Phospho-p40 phox (Tyr243) Antibody - #AF7392
| Product: | Phospho-p40 phox (Tyr243) Antibody |
| Catalog: | AF7392 |
| Description: | Rabbit polyclonal antibody to Phospho-p40 phox (Tyr243) |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Prediction: | Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 39kDa; 39kD(Calculated). |
| Uniprot: | Q15080 |
| RRID: | AB_2843832 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF7392, RRID:AB_2843832.
Fold/Unfold
CGD3; MGC3810; NCF 4; NCF; NCF-4; Ncf4; NCF4_HUMAN; Neutrophil cytosol factor 4; Neutrophil cytosolic factor 4; Neutrophil NADPH oxidase factor 4; p40-phox; p40phox; SH3 and PX domain-containing protein 4; SH3PXD4;
Immunogens
- Q15080 NCF4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVAQQLRAESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFHALQSKLEERFGPDSKSSALACTLPTLPAKVYVGVKQEIAEMRIPALNAYMKSLLSLPVWVLMDEDVRIFFYQSPYDSEQVPQALRRLRPRTRKVKSVSPQGNSVDRMAAPRAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKILKDFPEEDDPTNWLRCYYYEDTISTIKDIAVEEDLSSTPLLKDLLELTRREFQREDIALNYRDAEGDLVRLLSDEDVALMVRQARGLPSQKRLFPWKLHITQKDNYRVYNTMP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15080 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S11 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| T47 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| Y53 | Phosphorylation | Uniprot | |
| T154 | Phosphorylation | Q05655 (PRKCD) , P17252 (PRKCA) | Uniprot |
| S161 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| Y243 | Phosphorylation | Uniprot | |
| Y245 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | P17252 (PRKCA) | Uniprot |
Research Backgrounds
Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
Cytoplasm>Cytosol. Endosome membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Peripheral membrane protein.
Expression is restricted to hematopoietic cells.
Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. NCF4 interacts primarily with NCF2 to form a complex with NCF1.
The PB1 domain mediates the association with NCF2/p67-PHOX.
The PX domain mediates interaction with membranes enriched in phosphatidylnositol 3-phosphate.
Research Fields
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
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