Phospho-Afadin (Tyr1230) Antibody - #AF7325

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Product: Phospho-Afadin (Tyr1230) Antibody
Catalog: AF7325
Description: Rabbit polyclonal antibody to Phospho-Afadin (Tyr1230)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 207kDa; 207kD(Calculated).
Uniprot: P55196
RRID: AB_2843765

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 100ul $350 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
Phospho-Afadin (Tyr1230) Antibody detects endogenous levels of Afadin only when phosphorylated at Tyr1230.
RRID:
AB_2843765
Cite Format: Affinity Biosciences Cat# AF7325, RRID:AB_2843765.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AF6; AFAD_HUMAN; Afadin; ALL1-fused gene from chromosome 6 protein; Mllt4; Protein Af-6; Protein AF6;

Immunogens

Immunogen:
Uniprot:

P55196(AFAD_HUMAN) >>Visit HPA database.

Gene(ID):
AFDN(4301)
Sequence:
MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Pig
90
Xenopus
89
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55196 As Substrate

Site PTM Type Enzyme
Y94 Phosphorylation
T160 Phosphorylation
S162 Phosphorylation
R178 Methylation
S181 Phosphorylation
Y203 Phosphorylation
S210 Phosphorylation
T214 Phosphorylation
S216 Phosphorylation
S240 Phosphorylation
S246 Phosphorylation
T249 Phosphorylation
Y253 Phosphorylation
S256 Phosphorylation
Y263 Phosphorylation
Y295 Phosphorylation
Y375 Phosphorylation
S391 Phosphorylation
S424 Phosphorylation
T426 Phosphorylation
S512 Phosphorylation
S551 Phosphorylation
S557 Phosphorylation
S561 Phosphorylation
S562 Phosphorylation
S564 Phosphorylation
S565 Phosphorylation
T566 Phosphorylation
Y584 Phosphorylation
S589 Phosphorylation
S655 Phosphorylation
S672 Phosphorylation
T909 Phosphorylation P11274 (BCR)
S973 Phosphorylation
T976 Phosphorylation
Y980 Phosphorylation
Y986 Phosphorylation
T995 Phosphorylation
T1011 Phosphorylation
K1014 Methylation
K1042 Ubiquitination
T1081 Phosphorylation
S1083 Phosphorylation
T1086 Phosphorylation
Y1096 Phosphorylation
T1101 Phosphorylation
S1107 Phosphorylation
R1117 Methylation
S1119 Phosphorylation
S1126 Phosphorylation
Y1132 Phosphorylation
S1135 Phosphorylation
T1136 Phosphorylation
S1140 Phosphorylation
S1143 Phosphorylation
Y1151 Phosphorylation
S1172 Phosphorylation
S1173 Phosphorylation
S1182 Phosphorylation
K1186 Methylation
S1187 Phosphorylation
Y1189 Phosphorylation
S1191 Phosphorylation
T1194 Phosphorylation
S1199 Phosphorylation
S1201 Phosphorylation
T1202 Phosphorylation
T1207 Phosphorylation
T1211 Phosphorylation
Y1219 Phosphorylation
Y1226 Phosphorylation
Y1230 Phosphorylation
T1232 Phosphorylation
S1236 Phosphorylation
S1238 Phosphorylation
Y1252 Phosphorylation
S1265 Phosphorylation
S1266 Phosphorylation
S1275 Phosphorylation
Y1285 Phosphorylation
S1300 Phosphorylation
S1302 Phosphorylation
S1328 Phosphorylation
T1330 Phosphorylation
S1333 Phosphorylation
T1334 Phosphorylation
T1352 Phosphorylation P24941 (CDK2)
Y1419 Phosphorylation
S1455 Phosphorylation
T1458 Phosphorylation
T1489 Phosphorylation
Y1497 Phosphorylation
T1499 Phosphorylation
S1501 Phosphorylation
S1506 Phosphorylation
S1512 Phosphorylation
Y1656 Phosphorylation
Y1657 Phosphorylation
S1658 Phosphorylation
Y1692 Phosphorylation
S1696 Phosphorylation
S1698 Phosphorylation
K1716 Methylation
S1721 Phosphorylation
S1772 Phosphorylation
S1774 Phosphorylation
S1779 Phosphorylation
S1782 Phosphorylation
S1783 Phosphorylation
S1799 Phosphorylation
S1805 Phosphorylation
T1815 Phosphorylation
T1823 Phosphorylation

Research Backgrounds

Function:

Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs) (By similarity). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm (By similarity). Essential for the organization of adherens junctions.

Subcellular Location:

Cell junction>Adherens junction.
Note: Not found at cell-matrix AJs.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Interacts with F-actin, nectin and NECTIN3. Essential for the association of nectin and E-cadherin. Isoform 1/s-afadin does not interact with F-actin. Interacts with ZO-1 and occludin, but probably in an indirect manner. Interacts with RIT1 and RIT2 (By similarity). Interacts with NRXN1 and BCR. Interacts with ADAM10; the interaction locks ADAM10 at adherens junctions following ADAM10 recruitment to adherens junctions by TSPAN33.

Family&Domains:

The PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich C-terminal domain interacts with F-actin.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

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