTQLLPGISREALDFLEQILTFSPMDRLTAEEALSHPYMSIYSFPMDEPISSHPFHIEDEVDDILLMDETHSHIYNWERYHDCQFSEHDWPVHNNFDIDEVQLDPRALSDVTDEEEVQVDPRKYLDGDREKYLEDPAFDTNYSTEPCWQYSDHHENKYCDLECSHTCNYKTRSSSYLDNLVWRESEVNHYYEPKLIIDLSNWKEQSKEKSDKKGKSKCERNGLVKAQIALEEASQQLAGKEREKNQGFDFDSFIAGTIQLSSQHEPTDVVDKLNDLNSSVSQLELKSLISKSVSQEKQEKGMANLAQLEALYQSSWDSQFVSGGEDCFFINQFCEVRKDEQVEKENTYTSYLDKFFSRKEDTEMLETEPVEDGKLGERGHEEGFLNNSGEFLFNKQLESIGIPQFHSPVGSPLKSIQATLTPSAMKSSPQIPHQTYSSILKHLN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q16659 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K4 | Ubiquitination | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| Y182 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Q16659 (MAPK6) , Q13177 (PAK2) , O75914 (PAK3) | Uniprot |
| K195 | Ubiquitination | Uniprot | |
| Y209 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| Y357 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| T389 | Phosphorylation | Uniprot | |
| K408 | Ubiquitination | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| Y453 | Phosphorylation | Uniprot | |
| Y467 | Phosphorylation | Uniprot | |
| Y468 | Phosphorylation | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| K502 | Ubiquitination | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| S558 | Phosphorylation | Uniprot | |
| K563 | Ubiquitination | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| K621 | Ubiquitination | Uniprot | |
| K631 | Ubiquitination | Uniprot | |
| K636 | Ubiquitination | Uniprot | |
| T644 | Phosphorylation | Uniprot | |
| K651 | Ubiquitination | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| K672 | Ubiquitination | Uniprot | |
| S684 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S688 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K691 | Ubiquitination | Uniprot | |
| T698 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K703 | Ubiquitination | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| S705 | Phosphorylation | P06493 (CDK1) | Uniprot |
PTMs - Q16659 As Enzyme
Research Backgrounds
Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).
Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.
Ubiquitination at Met-1 leads to degradation by the proteasome pathway.
Cytoplasm. Nucleus.
Note: Translocates to the cytoplasm following interaction with MAPKAPK5.
Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
Heterodimer with ERK4/MAPK4. Interacts with (via FRIEDE motif) MAPKAPK5 (By similarity). Interacts with UBE3A; this interaction may be indirect and mediated by HERC2, possibly via HERC2 interaction with NEURL4.
In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Research Fields
· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)
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