Phospho-TrkC (Tyr705) Antibody | Affinity Biosciences

WB
IHC
pElisa

1/5

Western blot analysis of extracts from various samples, using Phospho-Trk C (Tyr705) Antibody.

Western blot analysis of extracts from LPS treated RAW264.

Western blot analysis of extracts from various samples, using Phospho-TrkC (Tyr705) Antibody.

AF7405 at 1/100 staining rat lung tissue sections by IHC-P.

peptide-ELISA analysis of AF7405.

Product:	Phospho-TrkC (Tyr705) Antibody
Catalog:	AF7405
Description:	Rabbit polyclonal antibody to Phospho-TrkC (Tyr705)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Bovine, Horse, Chicken
Mol.Wt.:	94kDa; 94kD(Calculated).
Uniprot:	Q16288
RRID:	AB_2843845

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

Phospho-TrkC (Tyr705) Antibody detects endogenous levels of TrkC only when phosphorylated at Tyr705.

RRID:

AB_2843845
Cite Format: Affinity Biosciences Cat# AF7405, RRID:AB_2843845.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EC 2.7.10.1; ETS related protein neurotrophic receptor tyrosine kinase fusion; ETS related protein neurotrophic receptor tyrosine kinase fusion protein; ETV6 NTRK3 fusion; GP145 TrkC; gp145(trkC); GP145-TrkC; GP145TrkC; Neurotrophic tyrosine kinase receptor type 3; Neurotrophin 3 receptor; NT 3 growth factor receptor; NT 3 growth factor receptor precursor; NT 3 receptor; NT-3 growth factor receptor; Ntrk3; NTRK3_HUMAN; OTTHUMP00000192915; TRK C; Trk-C; TRKC; TrkC tyrosine kinase; Tyrosine kinase receptor C;

Immunogens

Immunogen:

Uniprot:

Q16288(NTRK3_HUMAN) >>Visit HPA database.

Gene(ID):

NTRK3(4916)

Expression:

Q16288 NTRK3_HUMAN:

Widely expressed but mainly in nervous tissue. Isoform 2 is expressed at higher levels in adult brain than in fetal brain.

Sequence:

Q16288 NTRK3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDVSLCPAKCSFWRIFLLGSVWLDYVGSVLACPANCVCSKTEINCRRPDDGNLFPLLEGQDSGNSNGNASINITDISRNITSIHIENWRSLHTLNAVDMELYTGLQKLTIKNSGLRSIQPRAFAKNPHLRYINLSSNRLTTLSWQLFQTLSLRELQLEQNFFNCSCDIRWMQLWQEQGEAKLNSQNLYCINADGSQLPLFRMNISQCDLPEISVSHVNLTVREGDNAVITCNGSGSPLPDVDWIVTGLQSINTHQTNLNWTNVHAINLTLVNVTSEDNGFTLTCIAENVVGMSNASVALTVYYPPRVVSLEEPELRLEHCIEFVVRGNPPPTLHWLHNGQPLRESKIIHVEYYQEGEISEGCLLFNKPTHYNNGNYTLIAKNPLGTANQTINGHFLKEPFPESTDNFILFDEVSPTPPITVTHKPEEDTFGVSIAVGLAAFACVLLVVLFVMINKYGRRSKFGMKGPVAVISGEEDSASPLHHINHGITTPSSLDAGPDTVVIGMTRIPVIENPQYFRQGHNCHKPDTYVQHIKRRDIVLKRELGEGAFGKVFLAECYNLSPTKDKMLVAVKALKDPTLAARKDFQREAELLTNLQHEHIVKFYGVCGDGDPLIMVFEYMKHGDLNKFLRAHGPDAMILVDGQPRQAKGELGLSQMLHIASQIASGMVYLASQHFVHRDLATRNCLVGANLLVKIGDFGMSRDVYSTDYYRLFNPSGNDFCIWCEVGGHTMLPIRWMPPESIMYRKFTTESDVWSFGVILWEIFTYGKQPWFQLSNTEVIECITQGRVLERPRVCPKEVYDVMLGCWQREPQQRLNIKEIYKILHALGKATPIYLDILG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Chicken

100

Sheep

Dog

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16288 As Substrate

Q16288

Site	PTM Type	Enzyme	Source
Y131	Phosphorylation		Uniprot
T220	Phosphorylation		Uniprot
T386	Phosphorylation		Uniprot
Y516	Phosphorylation	Q16288 (NTRK3)	Uniprot
Y558	Phosphorylation		Uniprot
S701	Phosphorylation		Uniprot
Y705	Phosphorylation		Uniprot
Y709	Phosphorylation		Uniprot
Y710	Phosphorylation		Uniprot
Y800	Phosphorylation		Uniprot

PTMs - Q16288 As Enzyme

Q16288

Substrate	Site	Source
Q16288 (NTRK3)	Y516	Uniprot

Research Backgrounds

Q16288_NTRK3_HUMAN

Function:

Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation.

PTMs:

Ligand-mediated auto-phosphorylation.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Tissue Specificity:

Widely expressed but mainly in nervous tissue. Isoform 2 is expressed at higher levels in adult brain than in fetal brain.

Subunit Structure:

Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures (By similarity). Binds SH2B2. Interacts with SQSTM1 and KIDINS220 (By similarity). Interacts with PTPRS. Interacts with MAPK8IP3/JIP3 (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Research Fields

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

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Phospho-TrkC (Tyr705) Antibody - #AF7405