Product: Phospho-EFNB1 (Tyr313/Tyr317) Antibody
Catalog: AF7340
Description: Rabbit polyclonal antibody to Phospho-EFNB1 (Tyr313/Tyr317)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 38kDa; 38kD(Calculated).
Uniprot: P98172
RRID: AB_2843780

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(89%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-EFNB1 (Tyr313/Tyr317) Antibody detects endogenous levels of EFNB1 only when phosphorylated at Tyr313/317.
RRID:
AB_2843780
Cite Format: Affinity Biosciences Cat# AF7340, RRID:AB_2843780.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CFND; CFNS; Craniofrontonasal syndrome (craniofrontonasal dysplasia); EFL 3; EFL-3; EFL3; EFNB1; EFNB1_HUMAN; Elk L; ELK ligand; ELK-L; Eph related receptor tyrosine kinase ligand 2; EPH-related receptor tyrosine kinase ligand 2; Ephrin-B1; EPLG2; LERK 2; LERK-2; LERK2; Ligand of eph related kinase 2; MGC8782;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P98172 EFNB1_HUMAN:

Widely expressed (PubMed:8070404, PubMed:7973638). Detected in both neuronal and non-neuronal tissues (PubMed:8070404, PubMed:7973638). Seems to have particularly strong expression in retina, sciatic nerve, heart and spinal cord (PubMed:7973638).

Sequence:
MARPGQRWLGKWLVAMVVWALCRLATPLAKNLEPVSWSSLNPKFLSGKGLVIYPKIGDKLDIICPRAEAGRPYEYYKLYLVRPEQAAACSTVLDPNVLVTCNRPEQEIRFTIKFQEFSPNYMGLEFKKHHDYYITSTSNGSLEGLENREGGVCRTRTMKIIMKVGQDPNAVTPEQLTTSRPSKEADNTVKMATQAPGSRGSLGDSDGKHETVNQEEKSGPGASGGSSGDPDGFFNSKVALFAAVGAGCVIFLLIIIFLTVLLLKLRKRHRKHTQQRAAALSLSTLASPKGGSGTAGTEPSDIIIPLRTTENNYCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
89
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P98172 As Substrate

Site PTM Type Enzyme
Y79 Phosphorylation
K163 Ubiquitination
T172 O-Glycosylation
T177 O-Glycosylation
T178 O-Glycosylation
S179 O-Glycosylation
T188 O-Glycosylation
S201 O-Glycosylation
T211 O-Glycosylation
S218 Phosphorylation
S223 Phosphorylation
S226 Phosphorylation
S227 Phosphorylation
S281 Phosphorylation
S283 Phosphorylation
T284 Phosphorylation
S287 Phosphorylation
K289 Ubiquitination
Y313 Phosphorylation
Y317 Phosphorylation
K319 Ubiquitination
S321 Phosphorylation
Y324 Phosphorylation
Y329 Phosphorylation
S338 Phosphorylation
Y343 Phosphorylation
Y344 Phosphorylation
K345 Ubiquitination

Research Backgrounds

Function:

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring cells. Shows high affinity for the receptor tyrosine kinase EPHB1/ELK. Can also bind EPHB2 and EPHB3. Binds to, and induces collapse of, commissural axons/growth cones in vitro (By similarity). May play a role in constraining the orientation of longitudinally projecting axons (By similarity).

PTMs:

Inducible phosphorylation of tyrosine residues in the cytoplasmic domain.

Proteolytically processed. The ectodomain is cleaved, probably by a metalloprotease, to produce a membrane-tethered C-terminal fragment. This fragment is then further processed by the gamma-secretase complex to yield a soluble intracellular domain peptide which can translocate to the nucleus. The intracellular domain peptide is highly labile suggesting that it is targeted for degradation by the proteasome.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Membrane raft.
Note: May recruit GRIP1 and GRIP2 to membrane raft domains.

Cell membrane>Single-pass type I membrane protein.

Nucleus.
Note: Colocalizes with ZHX2 in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Detected in both neuronal and non-neuronal tissues. Seems to have particularly strong expression in retina, sciatic nerve, heart and spinal cord.

Subunit Structure:

Interacts (via PDZ-binding motif) with GRIP1 and GRIP2 (via PDZ domain 6). Interacts with TLE1. The intracellular domain peptide interacts with ZHX2; the interaction enhances ZHX2 transcriptional repression activity (By similarity).

Family&Domains:

Belongs to the ephrin family.

Research Fields

· Organismal Systems > Development > Axon guidance.   (View pathway)

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