Phospho-NCoA2 (Ser698/Ser699) Antibody - #AF7394

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Product: Phospho-NCoA2 (Ser698/Ser699) Antibody
Catalog: AF7394
Description: Rabbit polyclonal antibody to Phospho-NCoA2 (Ser698/Ser699)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 175kDa; 159kD(Calculated).
Uniprot: Q15596
RRID: AB_2843834

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-NCoA2 (Ser698/Ser699) Antibody detects endogenous levels of NCoA2 only when phosphorylated at Ser698/699.
RRID:
AB_2843834
Cite Format: Affinity Biosciences Cat# AF7394, RRID:AB_2843834.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bHLHe75; Class E basic helix-loop-helix protein 75; glucocorticoid receptor interacting protein 1; GRIP 1; GRIP1; hTIF2; MED1; MGC138808; NCoA 2; NCoA-2; Ncoa2; NCOA2_HUMAN; Nuclear receptor coactivator 2; Oncogene FGR; p160; SRC 2; src2; Steroid receptor coactivator 2; TIF 2; TIF2; Transcriptional intermediary factor 2;

Immunogens

Immunogen:
Uniprot:

Q15596(NCOA2_HUMAN) >>Visit HPA database.

Gene(ID):
NCOA2(10499)
Sequence:
MSGMGENTSDPSRAETRKRKECPDQLGPSPKRNTEKRNREQENKYIEELAELIFANFNDIDNFNFKPDKCAILKETVKQIRQIKEQEKAAAANIDEVQKSDVSSTGQGVIDKDALGPMMLEALDGFFFVVNLEGNVVFVSENVTQYLRYNQEELMNKSVYSILHVGDHTEFVKNLLPKSIVNGGSWSGEPPRRNSHTFNCRMLVKPLPDSEEEGHDNQEAHQKYETMQCFAVSQPKSIKEEGEDLQSCLICVARRVPMKERPVLPSSESFTTRQDLQGKITSLDTSTMRAAMKPGWEDLVRRCIQKFHAQHEGESVSYAKRHHHEVLRQGLAFSQIYRFSLSDGTLVAAQTKSKLIRSQTTNEPQLVISLHMLHREQNVCVMNPDLTGQTMGKPLNPISSNSPAHQALCSGNPGQDMTLSSNINFPINGPKEQMGMPMGRFGGSGGMNHVSGMQATTPQGSNYALKMNSPSQSSPGMNPGQPTSMLSPRHRMSPGVAGSPRIPPSQFSPAGSLHSPVGVCSSTGNSHSYTNSSLNALQALSEGHGVSLGSSLASPDLKMGNLQNSPVNMNPPPLSKMGSLDSKDCFGLYGEPSEGTTGQAESSCHPGEQKETNDPNLPPAVSSERADGQSRLHDSKGQTKLLQLLTTKSDQMEPSPLASSLSDTNKDSTGSLPGSGSTHGTSLKEKHKILHRLLQDSSSPVDLAKLTAEATGKDLSQESSSTAPGSEVTIKQEPVSPKKKENALLRYLLDKDDTKDIGLPEITPKLERLDSKTDPASNTKLIAMKTEKEEMSFEPGDQPGSELDNLEEILDDLQNSQLPQLFPDTRPGAPAGSVDKQAIINDLMQLTAENSPVTPVGAQKTALRISQSTFNNPRPGQLGRLLPNQNLPLDITLQSPTGAGPFPPIRNSSPYSVIPQPGMMGNQGMIGNQGNLGNSSTGMIGNSASRPTMPSGEWAPQSSAVRVTCAATTSAMNRPVQGGMIRNPAASIPMRPSSQPGQRQTLQSQVMNIGPSELEMNMGGPQYSQQQAPPNQTAPWPESILPIDQASFASQNRQPFGSSPDDLLCPHPAAESPSDEGALLDQLYLALRNFDGLEEIDRALGIPELVSQSQAVDPEQFSSQDSNIMLEQKAPVFPQQYASQAQMAQGSYSPMQDPNFHTMGQRPSYATLRMQPRPGLRPTGLVQNQPNQLRLQLQHRLQAQQNRQPLMNQISNVSNVNLTLRPGVPTQAPINAQMLAQRQREILNQHLRQRQMHQQQQVQQRTLMMRGQGLNMTPSMVAPSGMPATMSNPRIPQANAQQFPFPPNYGISQQPDPGFTGATTPQSPLMSPRMAHTQSPMMQQSQANPAYQAPSDINGWAQGNMGGNSMFSQQSPPHFGQQANTSMYSNNMNINVSMATNTGGMSSMNQMTGQISMTSVTSVPTSGLSSMGPEQVNDPALRGGNLFPNQLPGMDMIKQEGDTTRKYC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
91
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15596 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T8 Phosphorylation
