Product: Phospho-Zyxin (Tyr316) Antibody
Catalog: AF7402
Description: Rabbit polyclonal antibody to Phospho-Zyxin (Tyr316)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 80kDa; 61kD(Calculated).
Uniprot: Q15942
RRID: AB_2843842

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(90%), Horse(100%), Sheep(80%), Rabbit(90%), Dog(90%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
Phospho-Zyxin (Tyr316) Antibody detects endogenous levels of Zyxin only when phosphorylated at Tyr316.
RRID:
AB_2843842
Cite Format: Affinity Biosciences Cat# AF7402, RRID:AB_2843842.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ESP 2; ESP2; HED 2; HED2; ZYX; ZYX protein; Zyxin 2; Zyxin2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAAPRPSPAISVSVSAPAFYAPQKKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYKCEDCGKPLSIEADDNGCFPLDGHVLCRKCHTARAQT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
90
Dog
90
Rabbit
90
Sheep
80
Chicken
80
Xenopus
70
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15942 As Substrate

Site PTM Type Enzyme
A2 Acetylation
R5 Methylation
S7 Phosphorylation
S13 Phosphorylation
S15 Phosphorylation
Y20 Phosphorylation
K24 Acetylation
K25 Acetylation
K25 Methylation
S44 Phosphorylation
S116 Phosphorylation
S142 Phosphorylation P31749 (AKT1)
S143 Phosphorylation
S150 Phosphorylation
K165 Acetylation
K165 Methylation
K165 Ubiquitination
S169 Phosphorylation
S170 Phosphorylation
Y172 Phosphorylation
T179 Phosphorylation
K201 Methylation
S202 Phosphorylation
S206 Phosphorylation
T250 Phosphorylation
S258 Phosphorylation
S259 Phosphorylation
K265 Acetylation
K265 Ubiquitination
S267 Phosphorylation
T270 Phosphorylation
K272 Acetylation
K272 Methylation
K272 Ubiquitination
T274 Phosphorylation
S278 Phosphorylation
K279 Acetylation
K279 Methylation
K279 Ubiquitination
S281 Phosphorylation
S288 Phosphorylation
S290 Phosphorylation
K295 Ubiquitination
S303 Phosphorylation P24941 (CDK2)
T306 Phosphorylation P24941 (CDK2)
S308 Phosphorylation P24941 (CDK2)
S313 Phosphorylation
T315 Phosphorylation
Y316 Phosphorylation
K328 Ubiquitination
R343 Methylation
S344 Phosphorylation
T352 Phosphorylation
S505 Phosphorylation
K528 Acetylation

Research Backgrounds

Function:

Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Nucleus. Cell junction>Focal adhesion.
Note: Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES. Interacts with NEBL (isoform 2). Interacts with SYNPO2.

(Microbial infection) Interacts with human papillomavirus type 6/HPV6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18.

Family&Domains:

Belongs to the zyxin/ajuba family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

References

1). The protective effect of C-phycocyanin in male mouse reproductive system. Food & Function, 2022 (PubMed: 35167640) [IF=6.1]

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