Phospho-eIF4G1 (Ser1185) Antibody - #AF7350

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Product: Phospho-eIF4G1 (Ser1185) Antibody
Catalog: AF7350
Description: Rabbit polyclonal antibody to Phospho-eIF4G1 (Ser1185)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 220kDa; 175kD(Calculated).
Uniprot: Q04637
RRID: AB_2843790

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 100ul $350 In stock
 200ul $450 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
Phospho-eIF4G1 (Ser1185) Antibody detects endogenous levels of eIF4G1 only when phosphorylated at Ser1185.
RRID:
AB_2843790
Cite Format: Affinity Biosciences Cat# AF7350, RRID:AB_2843790.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686A1451; eIF 4 gamma 1; eIF 4G 1; eIF 4G1; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; EIF4 gamma; EIF4F; EIF4G; EIF4G1; EIF4GI; Eukaryotic translation initiation factor 4 gamma 1; IF4G1_HUMAN; p220;

Immunogens

Immunogen:
Uniprot:

Q04637(IF4G1_HUMAN) >>Visit HPA database.

Gene(ID):
EIF4G1(1981)
Sequence:
MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPTPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q04637 As Substrate

Site PTM Type Enzyme
S15 Phosphorylation
T43 Phosphorylation
R48 Methylation
Y52 Phosphorylation
R55 Methylation
S61 O-Glycosylation
R65 Methylation
R73 Methylation
Y103 Phosphorylation
R110 Methylation
T179 Phosphorylation
K190 Acetylation
T202 Phosphorylation
S204 Phosphorylation
T205 Phosphorylation
T207 Phosphorylation
T211 Phosphorylation
T223 Phosphorylation
S276 Phosphorylation
T301 Phosphorylation
S304 Phosphorylation
Y311 Phosphorylation
S314 Phosphorylation
T318 Phosphorylation
S343 Phosphorylation
T349 Phosphorylation
S353 Phosphorylation
S450 Phosphorylation
S496 Phosphorylation
K519 Sumoylation
K524 Ubiquitination
K580 Ubiquitination
K593 Ubiquitination
Y594 Phosphorylation
K597 Ubiquitination
S598 Phosphorylation
K602 Ubiquitination
K609 Ubiquitination
K646 Ubiquitination
T647 Phosphorylation P24941 (CDK2)
T667 Phosphorylation
S669 Phosphorylation
R675 Methylation
T676 Phosphorylation
R681 Methylation
R685 Methylation
R694 Methylation
R702 Methylation
S704 Phosphorylation
K732 Ubiquitination
K735 Ubiquitination
K756 Ubiquitination
K770 Ubiquitination
K781 Ubiquitination
K795 Ubiquitination
K832 Ubiquitination
K863 Ubiquitination
R877 Methylation
K881 Ubiquitination
S895 Phosphorylation Q13177 (PAK2)
K900 Ubiquitination
K907 Ubiquitination
K925 Ubiquitination
K944 Ubiquitination
K950 Acetylation
K950 Ubiquitination
R954 Methylation
K964 Acetylation
K964 Ubiquitination
K967 Ubiquitination
K1019 Ubiquitination
K1026 Methylation
K1026 Ubiquitination
S1028 Phosphorylation
R1031 Methylation
R1032 Methylation
R1042 Methylation
T1054 Phosphorylation
K1059 Methylation
K1059 Ubiquitination
T1066 Phosphorylation
K1071 Ubiquitination
T1073 Phosphorylation
K1074 Ubiquitination
S1077 Phosphorylation
S1080 Phosphorylation
S1092 Phosphorylation
K1095 Acetylation
K1095 Methylation
S1097 Phosphorylation
S1098 Phosphorylation
S1101 Phosphorylation
K1104 Methylation
S1106 Phosphorylation
S1108 Phosphorylation
S1110 Phosphorylation
T1117 Phosphorylation
T1119 Phosphorylation
R1138 Methylation
S1144 Phosphorylation
S1145 Phosphorylation
S1147 Phosphorylation P41279 (MAP3K8)
S1148 Phosphorylation
R1166 Methylation
T1179 Phosphorylation
S1185 Phosphorylation P17252 (PRKCA)
S1187 Phosphorylation
K1188 Acetylation
K1188 Ubiquitination
S1192 Phosphorylation
S1194 Phosphorylation
S1199 Phosphorylation
S1209 Phosphorylation
T1211 Phosphorylation
R1216 Methylation
S1231 Phosphorylation P06493 (CDK1)
K1234 Ubiquitination
S1238 Phosphorylation
K1244 Acetylation
K1244 Ubiquitination
Y1253 Phosphorylation
C1265 S-Nitrosylation
K1404 Ubiquitination
K1421 Ubiquitination
Y1424 Phosphorylation
T1425 Phosphorylation
S1430 Phosphorylation
R1436 Methylation
K1452 Ubiquitination
K1513 Ubiquitination
Y1526 Phosphorylation
T1534 Phosphorylation
Y1550 Phosphorylation
K1567 Ubiquitination
K1580 Ubiquitination
T1583 Phosphorylation
S1596 Phosphorylation

Research Backgrounds

Function:

Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.

PTMs:

Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1.

Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.

Subunit Structure:

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E (cap-binding) and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with CIRBP and MIF4GD. Interacts with RBM4. Interacts with HNRNPD/AUF1; the interaction requires RNA.

(Microbial infection) Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis.

(Microbial infection) Interacts with human adenovirus 5 protein 100K; this interaction promotes translational shunt in presence of polysomes containing viral tripartite leader mRNAs.

Family&Domains:

Belongs to the eukaryotic initiation factor 4G family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

References

1). Anti-inflammatory action of physalin A by blocking the activation of NF-κB signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY, 2021 (PubMed: 33091501) [IF=5.4]

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