S29 Phosphorylation
K31 Acetylation
T76 Phosphorylation
K78 Acetylation
K99 Ubiquitination
S161 Phosphorylation
S195 Phosphorylation
K205 Ubiquitination
S210 Phosphorylation
K239 Sumoylation
K279 Ubiquitination
K293 Ubiquitination
K306 Ubiquitination
K320 Ubiquitination
T456 Phosphorylation
T457 Phosphorylation
S469 Phosphorylation P68400 (CSNK2A1) , P50750 (CDK9)
S471 Phosphorylation
S474 Phosphorylation
S487 Phosphorylation P50750 (CDK9) , P68400 (CSNK2A1)
R489 Methylation
S493 Phosphorylation P50750 (CDK9) , P68400 (CSNK2A1)
S499 Phosphorylation P50750 (CDK9) , P68400 (CSNK2A1)
R501 Methylation
S554 Phosphorylation
S565 Phosphorylation
S579 Phosphorylation
K636 Acetylation
K640 Acetylation
K640 Sumoylation
K640 Ubiquitination
S655 Phosphorylation
S660 Phosphorylation
S668 Phosphorylation
T669 Phosphorylation
S671 Phosphorylation
S675 Phosphorylation
T681 Phosphorylation
S682 Phosphorylation
S697 Phosphorylation
S698 Phosphorylation
S699 Phosphorylation
K705 Methylation
K705 Ubiquitination
K713 Acetylation
S716 Phosphorylation
S719 Phosphorylation
S721 Phosphorylation
T722 Phosphorylation
S726 Phosphorylation
T729 Phosphorylation
K731 Sumoylation
S736 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1) , Q16539 (MAPK14)
K738 Acetylation
K739 Acetylation
Y747 Phosphorylation
K765 Sumoylation
S771 Phosphorylation
T773 Phosphorylation
K780 Acetylation
K785 Acetylation
K785 Sumoylation
K785 Ubiquitination
K788 Sumoylation
T847 Phosphorylation
S851 Phosphorylation
T854 Phosphorylation
R874 Methylation
K1129 Sumoylation
R1162 Methylation
R1169 Methylation
R1173 Methylation
R1177 Methylation
R1190 Methylation
R1196 Methylation
R1203 Methylation
R1221 Methylation
R1240 Methylation
R1248 Methylation
R1250 Methylation
R1261 Methylation
R1266 Methylation
K1454 Sumoylation

Research Backgrounds

Function:

Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts (via C-terminus) with CREBBP. Interacts with ESR1, HIF1A, NCOA1, APEX, NR3C1, NR3C2, CARM1, RARA, and RXRA. Present in a complex containing CARM1 and EP300/P300. Interacts with CASP8AP2 and TTLL5/STAMP. Interacts with PSMB9 and DDX5. Interacts (via LXXLL 1, 2 and 3 motifs) with RORA and RORC (via AF-2 motif). Interacts with RWDD3. Interacts with CLOCK and ARNTL/BMAL1 (By similarity). Interacts with NR4A3; potentiates the activity of the NR4A3 (By similarity). Interacts with NR1H3.

Family&Domains:

Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.

The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.

Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.

Belongs to the SRC/p160 nuclear receptor coactivator family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

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