Phospho-GRK1 (Ser21) Antibody | Affinity Biosciences

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Product:	Phospho-GRK1 (Ser21) Antibody
Catalog:	AF7399
Description:	Rabbit polyclonal antibody to Phospho-GRK1 (Ser21)
Application:	WB
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.:	60kDa; 64kD(Calculated).
Uniprot:	Q15835
RRID:	AB_2843839

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(100%), Bovine(88%), Horse(88%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-GRK1 (Ser21) Antibody detects endogenous levels of GRK1 only when phosphorylated at Ser21.

RRID:

AB_2843839
Cite Format: Affinity Biosciences Cat# AF7399, RRID:AB_2843839.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EC=2.7.11.14; G protein-coupled receptor kinase 1; GPRK1; Grk1; Rhodopsin kinase; RHOK; RK; RK_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q15835(GRK1_HUMAN) >>Visit HPA database.

Gene(ID):

GRK1(6011)

Expression:

Q15835 GRK1_HUMAN:

Retinal-specific. Expressed in rods and cones cells.

Sequence:

Q15835 GRK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDFGSLETVVANSAFIAARGSFDGSSSQPSRDKKYLAKLKLPPLSKCESLRDSLSLEFESVCLEQPIGKKLFQQFLQSAEKHLPALELWKDIEDYDTADNDLQPQKAQTILAQYLDPQAKLFCSFLDEGIVAKFKEGPVEIQDGLFQPLLQATLAHLGQAPFQEYLGSLYFLRFLQWKWLEAQPMGEDWFLDFRVLGKGGFGEVSACQMKATGKLYACKKLNKKRLKKRKGYQGAMVEKKILMKVHSRFIVSLAYAFETKADLCLVMTIMNGGDIRYHIYNVNEENPGFPEPRALFYTAQIICGLEHLHQRRIVYRDLKPENVLLDNDGNVRISDLGLAVELLDGQSKTKGYAGTPGFMAPELLQGEEYDFSVDYFALGVTLYEMIAARGPFRARGEKVENKELKHRIISEPVKYPDKFSQASKDFCEALLEKDPEKRLGFRDETCDKLRAHPLFKDLNWRQLEAGMLMPPFIPDSKTVYAKDIQDVGAFSTVKGVAFDKTDTEFFQEFATGNCPIPWQEEMIETGIFGELNVWRSDGQMPDDMKGISGGSSSSSKSGMCLVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

100

Xenopus

100

Rabbit

100

Horse

Bovine

Sheep

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15835 As Substrate

Q15835

Site	PTM Type	Enzyme	Source
S21	Phosphorylation	Q15835 (GRK1) , P17612 (PRKACA)	Uniprot
S78	Phosphorylation		Uniprot
K81	Acetylation		Uniprot
K418	Acetylation		Uniprot
K424	Acetylation		Uniprot
K437	Acetylation		Uniprot
S491	Phosphorylation	Q15835 (GRK1)	Uniprot
T492	Phosphorylation	Q15835 (GRK1)	Uniprot

PTMs - Q15835 As Enzyme

Q15835

Substrate	Site	Source
P08100 (RHO)	S334	Uniprot
P08100 (RHO)	S338	Uniprot
P08100 (RHO)	S343	Uniprot
P41145 (OPRK1)	S358	Uniprot
Q15835 (GRK1)	S21	Uniprot
Q15835 (GRK1)	S491	Uniprot
Q15835 (GRK1)	T492	Uniprot
Q5NUL3-2 (FFAR4)	T347	Uniprot
Q5NUL3-2 (FFAR4)	S350	Uniprot
Q5NUL3-2 (FFAR4)	S357	Uniprot

Research Backgrounds

Q15835_GRK1_HUMAN

Function:

Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity). May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).

PTMs:

Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.

Farnesylation is required for full activity.

Subcellular Location:

Membrane>Lipid-anchor. Cell projection>Cilium>Photoreceptor outer segment.
Note: Subcellular location is not affected by light or dark conditions.

Tissue Specificity:

Retinal-specific. Expressed in rods and cones cells.

Subunit Structure:

Interacts (via N-terminus) with RCVRN (via C-terminus); the interaction is Ca(2+)-dependent (By similarity). Interacts (when prenylated) with PDE6D; this promotes release from membranes (By similarity). May form a complex composed of RHO, GRK1 and RCVRN in a Ca(2+)-dependent manner; RCVRN prevents the interaction between GRK1 and RHO (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

· Organismal Systems > Sensory system > Phototransduction.

Restrictive clause

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For Research Use Only.
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Phospho-GRK1 (Ser21) Antibody - #AF7399